| Detailed Information for Phage TarsusIV |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Laura Teal |
| Year Found | 2013 |
| Location Found | Holland, MI USA |
| Finding Institution | Hope College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 42.793889 N, 86.1089 W Map |
| Discovery Notes | Soil Depth: ~2cm.
Soil moisture content: pretty dry
Isolated at 30ºC incubation |
| Naming Notes | It's a reference to the Original Series of Star Trek — a planet that was subjected to a genocide that killed half the population. I thought the name appropriate for a temperate phage. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | May 13, 2020 |
| Sequencing Facility | Hope College |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 5962 |
| Genome length (bp) | 52704 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGTCGGTTA |
| GC Content | 63.0% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | A |
| Subcluster | A2 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Indian River State College |
| Annotation Status | In GenBank |
| Plaque Notes | Turbid plaque with an average size of 3.5mm, but with variation anywhere from 1mm to 6mm in diameter. Slightly clearer ring around the outside with a more turbid center. This morphology is more evident the lower the concentration of calcium chloride in the phage buffer and cell culture. |
| Morphotype | Siphoviridae |
| Number of Genes | 92 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MW601216 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
3.8x10^10 |
| Pitt Freezer Box# |
5 |
| Pitt Freezer Box Grid# |
C4 |
| Available Files |
| GenBank File for Phamerator | Download |