Detailed Information for Phage Blinn1 |
Discovery Information |
Isolation Host | Mycobacterium smegmatis mc²155 |
Found By | Antonio Banda-Trejo |
Year Found | 2018 |
Location Found | Sinton, TX USA |
Finding Institution | Del Mar College |
Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
From enriched soil sample? | Yes |
Isolation Temperature | Not entered |
GPS Coordinates | 28.059722 N, 97.53 W Map |
Discovery Notes | The soil sample was found on a 30 acre plot of land by Sinton TX. The sample was found at the base of a tree that had been cut down and had begun to sprout back. The soil was fairly dry upon discovery but was dark in color. There also seemed by small holes in the dirt where some bugs may have entered along with some cow feces near by. Most of the cows on the land are an Angus or Charolais mix. The weather conditions at the time where 89 degrees fahrenheit and 55 percent humidity. |
Naming Notes | Temporary name |
Sequencing Information |
Sequencing Complete? | Yes |
Date Sequencing Completed | Jan 11, 2019 |
Sequencing Facility | Pittsburgh Bacteriophage Institute |
Shotgun Sequencing Method | Illumina |
Approximate Shotgun Coverage | 1860 |
Genome length (bp) | 51800 |
Character of genome ends | 3' Sticky Overhang |
Overhang Length | 10 bases |
Overhang Sequence | CGGTCGGTTA |
GC Content | 61.2% |
Fasta file available? | Yes: Download fasta file |
Characterization |
Cluster | A |
Subcluster | A6 |
Cluster Life Cycle | Temperate |
Other Cluster Members |
|
Annotating Institution | University of Pittsburgh |
Annotation Status | In GenBank |
Plaque Notes | The phage plaque at its largest can grow about 6 to 7 millimeters in diameter. The plaque is clear in the center with a small cloudy halo around the edges. After the first round of phage assay the phage appears to be lytic. |
Morphotype | Siphoviridae |
Number of Genes | 97 |
Number of tRNAs | 3 |
Number of tmRNAs | 0 |
Has been Phamerated? | Yes |
Gene List |
|
Submitted Minimal DNA Master File | Download |
Publication Info |
Uploaded to GenBank? | Yes |
GenBank Accession | MN586039 |
Refseq Number | None yet |
Archiving Info |
Archiving status |
Archived |
Pitt Freezer Box# |
79 |
Pitt Freezer Box Grid# |
F9 |
Available Files |
Plaque Picture | Download |
EM Picture | Download |
Final DNAMaster File | Download |
GenBank File for Phamerator | Download |