| Detailed Information for Phage DarthPhader |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Ciera Min and Chad Hicks |
| Year Found | 2014 |
| Location Found | Spokane, WA USA |
| Finding Institution | Gonzaga University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 25°C |
| GPS Coordinates | 47.666276 N, 117.402764 W Map |
| Discovery Notes | Date: 9/4/14
Time:10:14 AM
Weather: 55 degrees F; 12.7 degrees C
Humidity: 74%; Wind: N@3mph; Pressure: 30.19 in; Dew Point: 47 degrees; UV: 3-moderate
Location: single pine tree on the right side of Herak building with "Clark Kolloch Rice" monument - right around the stomp of the tree
Depth: 1 inch/2 cm deep from surface
Moisture: Damp
Site Features: Shaded; beside fern plant; slightly rocky
|
| Naming Notes | Since this phage kills Mycobacterium Smegmatis quite effectively, we altered the name "DarthVader." |
| Growth Notes | Isolated at room temperature |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 19, 2014 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 230 |
| Genome length (bp) | 53432 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGGCCGTAA |
| GC Content | 63.2% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | A |
| Subcluster | A12 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | 3-4 mm in diameter
Clear and defined edges
Titer: 1.26 X 10^10 pfu/mL
|
| Morphotype | Siphoviridae |
| Number of Genes | 91 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KX657793 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
16 |
| Pitt Freezer Box Grid# |
C11 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
