| Detailed Information for Phage Raid |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Abigail Yang |
| Year Found | 2024 |
| Location Found | Williamsburg, VA USA |
| Finding Institution | College of William & Mary |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 21°C |
| GPS Coordinates | 37.27159 N, 76.716536 W Map |
| Discovery Notes | I collected the soil sample from the bank of the Grim Dell, digging about an inch into the mud. The soil was very wet and gritty, filled with dead leaves. |
| Naming Notes | My phage is named Raid in honor of the cockroach killer spray. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 15, 2025 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NextSeq 1000 |
| Read Type | Single-end reads |
| Read Length | 100 bp |
| Approximate Shotgun Coverage | 7672 |
| Genome length (bp) | 51486 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGATGGTAA |
| GC Content | 63.8% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | A |
| Subcluster | A1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | College of William & Mary |
| Annotation Status | Overdue (Expected completion by 7/1/2025) |
| Plaque Notes | The plaque had huge bullseye morphologies at certain rounds of purification and circular plaques at other rounds. The plaques from earlier isolation rounds were smaller, about 1/4 inch in diameter. The plaques from later purification rounds were about 1/8 inch in diameter. The plaques from the last round were about 3/8 inch in diameter. |
| Morphotype | Siphoviridae |
| Number of Genes | 89 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
208 |
| Pitt Freezer Box Grid# |
H2 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
