| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Allyson King |
| Year Found | 2013 |
| Location Found | Bowling Green, KY USA |
| Finding Institution | Western Kentucky University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 36.988700 N, 86.452100 W Map |
| Discovery Notes | The collection site was approximately 10 feet from a campus building and approximately 4 feet away from a tree. There was a major road nearby the collection site and a sidewalk less than 10 feet away, therefore there was a lot of foot traffic near the sample site. The soil sample was dry, although there was sporadic rain in the area on the day the sample was taken. The sample was taken from a small ditch that had rotting acorns and leaves in it. |
| Naming Notes | The name that I chose is the Haitian Creole word for queen. I chose this name because whenever I introduce myself to people, they always comment on how my last name should be Queen instead of King. I chose to use Haitian Creole because my family and I help sponsor an orphanage in Haiti. Also Larenn has the same pronunciation as my sister's name, although her name is spelled differently. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 17, 2014 |
| Sequencing Facility | Virginia Commonwealth University Nucleic Acids Research Facilities |
| Shotgun Sequencing Method | 454 Pyrosequencing |
| Approximate Shotgun Coverage | 103 |
| Genome length (bp) | 52967 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGTCGGTTA |
| GC Content | 63.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | A |
| Subcluster | A2 |
| Cluster Life Cycle | Temperate |
| Lysogeny Notes | Because of the turbid plaques produced by this phage, it is suspected that Larenn is temperate. |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | The plaque morphology never differed from the time the enrichment was plated. The plaques are circles with a diameter of approximately 1.00 millimeter. They were extremely turbid and difficult to capture in pictures. The picture shown was created through side illumination and a very high contrast. Plaques formed after 22 hours of incubation at 37°C. |
| Morphotype | Siphoviridae |
| Number of Genes | 92 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KM677210 |
| Refseq Number | None yet |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info |
| Archiving status |
Archived |
| SEA Designator |
2013WEKULarennARK |
| SEA Lysate Titer |
8.1x10^9 |
| Date of SEA Lysate Titering |
Oct 14, 2013 |
| Pitt Freezer Box# |
5 |
| Pitt Freezer Box Grid# |
E12 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| XML File | Download |
