Mycobacterium phage Badger
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Detailed Information for Phage Badger
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByStevie Hoyng
Year Found2014
Location FoundBowling Green, KY USA
Finding InstitutionWestern Kentucky University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates36.988611 N, 86.448333 W Map
Discovery NotesThe soil came from my backyard from underneath a bird bath. The bird bath is heavily used by birds, as well as my neighbor’s cat. The soil had many different types of insects living it. The soil was very moist. The bird bath is located about 50 feet from a road that is heavily traveled by cars and people. Around the bird bath is plenty of grass and about two feet away is a row of day lilies. There is a lilac bush, Australian Pine Tree, and a Magnolia Tree in the yard.
Naming NotesI am from Pittsburgh and I am a huge hockey fan. My phage is named after "Badger Bob" Johnson who led the Pittsburgh Penguins to their first Stanley Cup Championship in 1991.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedDec 22, 2014
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage274
Genome length (bp)51274
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGCCGGTTA
GC Content63.7%
Fasta file available?Yes: Download fasta file
Characterization
ClusterA
SubclusterA4
Cluster Life CycleTemperate
Other Cluster Members

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Chargerpower (None)

Abbyshoes (A1)

Abrogate (A1)

Acme (A1)

Adahisdi (A1)

Aeneas (A1)

AFIS (A1)

Agaliana (A1)

Ajay (A1)

Alsfro (A1)

Altman (A1)

Alvin (A1)

Anglerfish (A1)

Applejack (A1)

Arcanine (A1)

Arlo (A1)

Ashballer (A1)

Atkinbua (A1)

BaconJack (A1)

Barriga (A1)

Beatrix (A1)

BeesKnees (A1)

Bethlehem (A1)

Bexan (A1)

Big3 (A1)

Bigfoot (A1)

BigMau (A1)

BigPaolini (A1)

BillKnuckles (A1)

Bircsak (A1)

Blue (A1)

BluSpix (A1)

Bob3 (A1)

Bones (A1)

BPBiebs31 (A1)

Briton15 (A1)

Bruns (A1)

Burton (A1)

Buttons (A1)

Bxb1 (A1)

CactusRose (A1)

Carlyle (A1)

Chanagan (A1)

Ciao (A1)

ConceptII (A1)

Corvo (A1)

Crispicous1 (A1)

Cueylyss (A1)

DD5 (A1)

Dexes (A1)

Doom (A1)

Dreamboat (A1)

DreamCatcher (A1)

DrFeelGood (A1)

Dulcie (A1)

Dussy (A1)

Dynamix (A1)

Edtherson (A1)

Espresso (A1)

Euphoria (A1)

Eyeball (A1)

Fajezeel (A1)

Fascinus (A1)

Fenn (A1)

Forsytheast (A1)

Francis47 (A1)

Froghopper (A1)

Fushigi (A1)

GageAP (A1)

Gandalf20 (A1)

Gompeii16 (A1)

Graduation (A1)

GrecoEtereo (A1)

Greg (A1)

Gwendoluna (A1)

Gyzlar (A1)

Hami1 (A1)

HanShotFirst (A1)

HarryOW (A1)

Hermia (A1)

HermioneGrange (A1)

Homines (A1)

Hope4ever (A1)

Ichabod (A1)

IgnatiusPatJac (A1)

ILeeKay (A1)

Inyanga (A1)

JackSparrow (A1)

Jasper (A1)

JC27 (A1)

Jerm2 (A1)

Jorgensen (A1)

JuliaChild (A1)

Kanely (A1)

KBG (A1)

Kenmech (A1)

Killigrew (A1)

KSSJEB (A1)

Kugel (A1)

Kykar (A1)

KyMonks1A (A1)

Lamina13 (A1)

Lesedi (A1)

Licorice (A1)

LilBib (A1)

Lockley (A1)

Lopton (A1)

Magnar (A1)

Magnito (A1)

Makemake (A1)

Manatee (A1)

Marcell (A1)

Marchy (A1)

Marge (A1)

Maroc7 (A1)

Marsha (A1)

MaryBeth (A1)

McGuire (A1)

MetalQZJ (A1)

Michley (A1)

Mkhuseli (A1)

Molly (A1)

Monet (A1)

Moose (A1)

MPlant7149 (A1)

MrGordo (A1)

Mryolo (A1)

Mule (A1)

Museum (A1)

Naira (A1)

NEHalo (A1)

Nerujay (A1)

Nhonho (A1)

Niza (A1)

Norz (A1)

Ohno789 (A1)

Oogway (A1)

PacerPaul (A1)

Papez (A1)

Paphu (A1)

Paraselene (A1)

Pari (A1)

Parliament (A1)

PascalRango (A1)

PattyP (A1)

Payneful (A1)

Pelly (A1)

Pepe (A1)

Perseus (A1)

Peterson (A1)

Petp2012 (A1)

Petruchio (A1)

PherrisBueller (A1)

PhrostyMug (A1)

PinkPlastic (A1)

Pinto (A1)

Pippin (A1)

Pita2 (A1)

ProMouse (A1)

PSullivan (A1)

QTRlifeCrisis (A1)

Rajelicia (A1)

Rhynn (A1)

RidgeCB (A1)

Ringer (A1)

Rohr (A1)

Rubeus (A1)

Rufus (A1)

Ruotula (A1)

Rutherferd (A1)

Sagefire (A1)

Sandaddy (A1)

Sanya (A1)

SarFire (A1)

Scowl (A1)

Seabiscuit (A1)

Seanderson (A1)

ShortQueendom (A1)

Sibs6 (A1)

SkiPole (A1)

Slagathor (A1)

Smairt (A1)

Smeagol (A1)

Snazzy (A1)

Solon (A1)

Sorpresa (A1)

SpikeBT (A1)

Squee (A1)

StewieG (A1)

STLscum (A1)

StrongArm (A1)

Sumter (A1)

Sunshine924 (A1)

SwissCheese (A1)

Switzer (A1)

Swole (A1)

Target (A1)

Tasp14 (A1)

Teodoridan (A1)

TheloniousMonk (A1)

Thor (A1)

Topgun (A1)

Tote (A1)

Traft412 (A1)

Treddle (A1)

Tripl3t (A1)

Trouble (A1)

Turj99 (A1)

TwoPeat (A1)

U2 (A1)

Violet (A1)

Watermelon (A1)

Wheeler (A1)

Wilkins (A1)

Zeeculate (A1)

Zephyr (A1)

Zeuska (A1)

Chupacabra (A10)

DontArgue (A10)

Drake94 (A10)

DustyMartin (A10)

Eaglepride (A10)

Edison31 (A10)

Goose (A10)

KittenMittens (A10)

Kristoff (A10)

OKCentral2016 (A10)

PeaceMeal1 (A10)

Poompha (A10)

Rebeuca (A10)

RhynO (A10)

Rowdy (A10)

Severus (A10)

Shapes (A10)

Topanga (A10)

Trike (A10)

Twister (A10)

WalterMcMickey (A10)

Aneem (A11)

Bachome (A11)

Bowtie (A11)

Bud (A11)

Ebony (A11)

Et2Brutus (A11)

Fibonacci (A11)

Flaverint (A11)

Gilberta (A11)

Hutc2 (A11)

Insomnia (A11)

Jabith (A11)

Joselito (A11)

Lucivia (A11)

Mabel (A11)

MaCh (A11)

Mulciber (A11)

Munch (A11)

Orange (A11)

Petersenfast (A11)

Salz (A11)

Sham4 (A11)

Snape (A11)

Timothy (A11)

TinyTimmy (A11)

DarthPhader (A12)

Pharaoh (A12)

Refuge (A12)

Steamy (A12)

Zimmer (A12)

Phlei (A13)

Gail (A14)

Jeeves (A14)

Luchador (A14)

SororFago (A14)

Sunhee (A14)

Anaysia (A15)

Anon (A15)

Battleship (A15)

Boohoo (A15)

DekHockey33 (A15)

Epsocamisio (A15)

JSwag (A15)

KatherineG (A15)

LastResort (A15)

Looper (A15)

MinecraftSteve (A15)

Nebulosus (A15)

Nedarya (A15)

Oofda (A15)

ReMo (A15)

Remus (A15)

Rosalind (A15)

ShayRa (A15)

Soups (A15)

Strosahl (A15)

Switzerland (A15)

Waits (A15)

EagleEye (A16)

Lucyedi (A16)

PainterBoy (A16)

40AC (A17)

MyraDee (A18)

Kimona (A19)

20ES (A2)

AbbyPaige (A2)

Acolyte (A2)

Adzzy (A2)

Agape74 (A2)

AN3 (A2)

AN9 (A2)

ANI8 (A2)

AnnaL29 (A2)

Anselm (A2)

ArcherNM (A2)

BabyBack (A2)

Bactobuster (A2)

Baehexic (A2)

BengiVuitton (A2)

Benvolio (A2)

BiancaTri92 (A2)

BobSwaget (A2)

Bradman (A2)

Bugsy (A2)

Caraxes (A2)

Centaur (A2)

Changeling (A2)

Che12 (A2)

ChipMunk (A2)

Colin (A2)

CRB1 (A2)

Crucio (A2)

D29 (A2)

D32 (A2)

Dalmatian (A2)

DBQu4n (A2)

Deloris (A2)

Dignity (A2)

Drake55 (A2)

DudeLittle (A2)

Duplo (A2)

Echild (A2)

Equemioh13 (A2)

EvilGenius (A2)

EZMoney23 (A2)

Fameo (A2)

FiringLine (A2)

First (A2)

Flare16 (A2)

GaugeLDP (A2)

Georgie2 (A2)

HarryHoudini (A2)

Heffalump (A2)

Herbertwm (A2)

IronMan (A2)

Iwokeuplikedis (A2)

Jaan (A2)

Jerm (A2)

JoshKayV (A2)

Journey13 (A2)

Jsquared (A2)

Kalpine (A2)

Kerberos (A2)

KingCyrus (A2)

Koduck (A2)

L5 (A2)

LadyBird (A2)

Landor (A2)

Larenn (A2)

Leogania (A2)

Leviathan (A2)

LilTurb (A2)

Lokk (A2)

Loser (A2)

MajorMajor (A2)

Malec (A2)

MiculUcigas (A2)

Miko (A2)

MissWhite (A2)

Naji (A2)

NaSiaTalie (A2)

NicoleTera (A2)

NothingSpecial (A2)

Odin (A2)

Ph8s (A2)

Phaded (A2)

Piro94 (A2)

Pomar16 (A2)

Power (A2)

Pukovnik (A2)

QueenB2 (A2)

QueenBeesly (A2)

Quokka (A2)

Rachaly (A2)

RedRock (A2)

Retro23 (A2)

SemperFi (A2)

Serenity (A2)

Smeagan (A2)

SnapTap (A2)

StarStuff (A2)

Superchunk (A2)

SweetiePie (A2)

SwirlSquare (A2)

TarsusIV (A2)

TipsytheTRex (A2)

Tomathan (A2)

Travvers (A2)

Trixie (A2)

Trooper (A2)

Turbido (A2)

Updawg (A2)

VA6 (A2)

VC3 (A2)

WeiHuaDA (A2)

Whabigail7 (A2)

WideWale (A2)

XianYue (A2)

Anthony (A20)

Kinmap (A21)

ACFishhook (A3)

Aglet (A3)

AgronaGT15 (A3)

Anubis (A3)

AugsMagnumOpus (A3)

BabyJohn (A3)

BabyRay (A3)

Beauxregard13 (A3)

BlueBird (A3)

BreSam8 (A3)

Bugatti (A3)

BuzzBuzz (A3)

Bxz2 (A3)

Caviar (A3)

Chiqui (A3)

Colbster (A3)

Cullens (A3)

DaHudson (A3)

Daishi (A3)

Dieselweasel (A3)

DropBear (A3)

EpicPhail (A3)

Farber (A3)

Fernando (A3)

Fred313 (A3)

Gemma (A3)

GingkoMaracino (A3)

Giroux (A3)

Grif (A3)

Groupthink (A3)

Grub (A3)

Grum1 (A3)

GtownJaz (A3)

HashRod (A3)

HaveUMetTed (A3)

Heathen (A3)

HelDan (A3)

Heliosoles (A3)

Hercules11 (A3)

Hookmount (A3)

Idleandcovert (A3)

Isca (A3)

JenCasNa (A3)

JeppNRM (A3)

JF2 (A3)

JF4 (A3)

JHC117 (A3)

Jobu08 (A3)

Kachowdy (A3)

KADY (A3)

Kalb97 (A3)

Kalnoky (A3)

Lambert1 (A3)

LarryKay (A3)

LBerry (A3)

Lilith (A3)

Louie6 (A3)

LugYA (A3)

MA5 (A3)

MadMarie (A3)

Mainiac (A3)

Malinsilva (A3)

Manu (A3)

Marchesa (A3)

Margo (A3)

Marie (A3)

Marius (A3)

MarQuardt (A3)

Methuselah (A3)

Microwolf (A3)

Misomonster (A3)

MK4 (A3)

MoneyMay (A3)

MuchMore (A3)

Noella (A3)

Norbert (A3)

OlanP (A3)

Ollie (A3)

P28Green (A3)

Panamaxus (A3)

Pawn (A3)

Pembroke (A3)

Penny1 (A3)

PGHhamlin (A3)

Phantastic (A3)

PhishRPhriends (A3)

Phoebe (A3)

Phoxy (A3)

Phranny (A3)

Pistachio (A3)

Pocahontas (A3)

Popcicle (A3)

PotatoSplit (A3)

Puppy (A3)

PurpleHaze (A3)

QuinnKiro (A3)

Reba (A3)

ResDef (A3)

Rockstar (A3)

Rummer (A3)

Sabia (A3)

Sabinator (A3)

SaturnRing (A3)

Scout (A3)

Snickers (A3)

SoilDragon (A3)

Soshari (A3)

SoYo (A3)

Spike509 (A3)

Stagni (A3)

StepMih (A3)

Taurus (A3)

Texage (A3)

Tiffany (A3)

TNguyen7 (A3)

Todacoro (A3)

Veracruz (A3)

Vix (A3)

Watson (A3)

Wooldri (A3)

Zetzy (A3)

AbbysRanger (A4)

Abdiel (A4)

Achebe (A4)

Albee (A4)

Alberto7 (A4)

Annyong (A4)

Arturo (A4)

AvatarAhPeg (A4)

Avle17 (A4)

Baby16 (A4)

Backyardigan (A4)

Badger (A4)

BangNhom (A4)

Bartimeaus (A4)

BellusTerra (A4)

Blackmoor (A4)

Bombshell (A4)

Broseidon (A4)

Bruiser (A4)

BubbleTrouble (A4)

Bumblebee11 (A4)

Burger (A4)

Caelakin (A4)

Camperdownii (A4)

CentreCat (A4)

Cerulean (A4)

ChampagnePapi (A4)

Chaph (A4)

Cici (A4)

Cindaradix (A4)

Cintron (A4)

Citius (A4)

Clarenza (A4)

Cocoaberry (A4)

Commander (A4)

Connomayer (A4)

Datway (A4)

Deano (A4)

Dhanush (A4)

DirtyDunning (A4)

Druantia (A4)

Eagle (A4)

Eapen (A4)

Eris (A4)

Eros (A4)

Eurydice (A4)

Florean (A4)

Flux (A4)

Funston (A4)

Gadost (A4)

HamSlice (A4)

Happiness (A4)

Holli (A4)

Houdini22 (A4)

Iceman (A4)

ICleared (A4)

Iracema64 (A4)

Jaykayelowell (A4)

JetBlade (A4)

JoongJeon (A4)

Kampy (A4)

Katalie136 (A4)

KFPoly (A4)

Kingmustik0402 (A4)

Koreni (A4)

Kratark (A4)

Kremtemulon (A4)

Kyee (A4)

LappelDuVide (A4)

Lemur (A4)

LeoAvram (A4)

LHTSCC (A4)

LittleB (A4)

LittleGuy (A4)

LochMonster (A4)

Lorenzo (A4)

Lunsford (A4)

Maverick (A4)

Maxo (A4)

Mazhar510 (A4)

Medusa (A4)

MeeZee (A4)

Melvin (A4)

Millski (A4)

Miramae (A4)

Morpher26 (A4)

Morrow (A4)

Mundrea (A4)

Nebs (A4)

Nemo27 (A4)

Noelle (A4)

NorthStar (A4)

NotAPhaseMom (A4)

Nyxis (A4)

Obama12 (A4)

Ohfah (A4)

OKaNui (A4)

Palestino (A4)

Peaches (A4)

Perplexer (A4)

PeterPeter (A4)

PetiteSangsue (A4)

Phacado (A4)

Phelipe (A4)

Phighter1804 (A4)

Phontbonne (A4)

Pipcraft (A4)

Polymorphads (A4)

Pumbaa (A4)

Relief (A4)

Romney (A4)

Roosevelt (A4)

Rosa24 (A4)

Ruin (A4)

Sabertooth (A4)

Scamp (A4)

Shaka (A4)

SheldonCooper (A4)

Shygu2 (A4)

Skipitt (A4)

Sparxx (A4)

Spino (A4)

Stasia (A4)

Stink (A4)

Taquarus (A4)

Thanksgivukkah (A4)

TinaFeyge (A4)

Tinybot (A4)

TiroTheta9 (A4)

TroyPia (A4)

TygerBlood (A4)

Ulysses (A4)

Wander (A4)

Wilbur (A4)

Wile (A4)

Wizard007 (A4)

Xena (A4)

YoSam321 (A4)

AgentM (A5)

Airmid (A5)

Aragog (A5)

Archetta (A5)

Benedict (A5)

Bluefalcon (A5)

Bonamassa (A5)

Chadwick (A5)

Conspiracy (A5)

Coog (A5)

Cuco (A5)

Discoknowium (A5)

Dublin (A5)

ElTiger69 (A5)

ForGetIt (A5)

George (A5)

Ghoulboy (A5)

HuhtaEnerson15 (A5)

Jabiru (A5)

Jovo (A5)

Lev2 (A5)

LittleCherry (A5)

MarysWell (A5)

Micasa (A5)

Midas2 (A5)

Milcery (A5)

Naca (A5)

PetterN (A5)

Phlorence (A5)

PickleBack (A5)

Scorpia (A5)

Swirley (A5)

SydNat (A5)

Tarynearal (A5)

Theia (A5)

Tiger (A5)

UnionJack (A5)

Zolita (A5)

Artemis2UCLA (A6)

BABullseye (A6)

Blinn1 (A6)

Blue7 (A6)

Candra (A6)

Chartreuse (A6)

CloudWang3 (A6)

Cookiedough (A6)

DaVinci (A6)

Dorothea (A6)

EricB (A6)

Garak (A6)

Gladiator (A6)

GreedyLawyer (A6)

Gruunaga (A6)

Hammer (A6)

Helmet (A6)

Hexamo (A6)

Hoot (A6)

Indra (A6)

Isiphiwo (A6)

Jeffabunny (A6)

JewelBug (A6)

Jordennis (A6)

Kazan (A6)

Kipper29 (A6)

Koko (A6)

Lilbunny (A6)

McFly (A6)

Neeharika16 (A6)

Newrala (A6)

Pmask (A6)

Priamo (A6)

Rifter (A6)

Roksolana (A6)

SmellyB (A6)

SuperAwesome (A6)

SuperCallie99 (A6)

ToneTone (A6)

Tucker (A6)

VohminGhazi (A6)

Wiks (A6)

WunderPhul (A6)

Yokurt (A6)

Zaka (A6)

Zulu (A6)

DroogsArmy (A7)

FlyCatcher (A7)

HINdeR (A7)

Sheen (A7)

Timshel (A7)

Toro (A7)

Astro (A8)

Danforth (A8)

Dixon (A8)

Expelliarmus (A8)

Groundhog (A8)

NearlyHeadless (A8)

Phillis (A8)

Roary (A8)

Saintus (A8)

Smeadley (A8)

Stephig9 (A8)

Aliter (A9)

Alma (A9)

Arissanae (A9)

Beemo (A9)

BogosyJay (A9)

Catalina (A9)

Charm (A9)

Conquerage (A9)

Darrell (A9)

DreamTeam1 (A9)

EdogawaKiddo (A9)

Eidsmoe (A9)

Elephantoon (A9)

EmyBug (A9)

ExplosioNervosa (A9)

Fayely (A9)

Hanray (A9)

Holex (A9)

HortumSL17 (A9)

Jiawan (A9)

Keziacharles14 (A9)

Lilleskat (A9)

LoneWolf (A9)

Maminiaina (A9)

Myxus (A9)

Onglai (A9)

PackMan (A9)

Phaeder (A9)

Phonnegut (A9)

Pioneer (A9)

Priya (A9)

Qobbit (A9)

Rahalelujah (A9)

RyeScarlet (A9)

Sachima (A9)

Scherzo (A9)

SheaKeira (A9)

Spouty (A9)

Toaka (A9)

Tubs (A9)

Ugenie5 (A9)

Vanisoa (A9)

Yecey3 (A9)

Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
Plaque NotesThe plaques have been bull eyes, they have been dark in the middle and clear on the edge. They have been about 1-3 mm in diameter. They are temperate.
MorphotypeSiphoviridae
Number of Genes85
Number of tRNAs0
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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Badger_1
(HNH endonuclease)

Badger_2

Badger_3

Badger_4
(minor tail protein)

Badger_5

Badger_6

Badger_7

Badger_8
(lysin A)

Badger_9
(holin)

Badger_10
(lysin B)

Badger_11
(terminase large subunit)

Badger_12
(portal protein)

Badger_13
(capsid maturation protease)

Badger_14
(scaffolding protein)

Badger_15
(major capsid protein)

Badger_16

Badger_17
(head-to-tail adaptor)

Badger_18

Badger_19
(head-to-tail stopper)

Badger_20

Badger_21
(tail terminator)

Badger_22
(major tail protein)

Badger_23
(tail assembly chaperone)

Badger_24
(tail assembly chaperone)

Badger_25
(tape measure protein)

Badger_26
(minor tail protein)

Badger_27
(minor tail protein)

Badger_28
(minor tail protein)

Badger_29
(minor tail protein)

Badger_30
(minor tail protein)

Badger_31
(minor tail protein)

Badger_32

Badger_33
(serine integrase)

Badger_34

Badger_35
(deoxycytidylate deaminase)

Badger_36

Badger_37
(helix-turn-helix DNA binding domain protein)

Badger_38

Badger_39

Badger_40

Badger_41

Badger_42

Badger_43
(DNA polymerase I)

Badger_44
(helix-turn-helix DNA-binding protein)

Badger_45

Badger_46
(ThyX-like thymidylate synthase)

Badger_47

Badger_48
(ribonucleotide reductase)

Badger_49

Badger_50
(metallophosphoesterase)

Badger_51

Badger_52

Badger_53
(DNA primase)

Badger_54
(DNA primase)

Badger_55
(endonuclease VII)

Badger_56

Badger_57
(hydrolase)

Badger_58

Badger_59

Badger_60
(phosphoribosyltransferase)

Badger_61

Badger_62
(DnaB-like dsDNA helicase)

Badger_63

Badger_64

Badger_65

Badger_66
(Cas4 exonuclease)

Badger_67

Badger_68
(immunity repressor)

Badger_69

Badger_70

Badger_71

Badger_72

Badger_73

Badger_74
(DNA methylase)

Badger_75
(DNA methylase)

Badger_76
(SprT-like protease)

Badger_77

Badger_78

Badger_79

Badger_80

Badger_81

Badger_82

Badger_83

Badger_84

Badger_85

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank Accession KX550442
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Designator 2014WEKUbadgerSH
SEA Lysate Titer 3.3 x 10^8
Date of SEA Lysate Titering Oct 16, 2014
Pitt Freezer Box# 10
Pitt Freezer Box Grid# C8
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
Final DNAMaster FileDownload
GenBank File for PhameratorDownload