| Detailed Information for Phage GMonster |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Jenna Greene |
| Year Found | 2024 |
| Location Found | Providence, RI United States |
| Finding Institution | Providence College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 41.84393 N, 71.43444 W Map |
| Discovery Notes | The sample was collected outside of our dining hall in Providence, RI around 2:00 pm. The weather was 80 degree Fahrenheit (26.6 degrees Celsius) on a mostly cloudy but humid day. The soil was collected in a garden of clumpy rich soil under a layer of mulch. |
| Naming Notes | I wanted to show my appreciation to my family by using an iteration of our shared nickname, "The Greene Monster." We came up with it because we’re all die-hard Red Sox fans and bond over our love for baseball. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Nov 22, 2024 |
| Sequencing Facility | Rhode Island INBRE Molecular Informatics Core |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Paired-end reads |
| Read Length | 250 bp |
| Approximate Shotgun Coverage | 5136 |
| Genome length (bp) | 50575 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGATGGTAA |
| GC Content | 64.0% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | A |
| Subcluster | A1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Providence College |
| Annotation Status | QC Complete |
| Plaque Notes | Small plaques around 2 mm in diameter and were clear. |
| Morphotype | Siphoviridae |
| Number of Genes | 87 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
195 |
| Pitt Freezer Box Grid# |
F11 |
| Available Files |
| GenBank File for Phamerator | Download |