Mycobacterium phage Graduation
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Detailed Information for Phage Graduation
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByS Patel, L Harris, C Jun, G Rauscher, K Yu
Year Found2011
Location FoundLos Angeles, CA USA
Finding InstitutionUniversity of California, Los Angeles
ProgramUnavailable
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates34.0308 N, 118.1434 W Map
Discovery NotesSample collection was performed by Shabnum Patel, and then the phage isolation was performed by Liam Harris, Claire Jun, Glenn Rauscher, and Kenny Yu (spring 2011). DNA preparation performed by Krisanavane Reddi. Assembly performed by William Villella (UCLA) and confirmed by Charles Bowman (University of Pittsburgh). Preliminary genome annotation performed by Gilbert Laim, Daiki Okazaki, Sahar Tashakor, and Rui (Moses) Zhang (winter 2013). Curation of annotation results performed by Khristina Ipapo (UCLA, spring 2013) and Debbie Jacobs-Sera (University of Pittsburgh, summer 2013).

Soil sample collected from planter box outside of Covel Commons at UCLA on April 6, 2011.

Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedMay 13, 2013
Sequencing FacilityThe UCLA Genotyping and Sequencing Core
Shotgun Sequencing Method454
Approximate Shotgun Coverage336
Genome length (bp)52823
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGATGGTAA
GC Content63.5%
Fasta file available?Yes: Download fasta file
Characterization
ClusterA
SubclusterA1
Cluster Life CycleTemperate
Other Cluster Members

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Chargerpower (None)

Abbyshoes (A1)

Abrogate (A1)

Acme (A1)

Adahisdi (A1)

Aeneas (A1)

AFIS (A1)

Agaliana (A1)

Ajay (A1)

Alsfro (A1)

Altman (A1)

Alvin (A1)

Anglerfish (A1)

Applejack (A1)

Arcanine (A1)

Arlo (A1)

Ashballer (A1)

Atkinbua (A1)

BaconJack (A1)

Barriga (A1)

Beatrix (A1)

BeesKnees (A1)

Bethlehem (A1)

Bexan (A1)

Big3 (A1)

Bigfoot (A1)

BigMau (A1)

BigPaolini (A1)

BillKnuckles (A1)

Bircsak (A1)

Blue (A1)

BluSpix (A1)

Bob3 (A1)

Bones (A1)

BPBiebs31 (A1)

Briton15 (A1)

Bruns (A1)

Burton (A1)

Buttons (A1)

Bxb1 (A1)

CactusRose (A1)

Carlyle (A1)

Chanagan (A1)

Ciao (A1)

ConceptII (A1)

Corvo (A1)

Crispicous1 (A1)

Cueylyss (A1)

DD5 (A1)

Dexes (A1)

Doom (A1)

Dreamboat (A1)

DreamCatcher (A1)

DrFeelGood (A1)

Dulcie (A1)

Dussy (A1)

Dynamix (A1)

Edtherson (A1)

Espresso (A1)

Euphoria (A1)

Eyeball (A1)

Fajezeel (A1)

Fascinus (A1)

Fenn (A1)

Forsytheast (A1)

Francis47 (A1)

Froghopper (A1)

Fushigi (A1)

GageAP (A1)

Gandalf20 (A1)

GMonster (A1)

Gompeii16 (A1)

Graduation (A1)

GrecoEtereo (A1)

Greg (A1)

Gwendoluna (A1)

Gyzlar (A1)

Hami1 (A1)

HanShotFirst (A1)

HarryOW (A1)

Hermia (A1)

HermioneGrange (A1)

Homines (A1)

Hope4ever (A1)

Ichabod (A1)

IgnatiusPatJac (A1)

ILeeKay (A1)

Inyanga (A1)

JackSparrow (A1)

Jasper (A1)

JC27 (A1)

Jerm2 (A1)

Jorgensen (A1)

JuliaChild (A1)

Kanely (A1)

KBG (A1)

Kenmech (A1)

Killigrew (A1)

KSSJEB (A1)

Kugel (A1)

Kykar (A1)

KyMonks1A (A1)

Lamina13 (A1)

Lesedi (A1)

Licorice (A1)

LilBib (A1)

Lockley (A1)

Lopton (A1)

Magnar (A1)

Magnito (A1)

Makemake (A1)

Manatee (A1)

Marcell (A1)

Marchy (A1)

Marge (A1)

Maroc7 (A1)

Marsha (A1)

MaryBeth (A1)

McGuire (A1)

MetalQZJ (A1)

Michley (A1)

Mkhuseli (A1)

Molly (A1)

Monet (A1)

Moose (A1)

MPlant7149 (A1)

MrGordo (A1)

Mryolo (A1)

Mule (A1)

Museum (A1)

Naira (A1)

NEHalo (A1)

Nerujay (A1)

Nhonho (A1)

Niza (A1)

Norz (A1)

Ohno789 (A1)

Oogway (A1)

PacerPaul (A1)

Papez (A1)

Paphu (A1)

Paraselene (A1)

Pari (A1)

Parliament (A1)

PascalRango (A1)

PattyP (A1)

Payneful (A1)

Pelly (A1)

Pepe (A1)

Perseus (A1)

Peterson (A1)

Petp2012 (A1)

Petruchio (A1)

PherrisBueller (A1)

PhrostyMug (A1)

PinkPlastic (A1)

Pinto (A1)

Pippin (A1)

Pita2 (A1)

ProMouse (A1)

PSullivan (A1)

QTRlifeCrisis (A1)

Rajelicia (A1)

Rhynn (A1)

RidgeCB (A1)

Ringer (A1)

Rohr (A1)

Rubeus (A1)

Rufus (A1)

Ruotula (A1)

Rutherferd (A1)

Sagefire (A1)

Sandaddy (A1)

Sanya (A1)

SarFire (A1)

Scowl (A1)

Seabiscuit (A1)

Seanderson (A1)

ShortQueendom (A1)

Sibs6 (A1)

SkiPole (A1)

Slagathor (A1)

Smairt (A1)

Smeagol (A1)

Snazzy (A1)

Solon (A1)

Sorpresa (A1)

SpikeBT (A1)

Squee (A1)

StewieG (A1)

STLscum (A1)

StrongArm (A1)

Sumter (A1)

Sunshine924 (A1)

SwissCheese (A1)

Switzer (A1)

Swole (A1)

Target (A1)

Tasp14 (A1)

Teodoridan (A1)

TheloniousMonk (A1)

Thor (A1)

Topgun (A1)

Tote (A1)

Traft412 (A1)

Treddle (A1)

Tripl3t (A1)

Trouble (A1)

Turj99 (A1)

TwoPeat (A1)

U2 (A1)

Violet (A1)

Watermelon (A1)

Wheeler (A1)

Wilkins (A1)

Zeeculate (A1)

Zephyr (A1)

Zeuska (A1)

Chupacabra (A10)

DontArgue (A10)

Drake94 (A10)

DustyMartin (A10)

Eaglepride (A10)

Edison31 (A10)

Goose (A10)

KittenMittens (A10)

Kristoff (A10)

OKCentral2016 (A10)

PeaceMeal1 (A10)

Poompha (A10)

Rebeuca (A10)

RhynO (A10)

Rowdy (A10)

Severus (A10)

Shapes (A10)

Topanga (A10)

Trike (A10)

Twister (A10)

WalterMcMickey (A10)

Aneem (A11)

Bachome (A11)

Bowtie (A11)

Bud (A11)

Ebony (A11)

Et2Brutus (A11)

Fibonacci (A11)

Flaverint (A11)

Gilberta (A11)

Hutc2 (A11)

Insomnia (A11)

Jabith (A11)

Joselito (A11)

Lucivia (A11)

Mabel (A11)

MaCh (A11)

Mulciber (A11)

Munch (A11)

Orange (A11)

Petersenfast (A11)

Salz (A11)

Sham4 (A11)

Snape (A11)

Timothy (A11)

TinyTimmy (A11)

DarthPhader (A12)

Pharaoh (A12)

Refuge (A12)

Steamy (A12)

Zimmer (A12)

Phlei (A13)

Gail (A14)

Jeeves (A14)

Luchador (A14)

SororFago (A14)

Sunhee (A14)

Anaysia (A15)

Anon (A15)

Battleship (A15)

Boohoo (A15)

DekHockey33 (A15)

Epsocamisio (A15)

JSwag (A15)

KatherineG (A15)

LastResort (A15)

Looper (A15)

MinecraftSteve (A15)

Nebulosus (A15)

Nedarya (A15)

Oofda (A15)

ReMo (A15)

Remus (A15)

Rosalind (A15)

ShayRa (A15)

Soups (A15)

Strosahl (A15)

Switzerland (A15)

Waits (A15)

EagleEye (A16)

Lucyedi (A16)

PainterBoy (A16)

40AC (A17)

MyraDee (A18)

Kimona (A19)

20ES (A2)

AbbyPaige (A2)

Acolyte (A2)

Adzzy (A2)

Agape74 (A2)

AN3 (A2)

AN9 (A2)

ANI8 (A2)

AnnaL29 (A2)

Anselm (A2)

ArcherNM (A2)

BabyBack (A2)

Bactobuster (A2)

Baehexic (A2)

BengiVuitton (A2)

Benvolio (A2)

BiancaTri92 (A2)

BobSwaget (A2)

Bradman (A2)

Bugsy (A2)

Caraxes (A2)

Centaur (A2)

Changeling (A2)

Che12 (A2)

ChipMunk (A2)

Colin (A2)

CRB1 (A2)

Crucio (A2)

D29 (A2)

D32 (A2)

Dalmatian (A2)

DBQu4n (A2)

Deloris (A2)

Dignity (A2)

Drake55 (A2)

DudeLittle (A2)

Duplo (A2)

Echild (A2)

Equemioh13 (A2)

EvilGenius (A2)

EZMoney23 (A2)

Fameo (A2)

FiringLine (A2)

First (A2)

Flare16 (A2)

GaugeLDP (A2)

Georgie2 (A2)

HarryHoudini (A2)

Heffalump (A2)

Herbertwm (A2)

IronMan (A2)

Iwokeuplikedis (A2)

Jaan (A2)

Jerm (A2)

JoshKayV (A2)

Journey13 (A2)

Jsquared (A2)

Kalpine (A2)

Kerberos (A2)

KingCyrus (A2)

Koduck (A2)

L5 (A2)

LadyBird (A2)

Landor (A2)

Larenn (A2)

Leogania (A2)

Leviathan (A2)

LilTurb (A2)

Lokk (A2)

Loser (A2)

MajorMajor (A2)

Malec (A2)

MiculUcigas (A2)

Miko (A2)

MissWhite (A2)

Naji (A2)

NaSiaTalie (A2)

NicoleTera (A2)

NothingSpecial (A2)

Odin (A2)

Ph8s (A2)

Phaded (A2)

Piro94 (A2)

Pomar16 (A2)

Power (A2)

Pukovnik (A2)

QueenB2 (A2)

QueenBeesly (A2)

Quokka (A2)

Rachaly (A2)

RedRock (A2)

Retro23 (A2)

SemperFi (A2)

Serenity (A2)

Smeagan (A2)

SnapTap (A2)

StarStuff (A2)

Superchunk (A2)

SweetiePie (A2)

SwirlSquare (A2)

TarsusIV (A2)

TipsytheTRex (A2)

Tomathan (A2)

Travvers (A2)

Trixie (A2)

Trooper (A2)

Turbido (A2)

Updawg (A2)

VA6 (A2)

VC3 (A2)

WeiHuaDA (A2)

Whabigail7 (A2)

WideWale (A2)

XianYue (A2)

Anthony (A20)

Kinmap (A21)

ACFishhook (A3)

Aglet (A3)

AgronaGT15 (A3)

Anubis (A3)

AugsMagnumOpus (A3)

BabyJohn (A3)

BabyRay (A3)

Beauxregard13 (A3)

BlueBird (A3)

BreSam8 (A3)

Bugatti (A3)

BuzzBuzz (A3)

Bxz2 (A3)

Calvinny (A3)

Caviar (A3)

Chiqui (A3)

Colbster (A3)

Cullens (A3)

DaHudson (A3)

Daishi (A3)

Dieselweasel (A3)

DropBear (A3)

EpicPhail (A3)

Farber (A3)

Fernando (A3)

Fred313 (A3)

Gemma (A3)

GingkoMaracino (A3)

Giroux (A3)

Grif (A3)

Groupthink (A3)

Grub (A3)

Grum1 (A3)

GtownJaz (A3)

HashRod (A3)

HaveUMetTed (A3)

Heathen (A3)

HelDan (A3)

Heliosoles (A3)

Hercules11 (A3)

Hookmount (A3)

Idleandcovert (A3)

Isca (A3)

JenCasNa (A3)

JeppNRM (A3)

JF2 (A3)

JF4 (A3)

JHC117 (A3)

Jobu08 (A3)

Kachowdy (A3)

KADY (A3)

Kalb97 (A3)

Kalnoky (A3)

Lambert1 (A3)

LarryKay (A3)

LBerry (A3)

Lilith (A3)

Louie6 (A3)

LugYA (A3)

MA5 (A3)

MadMarie (A3)

Mainiac (A3)

Malinsilva (A3)

Manu (A3)

Marchesa (A3)

Margo (A3)

Marie (A3)

Marius (A3)

MarQuardt (A3)

Methuselah (A3)

Microwolf (A3)

Misomonster (A3)

MK4 (A3)

MoneyMay (A3)

MuchMore (A3)

Noella (A3)

Norbert (A3)

OlanP (A3)

Ollie (A3)

P28Green (A3)

Panamaxus (A3)

Pawn (A3)

Pembroke (A3)

Penny1 (A3)

PGHhamlin (A3)

Phantastic (A3)

PhishRPhriends (A3)

Phoebe (A3)

Phoxy (A3)

Phranny (A3)

Pistachio (A3)

Pocahontas (A3)

Popcicle (A3)

PotatoSplit (A3)

Puppy (A3)

PurpleHaze (A3)

QuinnKiro (A3)

Reba (A3)

ResDef (A3)

Rockstar (A3)

Rummer (A3)

Sabia (A3)

Sabinator (A3)

SaturnRing (A3)

Scout (A3)

Snickers (A3)

SoilDragon (A3)

Soshari (A3)

SoYo (A3)

Spike509 (A3)

Stagni (A3)

StepMih (A3)

Taurus (A3)

Texage (A3)

Tiffany (A3)

TNguyen7 (A3)

Todacoro (A3)

Veracruz (A3)

Vix (A3)

Watson (A3)

Wooldri (A3)

Zetzy (A3)

AbbysRanger (A4)

Abdiel (A4)

Achebe (A4)

Albee (A4)

Alberto7 (A4)

Annyong (A4)

Arturo (A4)

AvatarAhPeg (A4)

Avle17 (A4)

Baby16 (A4)

Backyardigan (A4)

Badger (A4)

BangNhom (A4)

Bartimeaus (A4)

BellusTerra (A4)

Blackmoor (A4)

Bombshell (A4)

Broseidon (A4)

Bruiser (A4)

BubbleTrouble (A4)

Bumblebee11 (A4)

Burger (A4)

Caelakin (A4)

Camperdownii (A4)

CentreCat (A4)

Cerulean (A4)

ChampagnePapi (A4)

Chaph (A4)

Cici (A4)

Cindaradix (A4)

Cintron (A4)

Citius (A4)

Clarenza (A4)

Cocoaberry (A4)

Commander (A4)

Connomayer (A4)

Datway (A4)

Deano (A4)

Dhanush (A4)

DirtyDunning (A4)

Druantia (A4)

Eagle (A4)

Eapen (A4)

Eris (A4)

Eros (A4)

Eurydice (A4)

Florean (A4)

Flux (A4)

Funston (A4)

Gadost (A4)

HamSlice (A4)

Happiness (A4)

Holli (A4)

Houdini22 (A4)

Huxley (A4)

Iceman (A4)

ICleared (A4)

Iracema64 (A4)

Jaykayelowell (A4)

JetBlade (A4)

JoongJeon (A4)

Kampy (A4)

Katalie136 (A4)

KFPoly (A4)

Kingmustik0402 (A4)

Koreni (A4)

Kratark (A4)

Kremtemulon (A4)

Kyee (A4)

LappelDuVide (A4)

Lemur (A4)

LeoAvram (A4)

LHTSCC (A4)

LittleB (A4)

LittleGuy (A4)

LochMonster (A4)

Lorenzo (A4)

Lunsford (A4)

Maverick (A4)

Maxo (A4)

Mazhar510 (A4)

Medusa (A4)

MeeZee (A4)

Melvin (A4)

Millski (A4)

Miramae (A4)

Morpher26 (A4)

Morrow (A4)

Mundrea (A4)

Nebs (A4)

Nemo27 (A4)

Noelle (A4)

NorthStar (A4)

NotAPhaseMom (A4)

Nyxis (A4)

Obama12 (A4)

Ohfah (A4)

OKaNui (A4)

Palestino (A4)

Peaches (A4)

Perplexer (A4)

PeterPeter (A4)

PetiteSangsue (A4)

Phacado (A4)

Phelipe (A4)

Phighter1804 (A4)

Phontbonne (A4)

Pipcraft (A4)

Polymorphads (A4)

Pumbaa (A4)

Relief (A4)

Romney (A4)

Roosevelt (A4)

Rosa24 (A4)

Ruin (A4)

Sabertooth (A4)

Scamp (A4)

Shaka (A4)

SheldonCooper (A4)

Shygu2 (A4)

Skipitt (A4)

Sparxx (A4)

Spino (A4)

Stasia (A4)

Stink (A4)

Taquarus (A4)

Thanksgivukkah (A4)

TinaFeyge (A4)

Tinybot (A4)

TiroTheta9 (A4)

TroyPia (A4)

TygerBlood (A4)

Ulysses (A4)

Wander (A4)

Wilbur (A4)

Wile (A4)

Wizard007 (A4)

Xena (A4)

YoSam321 (A4)

AgentM (A5)

Airmid (A5)

Aragog (A5)

Archetta (A5)

Benedict (A5)

Bluefalcon (A5)

Bonamassa (A5)

Chadwick (A5)

Conspiracy (A5)

Coog (A5)

Cuco (A5)

Discoknowium (A5)

Dublin (A5)

ElTiger69 (A5)

ForGetIt (A5)

George (A5)

Ghoulboy (A5)

HuhtaEnerson15 (A5)

Jabiru (A5)

Jovo (A5)

Lev2 (A5)

LittleCherry (A5)

MarysWell (A5)

Micasa (A5)

Midas2 (A5)

Milcery (A5)

Naca (A5)

PetterN (A5)

Phlorence (A5)

PickleBack (A5)

Scorpia (A5)

Swirley (A5)

SydNat (A5)

Tarynearal (A5)

Theia (A5)

Tiger (A5)

Twigg (A5)

UnionJack (A5)

Zolita (A5)

Artemis2UCLA (A6)

BABullseye (A6)

Blinn1 (A6)

Blue7 (A6)

Candra (A6)

Chartreuse (A6)

CloudWang3 (A6)

Cookiedough (A6)

DaVinci (A6)

Dorothea (A6)

EricB (A6)

Garak (A6)

Gladiator (A6)

GreedyLawyer (A6)

Gruunaga (A6)

Hammer (A6)

Helmet (A6)

Hexamo (A6)

Hoot (A6)

Indra (A6)

Isiphiwo (A6)

Jeffabunny (A6)

JewelBug (A6)

Jordennis (A6)

Kazan (A6)

Kipper29 (A6)

Koko (A6)

Lilbunny (A6)

McFly (A6)

Neeharika16 (A6)

Newrala (A6)

Pmask (A6)

Priamo (A6)

Rifter (A6)

Roksolana (A6)

SmellyB (A6)

SuperAwesome (A6)

SuperCallie99 (A6)

ToneTone (A6)

Tucker (A6)

VohminGhazi (A6)

Wiks (A6)

WunderPhul (A6)

Yokurt (A6)

Zaka (A6)

Zulu (A6)

DroogsArmy (A7)

FlyCatcher (A7)

HINdeR (A7)

Sheen (A7)

Timshel (A7)

Toro (A7)

Astro (A8)

Danforth (A8)

Dixon (A8)

Expelliarmus (A8)

Groundhog (A8)

NearlyHeadless (A8)

Phillis (A8)

Roary (A8)

Saintus (A8)

Smeadley (A8)

Stephig9 (A8)

Aliter (A9)

Alma (A9)

Arissanae (A9)

Beemo (A9)

BogosyJay (A9)

Catalina (A9)

Charm (A9)

Conquerage (A9)

Darrell (A9)

DreamTeam1 (A9)

EdogawaKiddo (A9)

Eidsmoe (A9)

Elephantoon (A9)

EmyBug (A9)

ExplosioNervosa (A9)

Fayely (A9)

Hanray (A9)

Holex (A9)

HortumSL17 (A9)

Jiawan (A9)

Keziacharles14 (A9)

Lilleskat (A9)

LoneWolf (A9)

Maminiaina (A9)

Myxus (A9)

Onglai (A9)

PackMan (A9)

Phaeder (A9)

Phonnegut (A9)

Pioneer (A9)

Priya (A9)

Qobbit (A9)

Rahalelujah (A9)

RyeScarlet (A9)

Sachima (A9)

Scherzo (A9)

SheaKeira (A9)

Spouty (A9)

Toaka (A9)

Tubs (A9)

Ugenie5 (A9)

Vanisoa (A9)

Yecey3 (A9)

Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
Plaque NotesLarge clear plaques average diameter of approximately 10.0 mm
MorphotypeSiphoviridae
Number of Genes97
Number of tRNAs0
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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Graduation_1

Graduation_2

Graduation_3

Graduation_4

Graduation_5

Graduation_6

Graduation_7

Graduation_8

Graduation_9

Graduation_10

Graduation_11
(lysin A)

Graduation_12
(lysin B)

Graduation_13
(terminase large subunit)

Graduation_14
(portal protein)

Graduation_15
(capsid maturation protease)

Graduation_16
(scaffolding protein)

Graduation_17
(capsid major subunit)

Graduation_18

Graduation_19
(head-to-tail adaptor)

Graduation_20
(head-to-tail stopper)

Graduation_21

Graduation_22
(tail terminator)

Graduation_23
(major tail protein)

Graduation_24
(tail assembly chaperone)

Graduation_25
(tail assembly chaperone)

Graduation_26
(tape measure protein)

Graduation_27
(minor tail protein)

Graduation_28

Graduation_29
(minor tail protein)

Graduation_30

Graduation_31

Graduation_32

Graduation_33
(minor tail protein)

Graduation_34
(carboxypeptidase)

Graduation_35

Graduation_36

Graduation_37

Graduation_38

Graduation_39
(integrase)

Graduation_40

Graduation_41

Graduation_42

Graduation_43

Graduation_44

Graduation_45

Graduation_46

Graduation_47
(DNA methyltransferase)

Graduation_48
(DNA polymerase I)

Graduation_49

Graduation_50

Graduation_51

Graduation_52

Graduation_53

Graduation_54

Graduation_55
(NrdC)

Graduation_56

Graduation_57

Graduation_58
(DNA primase)

Graduation_59
(DNA primase)

Graduation_60

Graduation_61

Graduation_62

Graduation_63
(EndoVII)

Graduation_64

Graduation_65

Graduation_66
(NrdH-like protein)

Graduation_67
(DnaB-like dsDNA helicase)

Graduation_68

Graduation_69

Graduation_70

Graduation_71

Graduation_72

Graduation_73
(helix-turn-helix DNA binding domain protein)

Graduation_74

Graduation_75
(Cas4 exonuclease)

Graduation_76

Graduation_77

Graduation_78
(immunity repressor)

Graduation_79

Graduation_80

Graduation_81

Graduation_82

Graduation_83

Graduation_84

Graduation_85

Graduation_86

Graduation_87

Graduation_88

Graduation_89
(DNA methyltransferase)

Graduation_90

Graduation_91

Graduation_92

Graduation_93

Graduation_94

Graduation_95

Graduation_96

Graduation_97

Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKF560331
Refseq NumberNC_022979
Archiving Info
Archiving status Archived
Pitt Freezer Box# 3
Pitt Freezer Box Grid# E1
Available Files
Plaque PictureDownload
EM PictureDownload
Final DNAMaster FileDownload