| Detailed Information for Phage Snape |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Kara Jones |
| Year Found | 2014 |
| Location Found | New Haven, CT USA |
| Finding Institution | Southern Connecticut State University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Unknown |
| Isolation Temperature | 42°C |
| GPS Coordinates | 41.334278 N, 72.945667 W Map |
| Discovery Notes | This sample was found in ordinary dry soil found near the base of a tree.
|
| Naming Notes | This phage was named after J.K Rowling's most illusive and unpredictable character in the Harry Potter
Series--Severus Snape. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Mar 19, 2015 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 3623 |
| Genome length (bp) | 52138 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGTCGGTTA |
| End Determination Notes | Large buildups of reads were visible in Illumina sequencing data. |
| GC Content | 63.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | A |
| Subcluster | A11 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of the Sciences (now St. Joseph's Univ) |
| Annotation Status | In GenBank |
| Plaque Notes | 3.5-4 mm plaques & Lytic |
| Morphotype | Siphoviridae |
| Number of Genes | 99 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH536828 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Designator |
2014SCSUsnapeKJ |
| SEA Lysate Titer |
4.885 x 10^11 |
| Date of SEA Lysate Titering |
Nov 11, 2014 |
| Pitt Freezer Box# |
10 |
| Pitt Freezer Box Grid# |
C1 |
| Available Files |
| GenBank File for Phamerator | Download |