Mycobacterium phage Alexphander
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Detailed Information for Phage Alexphander
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByDaniel Shen
Year Found2016
Location FoundWestwood, CA USA
Finding InstitutionUniversity of California, Los Angeles
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature30°C
GPS Coordinates34.075071 N, 118.450961 W Map
Discovery NotesPhage Alexphander was isolated from soil taken from compost heap in the upper garden of the UCLA recreation center. Phage was isolated via direct plating at 30°C.

Initial characterization performed by Daniel Shen. Annotation performed by Team PhagentX (Annie Odelson, April Johnson, Elizabeth Vanderwall, Kevin Ha) and Team The Life of Phablo (Michael Murphy, Parker Sevier, Darwin Wu.)

Name of individual who collected sample: Daniel Shen

Instructor: Dr. Jordan Moberg Parker
Teaching Assistant: Michelle Bina
Undergraduate Assistant: Sam Wu
Laboratory Support: Dr. Krisanavane Reddi
Naming NotesThis phage was from the same soil sample as phage Familton.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedDec 21, 2016
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Genome length (bp)57734
Character of genome endsUnknown
Overhang Length10 bases
Overhang SequenceCCGATGGCAT
GC Content61.2%
Fasta file available?Yes: Download fasta file
Characterization
ClusterF
SubclusterF1
Cluster Life CycleTemperate
Other Cluster Members

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Blarby (None)

MulchSalad (None)

Akhila (F1)

Alexphander (F1)

AlpineSix (F1)

ArcusAngelus (F1)

Ardmore (F1)

Aubs (F1)

Awesomesauce (F1)

Batiatus (F1)

Beakin (F1)

BigPhil (F1)

Bipolar (F1)

Blexus (F1)

BlueCrab (F1)

BobaPhett (F1)

Bobi (F1)

BodEinwohner17 (F1)

Boomer (F1)

Brocalys (F1)

Bubbles123 (F1)

Burwell21 (F1)

BuzzLyseyear (F1)

ByChance (F1)

Byougenkin (F1)

Cabrinians (F1)

CaptainTrips (F1)

Cerasum (F1)

Che8 (F1)

Chevrolet (F1)

ChickenDinner (F1)

Chuckly (F1)

Clifton (F1)

Coco12 (F1)

Cornucopia (F1)

Crumble (F1)

DaddyRickover (F1)

Daenerys (F1)

Dante (F1)

DaWorst (F1)

DeadP (F1)

Deb65 (F1)

DillTech15 (F1)

DirtMcgirt (F1)

DLane (F1)

DocMcStuffins (F1)

Donkeykong (F1)

Doomphist (F1)

Dorothy (F1)

DosHalletts (F1)

DotProduct (F1)

Doug (F1)

Drago (F1)

DRBy19 (F1)

Eish (F1)

EleanorGeorge (F1)

Emma (F1)

Empress (F1)

Enby (F1)

Eradicator (F1)

Estave1 (F1)

Fancypants (F1)

Fastidio (F1)

Filuzino (F1)

Firehouse51 (F1)

Flathead (F1)

Florinda (F1)

Frankie (F1)

FreddyB (F1)

Fruitloop (F1)

Galactic (F1)

Gandalph (F1)

Geralt (F1)

GigiOuiOui (F1)

Girafales (F1)

Girr (F1)

Gorge (F1)

GUmbie (F1)

Guppsters (F1)

Hades (F1)

Hamulus (F1)

Harley (F1)

Hegedechwinu (F1)

Hlubikazi (F1)

Ibhubesi (F1)

IbOuu (F1)

IMpilo (F1)

Inventum (F1)

IrishSherpFalk (F1)

JalFarm20 (F1)

James (F1)

Jarcob (F1)

Jinglebell (F1)

Job42 (F1)

JoeyJr (F1)

Juice456 (F1)

Juniper1 (F1)

Karhdo (F1)

Kenuha5 (F1)

Kersh (F1)

Kimberlium (F1)

KingMidas (F1)

Kingsley (F1)

Koella (F1)

Krakatau (F1)

KristaRAM (F1)

LastJedi (F1)

Latretium (F1)

Leozinho (F1)

Leperchaun (F1)

LilMoolah (F1)

LittleShirley (F1)

Lizziana (F1)

Llama (F1)

Llij (F1)

Lorde (F1)

LunaBlu (F1)

Madiba (F1)

MadMen (F1)

Mahavrat (F1)

Mandlovu (F1)

Mantra (F1)

Maravista (F1)

Marker (F1)

Martik (F1)

Mattes (F1)

Melissauren88 (F1)

MilanaBonita (F1)

MilleniumForce (F1)

MinionDave (F1)

Minnie (F1)

Misha28 (F1)

MisterCuddles (F1)

Modragons (F1)

Moonbeam (F1)

Mova (F1)

Mozy (F1)

Ms6 (F1)

MulchExplorer (F1)

Mutaforma13 (F1)

Nimbo (F1)

Nitzel (F1)

Nivrat (F1)

NormanBulbieJr (F1)

Ochi17 (F1)

OfUltron (F1)

Ogopogo (F1)

Oksu (F1)

OldBen (F1)

OlympiaSaint (F1)

Ovechkin (F1)

OwlsT2W (F1)

Pacc40 (F1)

Phalconet (F1)

Phanphagia (F1)

PHappiness (F1)

Phasih (F1)

Phatniss (F1)

PhesterPhotato (F1)

Philus (F1)

Piper2020 (F1)

Pippy (F1)

Plumbus (F1)

PMC (F1)

Poenanya (F1)

Polka14 (F1)

Pollywog (F1)

PopTart (F1)

Priscilla (F1)

QuickMath (F1)

Quico (F1)

Radiance (F1)

Ramsey (F1)

RedBird (F1)

Rialto (F1)

Rita (F1)

RitaG (F1)

Rockne (F1)

RockyHorror (F1)

Royals2015 (F1)

Ruby (F1)

Saal (F1)

Sabbb (F1)

Sandalphon (F1)

Sarma624 (F1)

Sassafras (F1)

SassyB (F1)

Scottish (F1)

Seabastian (F1)

Seagreen (F1)

SG4 (F1)

Shauna1 (F1)

ShiLan (F1)

ShowerHandel (F1)

ShroomBoi (F1)

SimranZ1 (F1)

SiSi (F1)

Sparkdehlily (F1)

Spartacus (F1)

Spikelee (F1)

Spoonbill (F1)

StAnnes (F1)

Stap (F1)

Starcevich (F1)

Strokeseat (F1)

SuperGrey (F1)

SwagPigglett (F1)

Taj (F1)

TDanisky (F1)

TootsiePop (F1)

Tootsieroll (F1)

Totinger (F1)

Tweety (F1)

UncleRicky (F1)

Velveteen (F1)

Veteran (F1)

VRedHorse (F1)

Wachhund (F1)

Wee (F1)

Whatsapiecost (F1)

Whouxphf (F1)

WillSterrel (F1)

XFactor (F1)

Yorick (F1)

Zerg (F1)

Zizzle (F1)

Avani (F2)

Che9d (F2)

Demsculpinboyz (F2)

Jabbawokkie (F2)

Soul22 (F2)

Yoshi (F2)

Zapner (F2)

Squirty (F3)

LunaStella (F4)

Renaud18 (F4)

TChen (F4)

ThetaBob (F4)

Cornie (F5)

Toron (F6)

Annotating InstitutionUniversity of California, Los Angeles
Annotation StatusIn GenBank
Plaque NotesPinpoint, 1 mm
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Alexphander_1
(HNH endonuclease)

Alexphander_2
(terminase small subunit)

Alexphander_3
(terminase large subunit)

Alexphander_4
(portal protein)

Alexphander_5
(capsid maturation protease)

Alexphander_6
(scaffolding protein)

Alexphander_7
(major capsid protein)

Alexphander_8
(head-to-tail adaptor)

Alexphander_9
(head-to-tail stopper)

Alexphander_10

Alexphander_11
(tail terminator)

Alexphander_12
(major tail protein)

Alexphander_13
(tail assembly chaperone)

Alexphander_14
(tail assembly chaperone)

Alexphander_15
(HNH endonuclease)

Alexphander_16
(tape measure protein)

Alexphander_17
(minor tail protein)

Alexphander_18
(minor tail protein)

Alexphander_19
(minor tail protein)

Alexphander_20
(minor tail protein)

Alexphander_21
(minor tail protein)

Alexphander_22
(minor tail protein)

Alexphander_23
(minor tail protein)

Alexphander_24

Alexphander_25
(minor tail protein)

Alexphander_26
(minor tail protein)

Alexphander_27
(helix-turn-helix DNA binding domain protein)

Alexphander_28

Alexphander_29

Alexphander_30

Alexphander_31

Alexphander_32

Alexphander_33
(lysin A)

Alexphander_34
(lysin B)

Alexphander_35
(holin)

Alexphander_36

Alexphander_37

Alexphander_38
(DnaQ-like DNA polymerase III subunit)

Alexphander_39

Alexphander_40

Alexphander_41

Alexphander_42

Alexphander_43

Alexphander_44

Alexphander_45
(integrase)

Alexphander_46
(RelE-like toxin)

Alexphander_47
(RelB-like antitoxin)

Alexphander_48

Alexphander_49

Alexphander_50
(immunity repressor)

Alexphander_51
(CRO protein)

Alexphander_52
(antirepressor)

Alexphander_53

Alexphander_54

Alexphander_55
(excise)

Alexphander_56

Alexphander_57

Alexphander_58

Alexphander_59

Alexphander_60

Alexphander_61
(WhiB family transcription factor)

Alexphander_62

Alexphander_63
(WhiB family transcription factor)

Alexphander_64
(helix-turn-helix DNA binding domain protein)

Alexphander_65

Alexphander_66

Alexphander_67

Alexphander_68

Alexphander_69
(HNH endonuclease)

Alexphander_70
(DNA helicase)

Alexphander_71

Alexphander_72

Alexphander_73

Alexphander_74

Alexphander_75

Alexphander_76

Alexphander_77

Alexphander_78

Alexphander_79

Alexphander_80
(MPME1 protein)

Alexphander_81

Alexphander_82

Alexphander_83

Alexphander_84

Alexphander_85

Alexphander_86

Alexphander_87

Alexphander_88

Alexphander_89

Alexphander_90

Alexphander_91

Alexphander_92

Alexphander_93

Alexphander_94
(MerR-like HTH DNA binding protein)

Alexphander_95

Alexphander_96

Alexphander_97

Alexphander_98

Alexphander_99

Alexphander_100

Alexphander_101

Alexphander_102
(glycosyltransferase)

Alexphander_103

Alexphander_104
(glycosyltransferase)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMG962361
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 58
Pitt Freezer Box Grid# F3
Available Files
Plaque PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload