Mycobacterium phage Spike509
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Detailed Information for Phage Spike509
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByJacob Gries
Year Found2012
Location FoundSpokane, WA USA
Finding InstitutionGonzaga University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates47.667918 N, 117.404191 W Map
Discovery NotesCollected Sept 9, 2012. Moist soil with some bark dust. 5 cm depth, under bark dust. 26.7 deg C air temperature.
Naming NotesSpike is the name of the Gonzaga Bulldog. 509 is the area code.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedFeb 5, 2013
Sequencing FacilityVirginia Commonwealth University Nucleic Acids Research Facilities
Shotgun Sequencing Method454 Pyrosequencing
Approximate Shotgun Coverage660
Sanger Finishing Reactions12
Genome length (bp)50989
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGGTGGTAA
GC Content64.1%
Fasta file available?Yes: Download fasta file
Characterization
ClusterA
SubclusterA3
Cluster Life CycleTemperate
Other Cluster Members

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Chargerpower (None)

Abbyshoes (A1)

Abrogate (A1)

Acme (A1)

Adahisdi (A1)

Aeneas (A1)

AFIS (A1)

Agaliana (A1)

Ajay (A1)

Alsfro (A1)

Altman (A1)

Alvin (A1)

Anglerfish (A1)

Applejack (A1)

Arcanine (A1)

Arlo (A1)

Ashballer (A1)

Atkinbua (A1)

BaconJack (A1)

Barriga (A1)

Beatrix (A1)

BeesKnees (A1)

Bethlehem (A1)

Bexan (A1)

Big3 (A1)

Bigfoot (A1)

BigMau (A1)

BigPaolini (A1)

BillKnuckles (A1)

Bircsak (A1)

Blue (A1)

BluSpix (A1)

Bob3 (A1)

Bones (A1)

BPBiebs31 (A1)

Briton15 (A1)

Bruns (A1)

Burton (A1)

Buttons (A1)

Bxb1 (A1)

CactusRose (A1)

Carlyle (A1)

Chanagan (A1)

Ciao (A1)

ConceptII (A1)

Corvo (A1)

Crispicous1 (A1)

Cueylyss (A1)

DD5 (A1)

Dexes (A1)

Doom (A1)

Dreamboat (A1)

DreamCatcher (A1)

DrFeelGood (A1)

Dulcie (A1)

Dussy (A1)

Dynamix (A1)

Edtherson (A1)

Espresso (A1)

Euphoria (A1)

Eyeball (A1)

Fajezeel (A1)

Fascinus (A1)

Fenn (A1)

Forsytheast (A1)

Francis47 (A1)

Froghopper (A1)

Fushigi (A1)

GageAP (A1)

Gandalf20 (A1)

Gompeii16 (A1)

Graduation (A1)

GrecoEtereo (A1)

Greg (A1)

Gwendoluna (A1)

Gyzlar (A1)

Hami1 (A1)

HanShotFirst (A1)

HarryOW (A1)

Hermia (A1)

HermioneGrange (A1)

Homines (A1)

Hope4ever (A1)

Ichabod (A1)

IgnatiusPatJac (A1)

ILeeKay (A1)

Inyanga (A1)

JackSparrow (A1)

Jasper (A1)

JC27 (A1)

Jerm2 (A1)

Jorgensen (A1)

JuliaChild (A1)

Kanely (A1)

KBG (A1)

Kenmech (A1)

Killigrew (A1)

KSSJEB (A1)

Kugel (A1)

Kykar (A1)

KyMonks1A (A1)

Lamina13 (A1)

Lesedi (A1)

Licorice (A1)

LilBib (A1)

Lockley (A1)

Lopton (A1)

Magnar (A1)

Magnito (A1)

Makemake (A1)

Manatee (A1)

Marcell (A1)

Marchy (A1)

Marge (A1)

Maroc7 (A1)

Marsha (A1)

MaryBeth (A1)

McGuire (A1)

MetalQZJ (A1)

Michley (A1)

Mkhuseli (A1)

Molly (A1)

Monet (A1)

Moose (A1)

MPlant7149 (A1)

MrGordo (A1)

Mryolo (A1)

Mule (A1)

Museum (A1)

Naira (A1)

NEHalo (A1)

Nerujay (A1)

Nhonho (A1)

Niza (A1)

Norz (A1)

Ohno789 (A1)

Oogway (A1)

PacerPaul (A1)

Papez (A1)

Paphu (A1)

Paraselene (A1)

Pari (A1)

Parliament (A1)

PascalRango (A1)

PattyP (A1)

Payneful (A1)

Pelly (A1)

Pepe (A1)

Perseus (A1)

Peterson (A1)

Petp2012 (A1)

Petruchio (A1)

PherrisBueller (A1)

PhrostyMug (A1)

PinkPlastic (A1)

Pinto (A1)

Pippin (A1)

Pita2 (A1)

ProMouse (A1)

PSullivan (A1)

QTRlifeCrisis (A1)

Rajelicia (A1)

Rhynn (A1)

RidgeCB (A1)

Ringer (A1)

Rohr (A1)

Rubeus (A1)

Rufus (A1)

Ruotula (A1)

Rutherferd (A1)

Sagefire (A1)

Sandaddy (A1)

Sanya (A1)

SarFire (A1)

Scowl (A1)

Seabiscuit (A1)

Seanderson (A1)

ShortQueendom (A1)

Sibs6 (A1)

SkiPole (A1)

Slagathor (A1)

Smairt (A1)

Smeagol (A1)

Snazzy (A1)

Solon (A1)

Sorpresa (A1)

SpikeBT (A1)

Squee (A1)

StewieG (A1)

STLscum (A1)

StrongArm (A1)

Sumter (A1)

Sunshine924 (A1)

SwissCheese (A1)

Switzer (A1)

Swole (A1)

Target (A1)

Tasp14 (A1)

Teodoridan (A1)

TheloniousMonk (A1)

Thor (A1)

Topgun (A1)

Tote (A1)

Traft412 (A1)

Treddle (A1)

Tripl3t (A1)

Trouble (A1)

Turj99 (A1)

TwoPeat (A1)

U2 (A1)

Violet (A1)

Watermelon (A1)

Wheeler (A1)

Wilkins (A1)

Zeeculate (A1)

Zephyr (A1)

Zeuska (A1)

Chupacabra (A10)

DontArgue (A10)

Drake94 (A10)

DustyMartin (A10)

Eaglepride (A10)

Edison31 (A10)

Goose (A10)

KittenMittens (A10)

Kristoff (A10)

OKCentral2016 (A10)

PeaceMeal1 (A10)

Poompha (A10)

Rebeuca (A10)

RhynO (A10)

Rowdy (A10)

Severus (A10)

Shapes (A10)

Topanga (A10)

Trike (A10)

Twister (A10)

WalterMcMickey (A10)

Aneem (A11)

Bachome (A11)

Bowtie (A11)

Bud (A11)

Ebony (A11)

Et2Brutus (A11)

Fibonacci (A11)

Flaverint (A11)

Gilberta (A11)

Hutc2 (A11)

Insomnia (A11)

Jabith (A11)

Joselito (A11)

Lucivia (A11)

Mabel (A11)

MaCh (A11)

Mulciber (A11)

Munch (A11)

Orange (A11)

Petersenfast (A11)

Salz (A11)

Sham4 (A11)

Snape (A11)

Timothy (A11)

TinyTimmy (A11)

DarthPhader (A12)

Pharaoh (A12)

Refuge (A12)

Steamy (A12)

Zimmer (A12)

Phlei (A13)

Gail (A14)

Jeeves (A14)

Luchador (A14)

SororFago (A14)

Sunhee (A14)

Anaysia (A15)

Anon (A15)

Battleship (A15)

Boohoo (A15)

DekHockey33 (A15)

Epsocamisio (A15)

JSwag (A15)

KatherineG (A15)

LastResort (A15)

Looper (A15)

MinecraftSteve (A15)

Nebulosus (A15)

Nedarya (A15)

Oofda (A15)

ReMo (A15)

Remus (A15)

Rosalind (A15)

ShayRa (A15)

Soups (A15)

Strosahl (A15)

Switzerland (A15)

Waits (A15)

EagleEye (A16)

Lucyedi (A16)

PainterBoy (A16)

40AC (A17)

MyraDee (A18)

Kimona (A19)

20ES (A2)

AbbyPaige (A2)

Acolyte (A2)

Adzzy (A2)

Agape74 (A2)

AN3 (A2)

AN9 (A2)

ANI8 (A2)

AnnaL29 (A2)

Anselm (A2)

ArcherNM (A2)

BabyBack (A2)

Bactobuster (A2)

Baehexic (A2)

BengiVuitton (A2)

Benvolio (A2)

BiancaTri92 (A2)

BobSwaget (A2)

Bradman (A2)

Bugsy (A2)

Caraxes (A2)

Centaur (A2)

Changeling (A2)

Che12 (A2)

ChipMunk (A2)

Colin (A2)

CRB1 (A2)

Crucio (A2)

D29 (A2)

D32 (A2)

Dalmatian (A2)

DBQu4n (A2)

Deloris (A2)

Dignity (A2)

Drake55 (A2)

DudeLittle (A2)

Duplo (A2)

Echild (A2)

Equemioh13 (A2)

EvilGenius (A2)

EZMoney23 (A2)

Fameo (A2)

FiringLine (A2)

First (A2)

Flare16 (A2)

GaugeLDP (A2)

Georgie2 (A2)

HarryHoudini (A2)

Heffalump (A2)

Herbertwm (A2)

IronMan (A2)

Iwokeuplikedis (A2)

Jaan (A2)

Jerm (A2)

JoshKayV (A2)

Journey13 (A2)

Jsquared (A2)

Kalpine (A2)

Kerberos (A2)

KingCyrus (A2)

Koduck (A2)

L5 (A2)

LadyBird (A2)

Landor (A2)

Larenn (A2)

Leogania (A2)

Leviathan (A2)

LilTurb (A2)

Lokk (A2)

Loser (A2)

MajorMajor (A2)

Malec (A2)

MiculUcigas (A2)

Miko (A2)

MissWhite (A2)

Naji (A2)

NaSiaTalie (A2)

NicoleTera (A2)

NothingSpecial (A2)

Odin (A2)

Ph8s (A2)

Phaded (A2)

Piro94 (A2)

Pomar16 (A2)

Power (A2)

Pukovnik (A2)

QueenB2 (A2)

QueenBeesly (A2)

Quokka (A2)

Rachaly (A2)

RedRock (A2)

Retro23 (A2)

SemperFi (A2)

Serenity (A2)

Smeagan (A2)

SnapTap (A2)

StarStuff (A2)

Superchunk (A2)

SweetiePie (A2)

SwirlSquare (A2)

TarsusIV (A2)

TipsytheTRex (A2)

Tomathan (A2)

Travvers (A2)

Trixie (A2)

Trooper (A2)

Turbido (A2)

Updawg (A2)

VA6 (A2)

VC3 (A2)

WeiHuaDA (A2)

Whabigail7 (A2)

WideWale (A2)

XianYue (A2)

Anthony (A20)

Kinmap (A21)

ACFishhook (A3)

Aglet (A3)

AgronaGT15 (A3)

Anubis (A3)

AugsMagnumOpus (A3)

BabyJohn (A3)

BabyRay (A3)

Beauxregard13 (A3)

BlueBird (A3)

BreSam8 (A3)

Bugatti (A3)

BuzzBuzz (A3)

Bxz2 (A3)

Caviar (A3)

Chiqui (A3)

Colbster (A3)

Cullens (A3)

DaHudson (A3)

Daishi (A3)

Dieselweasel (A3)

DropBear (A3)

EpicPhail (A3)

Farber (A3)

Fernando (A3)

Fred313 (A3)

Gemma (A3)

GingkoMaracino (A3)

Giroux (A3)

Grif (A3)

Groupthink (A3)

Grub (A3)

Grum1 (A3)

GtownJaz (A3)

HashRod (A3)

HaveUMetTed (A3)

Heathen (A3)

HelDan (A3)

Heliosoles (A3)

Hercules11 (A3)

Hookmount (A3)

Idleandcovert (A3)

Isca (A3)

JenCasNa (A3)

JeppNRM (A3)

JF2 (A3)

JF4 (A3)

JHC117 (A3)

Jobu08 (A3)

Kachowdy (A3)

KADY (A3)

Kalb97 (A3)

Kalnoky (A3)

Lambert1 (A3)

LarryKay (A3)

LBerry (A3)

Lilith (A3)

Louie6 (A3)

LugYA (A3)

MA5 (A3)

MadMarie (A3)

Mainiac (A3)

Malinsilva (A3)

Manu (A3)

Marchesa (A3)

Margo (A3)

Marie (A3)

Marius (A3)

MarQuardt (A3)

Methuselah (A3)

Microwolf (A3)

Misomonster (A3)

MK4 (A3)

MoneyMay (A3)

MuchMore (A3)

Noella (A3)

Norbert (A3)

OlanP (A3)

Ollie (A3)

P28Green (A3)

Panamaxus (A3)

Pawn (A3)

Pembroke (A3)

Penny1 (A3)

PGHhamlin (A3)

Phantastic (A3)

PhishRPhriends (A3)

Phoebe (A3)

Phoxy (A3)

Phranny (A3)

Pistachio (A3)

Pocahontas (A3)

Popcicle (A3)

PotatoSplit (A3)

Puppy (A3)

PurpleHaze (A3)

QuinnKiro (A3)

Reba (A3)

ResDef (A3)

Rockstar (A3)

Rummer (A3)

Sabia (A3)

Sabinator (A3)

SaturnRing (A3)

Scout (A3)

Snickers (A3)

SoilDragon (A3)

Soshari (A3)

SoYo (A3)

Spike509 (A3)

Stagni (A3)

StepMih (A3)

Taurus (A3)

Texage (A3)

Tiffany (A3)

TNguyen7 (A3)

Todacoro (A3)

Veracruz (A3)

Vix (A3)

Watson (A3)

Wooldri (A3)

Zetzy (A3)

AbbysRanger (A4)

Abdiel (A4)

Achebe (A4)

Albee (A4)

Alberto7 (A4)

Annyong (A4)

Arturo (A4)

AvatarAhPeg (A4)

Avle17 (A4)

Baby16 (A4)

Backyardigan (A4)

Badger (A4)

BangNhom (A4)

Bartimeaus (A4)

BellusTerra (A4)

Blackmoor (A4)

Bombshell (A4)

Broseidon (A4)

Bruiser (A4)

BubbleTrouble (A4)

Bumblebee11 (A4)

Burger (A4)

Caelakin (A4)

Camperdownii (A4)

CentreCat (A4)

Cerulean (A4)

ChampagnePapi (A4)

Chaph (A4)

Cici (A4)

Cindaradix (A4)

Cintron (A4)

Citius (A4)

Clarenza (A4)

Cocoaberry (A4)

Commander (A4)

Connomayer (A4)

Datway (A4)

Deano (A4)

Dhanush (A4)

DirtyDunning (A4)

Druantia (A4)

Eagle (A4)

Eapen (A4)

Eris (A4)

Eros (A4)

Eurydice (A4)

Florean (A4)

Flux (A4)

Funston (A4)

Gadost (A4)

HamSlice (A4)

Happiness (A4)

Holli (A4)

Houdini22 (A4)

Iceman (A4)

ICleared (A4)

Iracema64 (A4)

Jaykayelowell (A4)

JetBlade (A4)

JoongJeon (A4)

Kampy (A4)

Katalie136 (A4)

KFPoly (A4)

Kingmustik0402 (A4)

Koreni (A4)

Kratark (A4)

Kremtemulon (A4)

Kyee (A4)

LappelDuVide (A4)

Lemur (A4)

LeoAvram (A4)

LHTSCC (A4)

LittleB (A4)

LittleGuy (A4)

LochMonster (A4)

Lorenzo (A4)

Lunsford (A4)

Maverick (A4)

Maxo (A4)

Mazhar510 (A4)

Medusa (A4)

MeeZee (A4)

Melvin (A4)

Millski (A4)

Miramae (A4)

Morpher26 (A4)

Morrow (A4)

Mundrea (A4)

Nebs (A4)

Nemo27 (A4)

Noelle (A4)

NorthStar (A4)

NotAPhaseMom (A4)

Nyxis (A4)

Obama12 (A4)

Ohfah (A4)

OKaNui (A4)

Palestino (A4)

Peaches (A4)

Perplexer (A4)

PeterPeter (A4)

PetiteSangsue (A4)

Phacado (A4)

Phelipe (A4)

Phighter1804 (A4)

Phontbonne (A4)

Pipcraft (A4)

Polymorphads (A4)

Pumbaa (A4)

Relief (A4)

Romney (A4)

Roosevelt (A4)

Rosa24 (A4)

Ruin (A4)

Sabertooth (A4)

Scamp (A4)

Shaka (A4)

SheldonCooper (A4)

Shygu2 (A4)

Skipitt (A4)

Sparxx (A4)

Spino (A4)

Stasia (A4)

Stink (A4)

Taquarus (A4)

Thanksgivukkah (A4)

TinaFeyge (A4)

Tinybot (A4)

TiroTheta9 (A4)

TroyPia (A4)

TygerBlood (A4)

Ulysses (A4)

Wander (A4)

Wilbur (A4)

Wile (A4)

Wizard007 (A4)

Xena (A4)

YoSam321 (A4)

AgentM (A5)

Airmid (A5)

Aragog (A5)

Archetta (A5)

Benedict (A5)

Bluefalcon (A5)

Bonamassa (A5)

Chadwick (A5)

Conspiracy (A5)

Coog (A5)

Cuco (A5)

Discoknowium (A5)

Dublin (A5)

ElTiger69 (A5)

ForGetIt (A5)

George (A5)

Ghoulboy (A5)

HuhtaEnerson15 (A5)

Jabiru (A5)

Jovo (A5)

Lev2 (A5)

LittleCherry (A5)

MarysWell (A5)

Micasa (A5)

Midas2 (A5)

Milcery (A5)

Naca (A5)

PetterN (A5)

Phlorence (A5)

PickleBack (A5)

Scorpia (A5)

Swirley (A5)

SydNat (A5)

Tarynearal (A5)

Theia (A5)

Tiger (A5)

UnionJack (A5)

Zolita (A5)

Artemis2UCLA (A6)

BABullseye (A6)

Blinn1 (A6)

Blue7 (A6)

Candra (A6)

Chartreuse (A6)

CloudWang3 (A6)

Cookiedough (A6)

DaVinci (A6)

Dorothea (A6)

EricB (A6)

Garak (A6)

Gladiator (A6)

GreedyLawyer (A6)

Gruunaga (A6)

Hammer (A6)

Helmet (A6)

Hexamo (A6)

Hoot (A6)

Indra (A6)

Isiphiwo (A6)

Jeffabunny (A6)

JewelBug (A6)

Jordennis (A6)

Kazan (A6)

Kipper29 (A6)

Koko (A6)

Lilbunny (A6)

McFly (A6)

Neeharika16 (A6)

Newrala (A6)

Pmask (A6)

Priamo (A6)

Rifter (A6)

Roksolana (A6)

SmellyB (A6)

SuperAwesome (A6)

SuperCallie99 (A6)

ToneTone (A6)

Tucker (A6)

VohminGhazi (A6)

Wiks (A6)

WunderPhul (A6)

Yokurt (A6)

Zaka (A6)

Zulu (A6)

DroogsArmy (A7)

FlyCatcher (A7)

HINdeR (A7)

Sheen (A7)

Timshel (A7)

Toro (A7)

Astro (A8)

Danforth (A8)

Dixon (A8)

Expelliarmus (A8)

Groundhog (A8)

NearlyHeadless (A8)

Phillis (A8)

Roary (A8)

Saintus (A8)

Smeadley (A8)

Stephig9 (A8)

Aliter (A9)

Alma (A9)

Arissanae (A9)

Beemo (A9)

BogosyJay (A9)

Catalina (A9)

Charm (A9)

Conquerage (A9)

Darrell (A9)

DreamTeam1 (A9)

EdogawaKiddo (A9)

Eidsmoe (A9)

Elephantoon (A9)

EmyBug (A9)

ExplosioNervosa (A9)

Fayely (A9)

Hanray (A9)

Holex (A9)

HortumSL17 (A9)

Jiawan (A9)

Keziacharles14 (A9)

Lilleskat (A9)

LoneWolf (A9)

Maminiaina (A9)

Myxus (A9)

Onglai (A9)

PackMan (A9)

Phaeder (A9)

Phonnegut (A9)

Pioneer (A9)

Priya (A9)

Qobbit (A9)

Rahalelujah (A9)

RyeScarlet (A9)

Sachima (A9)

Scherzo (A9)

SheaKeira (A9)

Spouty (A9)

Toaka (A9)

Tubs (A9)

Ugenie5 (A9)

Vanisoa (A9)

Yecey3 (A9)

Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
Plaque Notesturbid plaques
MorphotypeSiphoviridae
Number of Genes91
Number of tRNAs3
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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Spike509_1
(HNH endonuclease)

Spike509_2
(tail protein)

Spike509_3

Spike509_4
(collagen-like protein)

Spike509_5

Spike509_6

Spike509_7 — tRNA
(tRNA-Asn(gtt))

Spike509_8 — tRNA
(tRNA-Trp(cca))

Spike509_9 — tRNA
(tRNA-Leu(cag))

Spike509_10

Spike509_11
(lysin A)

Spike509_12
(lysin B)

Spike509_13
(terminase)

Spike509_14
(portal protein)

Spike509_15
(capsid maturation protease)

Spike509_16
(scaffolding protein)

Spike509_17
(major capsid protein)

Spike509_18
(head-to-tail connector protein)

Spike509_19
(head-to-tail adaptor)

Spike509_20

Spike509_21
(head-to-tail stopper)

Spike509_22

Spike509_23
(tail terminator)

Spike509_24
(major tail protein)

Spike509_26
(tail assembly chaperone)

Spike509_25
(tail assembly chaperone)

Spike509_27
(tape measure protein)

Spike509_28
(structural minor tail protein)

Spike509_29
(minor tail protein)

Spike509_30
(minor tail protein)

Spike509_31
(minor tail protein)

Spike509_32
(minor tail protein)

Spike509_33
(minor tail protein)

Spike509_34

Spike509_35

Spike509_36
(integrase)

Spike509_37

Spike509_38

Spike509_39

Spike509_40
(dCMP deaminase)

Spike509_41

Spike509_42

Spike509_43

Spike509_44

Spike509_45

Spike509_46

Spike509_47

Spike509_48
(DNA polymerase)

Spike509_49

Spike509_50
(HTH DNA binding domain protein)

Spike509_51

Spike509_52
(ThyX-like thymidylate synthase)

Spike509_53

Spike509_54
(ribonucleotide reductase)

Spike509_55

Spike509_56

Spike509_57
(putative sigma factor)

Spike509_58
(metallophosphoesterase)

Spike509_59

Spike509_60

Spike509_61

Spike509_62
(DNA primase)

Spike509_63
(DNA primase)

Spike509_64
(EndoVII)

Spike509_65

Spike509_66
(esterase/lipase)

Spike509_67

Spike509_68

Spike509_69
(DnaB-like dsDNA helicase)

Spike509_70

Spike509_71

Spike509_72

Spike509_73

Spike509_74

Spike509_75

Spike509_76

Spike509_77
(Cas4 exonuclease)

Spike509_78

Spike509_79
(immunity repressor)

Spike509_80

Spike509_81

Spike509_82

Spike509_83

Spike509_84

Spike509_85

Spike509_86

Spike509_87

Spike509_88

Spike509_89

Spike509_90

Spike509_91

Spike509_92

Spike509_93

Spike509_94

Submitted Annotation in DNA Master FormatDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKP017311
Refseq NumberNone yet
Published in PaperPope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status Archived
SEA Designator 2012GONZspike509JJG
SEA Lysate Titer 40x10^10
Date of SEA Lysate Titering Oct 19, 2012
Pitt Freezer Box# 36
Pitt Freezer Box Grid# 7
Available Files
Plaque PictureDownload
EM PictureDownload
Final DNAMaster FileDownload