| Detailed Information for Phage Equemioh13 |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Leah Stetzel |
| Year Found | 2013 |
| Location Found | Oxford, OH USA |
| Finding Institution | Miami University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 39.508611 N, 84.724167 W Map |
| Discovery Notes | The sample was selected from the equestrian center at Miami University in the area that the horses practice. The sample was somewhat rocky in texture and also had some moisture because of previous rain. The sample was exposed to direct sunlight- there were no surrounding buildings or trees. The sample had a slight odor as well. |
| Naming Notes | Because I gathered the sample from the equestrian center at Miami of Ohio in 2013, I combined this to form Equemioh13. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 19, 2013 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 3717 |
| Genome length (bp) | 53042 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGTCGGTTA |
| GC Content | 63.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | A |
| Subcluster | A2 |
| Cluster Life Cycle | Temperate |
| Lysogeny Notes | lysogenic- more lytic towards the middle of the plaque and becomes increasingly lysogenic as you go out |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Lysogenic |
| Morphotype | Siphoviridae |
| Number of Genes | 96 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KJ959632 |
| Refseq Number | None yet |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1.18e10 |
| Date of SEA Lysate Titering |
Oct 16, 2013 |
| Pitt Freezer Box# |
26 |
| Pitt Freezer Box Grid# |
D12 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |