Mycobacterium phage Centaur
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Skelbel (AZ1) Keough (EK2) MamaT (GI) Lilo27 (EB) Liberone (GI)Mycobacterium phage Centaur
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| Detailed Information for Phage Centaur | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Former names | A'2B3P |
| Found By | Amanda Staples |
| Year Found | 2014 |
| Location Found | Upton, KY USA |
| Finding Institution | Western Kentucky University |
| Program | Kentucky Biomedical Infrastructure Network Small Genomes Discovery Program |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 37.482447 N, 85.905523 W Map |
| Discovery Notes | Soil was collected next to swing set in front yard. Rural area surrounded by cow farms. The soil was on top of a concrete encased 4x4 post where it was continually agitated by digging for worms. The soil was light brown and dry (compared to the red clay that is normally found). |
| Naming Notes | The Centaur is the symbol of the Sagittarius, the Zodiac sign my youngest child. Just like my son, this plaque was very strong and determined. With each purification, this plaque continued to be visible. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Mar 14, 2017 |
| Sequencing Facility | NC State Genomic Sciences Laboratory |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 283 |
| Genome length (bp) | 53001 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGTCGGTTA |
| GC Content | 63.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | A |
| Subcluster | A2 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members | |
| Annotating Institution | Western Kentucky University |
| Annotation Status | In GenBank |
| Plaque Notes | Large turbid bulls-eyes that were more visible than other turbid plaques, yet still not completely clear. |
| Morphotype | Siphoviridae |
| Number of Genes | 97 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List | |
| Submitted Minimal DNA Master File | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK061408 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 7.1x10^8 |
| Date of SEA Lysate Titering | Dec 3, 2014 |
| Pitt Freezer Box# | 52 |
| Pitt Freezer Box Grid# | C9 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |