Mycobacterium phage Duplo
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Skelbel (AZ1) Keough (EK2) MamaT (GI) Lilo27 (EB) Liberone (GI)Mycobacterium phage Duplo
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| Detailed Information for Phage Duplo | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Skylar Weiss and Jimena Segovia |
| Year Found | 2020 |
| Location Found | Big Sandy, TX United States of America |
| Finding Institution | LeTourneau University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Unknown |
| Isolation Temperature | 22°C |
| GPS Coordinates | 32.588034 N, 95.063959 W Map |
| Discovery Notes | Collection of the sample occurred at 7:02 AM on Tuesday, August 25, 2020. The site was in full shade at this time and the surrounding ground was covered in a significant layer of dew. The location had not received rain in the prior week, but due to the nature of organic composting, the interior of the collection site had significant moisture content. |
| Naming Notes | As we progressed through the purification and amplification process, this phage began to demonstrate two distinct morphologies. After confirming that this wasn't a purity issue, it was determined that our phage had mutated and was showing both lytic and lysogenic properties. Because of this, we derived the name "duplo" meaning "double" in Latin. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 22, 2020 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1516 |
| Genome length (bp) | 52781 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGTCGGTTA |
| GC Content | 63.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | A |
| Subcluster | A2 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members | |
| Annotating Institution | LeTourneau University |
| Annotation Status | In GenBank |
| Morphotype | Siphoviridae |
| Number of Genes | 94 |
| Number of tRNAs | 5 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List | |
| Submitted Minimal DNA Master File | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | OP297553 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 118 |
| Pitt Freezer Box Grid# | C10 |
| Available Files | |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |