| Detailed Information for Phage Zeuska |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Emma Herold |
| Year Found | 2013 |
| Location Found | Providence, RI USA |
| Finding Institution | Brown University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 41.824047 N, 71.403114 W Map |
| Discovery Notes | Sample obtained from cool soil lying under a tanbark top layer, all found underneath an overhanging tree. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 18, 2013 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 3844 |
| Genome length (bp) | 53598 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGATGGTAA |
| GC Content | 63.7% |
| Sequencing Notes | Some of the reads from this project indicate that a portion of the genome has been deleted in a subset of the population. About 15% of the reads show this deletion. The deletion is 1803 bp and runs from position 26961 to 28763 (inclusive). There is a 12 bp sequence (CGGGCTGCTGGG) located near these two positions that is collapsed into one copy in the deletion version of the genome. |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | A |
| Subcluster | A1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Tarleton State University |
| Annotation Status | In GenBank |
| Plaque Notes | Predicted cluster type: D, F1, or F2 |
| Morphotype | Siphoviridae |
| Number of Genes | 93 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK524506 |
| Refseq Number | None yet |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1.1x10^10 |
| Pitt Freezer Box# |
4 |
| Pitt Freezer Box Grid# |
E11 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
