Mycobacterium phage Zephyr
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| Detailed Information for Phage Zephyr | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Alexander Lu and Brandon Tran |
| Year Found | 2011 |
| Location Found | St. Louis, MO USA |
| Finding Institution | Washington University in St. Louis |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 38.645911 N, 90.314604 W Map |
| Discovery Notes | Wet, muddy soil, approximately 1 cm deep, at 23 degrees Celsius |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Oct 18, 2013 |
| Sequencing Facility | The McDonnell Genome Institute at Washington University |
| Shotgun Sequencing Method | 454 |
| Sequencer Used | 454 Genome Sequencer FLX |
| Approximate Shotgun Coverage | 250 |
| Sanger Finishing Reactions | 13 |
| Genome length (bp) | 52320 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGATGGTAA |
| GC Content | 63.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | A |
| Subcluster | A1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members | |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Average 6mm plaques Clear centers with cloudy rim |
| Morphotype | Siphoviridae |
| Number of Genes | 92 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List | |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KX712238 |
| Refseq Number | None yet |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info | |
| Archiving status | Archived |
| SEA Designator | 2011WUSLzephyrKH |
| SEA Lysate Titer | 1.14e8 |
| Date of SEA Lysate Titering | Sep 27, 2011 |
| Pitt Freezer Box# | 31 |
| Pitt Freezer Box Grid# | 73 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
