Mycobacterium phage Paraselene
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Skelbel (AZ1) Keough (EK2) MamaT (GI) Lilo27 (EB) Liberone (GI)Mycobacterium phage Paraselene
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| Detailed Information for Phage Paraselene | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Lyla Wood |
| Year Found | 2022 |
| Location Found | Brownsville, KY United States |
| Finding Institution | Western Kentucky University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 37.170112 N, 86.39894 W Map |
| Discovery Notes | My water sample was taken right off the bank where the Bear Creek and Green River converged. The water was full of organic material and sticks. |
| Naming Notes | Paraselene: A bright moonlike spot on a lunar halo; a mock moon. I named my phage Paraselene because the word refers to a halo-like phenomenon surrounding the moon and Paraselene's plaques have halos around the edges. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 20, 2023 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1830 |
| Genome length (bp) | 51176 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGATGGTAA |
| GC Content | 64.0% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | A |
| Subcluster | A1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members | |
| Annotating Institution | Western Kentucky University |
| Annotation Status | In GenBank |
| Plaque Notes | Paraselene phage plaques are turbid with halos around the edges. This indicates a lysogenic or temperate phage. The plaques are typically 5mm in size and the halos expand as time passes. |
| Morphotype | Siphoviridae |
| Number of Genes | 89 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List | |
| Submitted Minimal DNA Master File | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | OR253905 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 2.4 * 10^10 |
| Pitt Freezer Box# | 157 |
| Pitt Freezer Box Grid# | G10 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
