| Detailed Information for Phage Groundhog |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Nikole Kirk,Alexander Bradford,Gabriela Kline |
| Year Found | 2022 |
| Location Found | Roanoke, VA United States |
| Finding Institution | Virginia Western Community College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Unknown |
| Isolation Temperature | Not entered |
| GPS Coordinates | 37.31507 N, 80.01212 W Map |
| Discovery Notes | The soil that was collected was damp |
| Naming Notes | The reasoning for this name was because the soil we collected was from where multiple groundhogs had taken residence in my back yard |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 17, 2023 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 982 |
| Genome length (bp) | 52332 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGGATGTAA |
| GC Content | 61.3% |
| Sequencing Notes | The sample that was sequenced for this phage also had a low level (~10% of sequencing reads) of a Cluster A1 phage in it. |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | A |
| Subcluster | A8 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Virginia Western Community College |
| Annotation Status | In GenBank |
| Plaque Notes | The plaque is clearly noticeable but is somewhat cloudy.The plaques are a medium size |
| Morphotype | Siphoviridae |
| Number of Genes | 95 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | OR521068 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
153 |
| Pitt Freezer Box Grid# |
H11 |
| Available Files |
| GenBank File for Phamerator | Download |