| Detailed Information for Phage Skipitt |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Julia Pitino |
| Year Found | 2014 |
| Location Found | Hartford, CT USA |
| Finding Institution | Trinity College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 41.74481 N, 72.690621 W Map |
| Discovery Notes | -Soil located in small garden plot in front of stairs leading to Life Sciences Center of Trinity College
-Dry soil
-Other plants in garden include
-ragweed
-mosses
-many weed varieties
-sample was taken from surface layer of soil to about one inch deep
|
| Naming Notes | Named after childhood toy called Skip-It, holds resemblance to toy in morphology. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 6, 2015 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 407 |
| Genome length (bp) | 51365 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGCCGGTAA |
| GC Content | 63.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | A |
| Subcluster | A4 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Trinity College |
| Annotation Status | In GenBank |
| Plaque Notes | After 48 hours:
-slightly cloudy
-defined edges
-1-3mm in diameter, average diameter: 2 mm |
| Morphotype | Siphoviridae |
| Number of Genes | 87 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH020249 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1.4 X 10^8 pfu/mL |
| Date of SEA Lysate Titering |
Oct 2, 2014 |
| Pitt Freezer Box# |
7 |
| Pitt Freezer Box Grid# |
C1 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
