Mycobacterium phage Benvolio
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| Detailed Information for Phage Benvolio | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Ryan Adams |
| Year Found | 2018 |
| Location Found | Blacksburg, VA USA |
| Finding Institution | Virginia Tech |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 37.242 N, 80.428 W Map |
| Discovery Notes | The phage was found near a small trail behind an apartment complex with somewhat heavy foot traffic. |
| Naming Notes | I named this phage after my favorite Shakespearean character: Benvolio. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Nov 30, 2018 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1729 |
| Genome length (bp) | 53136 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGTCGGTTA |
| GC Content | 63.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | A |
| Subcluster | A2 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members | |
| Annotating Institution | Virginia Tech |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques produced by this phage tend to be polymorphous with small and medium sizes. The plaques are typically temperate. |
| Morphotype | Siphoviridae |
| Number of Genes | 95 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List | |
| Submitted Minimal DNA Master File | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK919472 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 80 |
| Pitt Freezer Box Grid# | A7 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
