| Detailed Information for Phage Jabiru |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Joe Skibbens |
| Year Found | 2014 |
| Location Found | Palo Verde, Costa Rica |
| Finding Institution | Lehigh University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Unknown |
| Isolation Temperature | Not entered |
| GPS Coordinates | 10.4 N, 85.32 W Map |
| Discovery Notes | This sample was taken from a vast wetlands environment. The soil had been recently exposed by the water receding and was loamy and moist. The wetland was brimming with flora and fauna, probably an indicator of rich soil. |
| Naming Notes | Named after the Jabiru stork, indigenous to the area in which this phage was found. The Jabiru is one of the largest birds in Central and South America. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jul 10, 2014 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Ion Torrent |
| Approximate Shotgun Coverage | 2707 |
| Genome length (bp) | 50530 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGGAGGTAA |
| GC Content | 60.0% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | A |
| Subcluster | A5 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Lehigh University |
| Annotation Status | In GenBank |
| Plaque Notes | Jabiru creates plaques about 2mm in diameter after 24 hours (closer to 4mm after 48 hours). The plaques are turbid with a bulls-eye clearing in the center (about 1mm in diameter). Typically as the plaque expands the turbid ring grows outward while the clear center remains the same size.
It should be noted that certain factors (potentially the thickness of its host's bacterial lawn) can cause it to form clear plaques of the same size. |
| Morphotype | Siphoviridae |
| Number of Genes | 86 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH051254 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1.11x10^11 |
| Date of SEA Lysate Titering |
Mar 11, 2014 |
| Pitt Freezer Box# |
7 |
| Pitt Freezer Box Grid# |
A7 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
