The Actinobacteriophage Database

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Detailed Information for Phage Emma
Discovery Information
Isolation Host	Mycobacterium smegmatis mc²155
Found By	Teams Foster the Phages and 4 Man Wolf Plaque
Year Found	2015
Location Found	Los Angeles, CA USA
Finding Institution	University of California, Los Angeles
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	Yes
Isolation Temperature	34°C
GPS Coordinates	34.06 N, 118.45 W Map
Discovery Notes	Soil sample was collected by Daniela Bradvica from base of a mint plant in the backyard of a residence near UCLA from a depth of 1cm. Phage was isolated,characterized, and annotated by Team Foster the Phage and Team Four Man Wolf Plaque. Foster the Phage: Alison Abele, Cynthia Araradian, Julia Lee, Brian Rodriguez Four Man Wolf Plaque: Daniela Bradvica, Wyatt Nelson, Samantha Santopoalo, Lauren Estiandan TA: Emma Goodwin Instructor: Jordan Moberg Parker
Naming Notes	Named after the Teaching Assistant for this section.
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Sep 11, 2015
Sequencing Facility	NC State Genomic Sciences Laboratory
Shotgun Sequencing Method	Illumina Sequencing
Approximate Shotgun Coverage	5610
Genome length (bp)	56418
Character of genome ends	3' Sticky Overhang
Overhang Length	10 bases
Overhang Sequence	CGGAAGGCGC
GC Content	61.3%
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	F
Subcluster	F1
Cluster Life Cycle	Temperate
Lysogeny Notes	Phage Emma was capable of lysogeny. Plaques exhibited classic bulls-eye morphology. Annotation revealed lysogeny cassette with integrase (Y-int), immunity repressors, and CRO.
Other Cluster Members	Click to View AbbyNormal (None) Grib (None) IchabodCrane (None) Kishmeesh (None) KitCat (None) MightyMorph (None) Puck (None) Renaud18 (None) Sargento (None) WIVsmall1220 (None) Alexphander (F1) Anaxagorus (F1) AndrewsK (F1) Ant1Dodson (F1) ArcusAngelus (F1) Ardmore (F1) Arienne (F1) Asmart (F1) Awesomesauce (F1) BabytheFifth (F1) Barson (F1) Batiatus (F1) BigPhil (F1) Bipolar (F1) Blexus (F1) BobaPhett (F1) Bobi (F1) BodEinwohner17 (F1) BoomCrackaPop (F1) Boomer (F1) Braemar (F1) Brocalys (F1) Bubbles123 (F1) Burwell21 (F1) BuzzLyseyear (F1) ByChance (F1) Byougenkin (F1) Cabrinians (F1) Cakepop (F1) CaptainTrips (F1) Cerasum (F1) Che8 (F1) Chhibber (F1) Chikondi (F1) Chuckly (F1) Clifton (F1) Clover (F1) CoolGuy (F1) Cornucopia (F1) Daenerys (F1) Danda (F1) Dante (F1) DaWorst (F1) DCrust15 (F1) DeadP (F1) Desmond (F1) DillTech15 (F1) DirtMcgirt (F1) DLane (F1) Docouto (F1) Dorothy (F1) DotProduct (F1) Doug (F1) Drago (F1) DRBy19 (F1) Eish (F1) EleanorGeorge (F1) Emma (F1) Empress (F1) EmWilla (F1) Enby (F1) Estave1 (F1) Estella (F1) Fancypants (F1) Filuzino (F1) Firehouse51 (F1) Flathead (F1) Florinda (F1) Frankie (F1) Freddie4 (F1) Fruitloop (F1) FunOne (F1) Galactic (F1) Gardregs (F1) Gendisarray (F1) Genesis (F1) Geralt (F1) Girafales (F1) Girr (F1) Gorge (F1) GreatPumpkin (F1) GUmbie (F1) Hades (F1) Hamulus (F1) Harley (F1) Hegedechwinu (F1) Ibhubesi (F1) Inventum (F1) IrishSherpFalk (F1) James (F1) Job42 (F1) JoeyJr (F1) Judylove (F1) Juice456 (F1) Kalikimaka (F1) Katya (F1) Kenuha5 (F1) Kersh (F1) Keyes (F1) Kimberlium (F1) KingMidas (F1) Kingsley (F1) Koella (F1) Krakatau (F1) KristaRAM (F1) Latretium (F1) LillyLatzon (F1) LilMoolah (F1) Lizziana (F1) Llama (F1) Llij (F1) Lorde (F1) Lysinator (F1) MacDrage (F1) Mahavrat (F1) Mantra (F1) Mattes (F1) McDiddy (F1) Melissauren88 (F1) MerrillCO18 (F1) Mille (F1) MilleniumForce (F1) MinionDave (F1) Minnie (F1) Misha28 (F1) MisterCuddles (F1) Modragons (F1) Mozy (F1) Ms6 (F1) Mutaforma13 (F1) Myosotis (F1) Nemain (F1) Nimbo (F1) Nitzel (F1) Nivrat (F1) Noodle (F1) NormanBulbieJr (F1) Ochi17 (F1) OfUltron (F1) Ogopogo (F1) OldBen (F1) OlympiaSaint (F1) Ovechkin (F1) OwlsT2W (F1) Pacc40 (F1) Peanut21s (F1) Phabio (F1) PHappiness (F1) Phasih (F1) Phatniss (F1) PhrankthaTank (F1) Piper2020 (F1) Pippy (F1) Plackberry (F1) Plumbus (F1) PMC (F1) Poenanya (F1) Polka14 (F1) Pollywog (F1) PopTart (F1) Priscilla (F1) Prometheus (F1) QuickMath (F1) Quico (F1) Ramsey (F1) RitaG (F1) Rocktrap (F1) RockyHorror (F1) Rombolo (F1) Royals2015 (F1) Ruby (F1) S2Kari (F1) S2TSquared (F1) Saal (F1) SaltyMike (F1) Sandalphon (F1) SassyB (F1) Scottish (F1) Seagreen (F1) SG4 (F1) Sharpshooter (F1) Shauna1 (F1) SherL (F1) ShiLan (F1) SimranZ1 (F1) SiSi (F1) Sparkdehlily (F1) Spartacus (F1) Spikelee (F1) Spoonbill (F1) StAnnes (F1) Strokeseat (F1) SuperGrey (F1) SwagPigglett (F1) Taj (F1) Taus (F1) TDanisky (F1) TonaldTDrump (F1) TootsiePop (F1) Tweety (F1) UncleRicky (F1) Velveteen (F1) Wachhund (F1) Wee (F1) WheatBerry18 (F1) Whouxphf (F1) WillSterrel (F1) WIVsmall (F1) XFactor (F1) Zerg (F1) Avani (F2) Che9d (F2) Demsculpinboyz (F2) Jabbawokkie (F2) Yoshi (F2) Zapner (F2) Squirty (F3) TChen (F4) ThetaBob (F4) Cornie (F5)
Annotation Status	In GenBank
Plaque Notes	Two morphologies: -Clear plaques, 3 mm diameter -Bull’s eye plaques, clear center, surrounding turbid region, clear ring on outside, 3 mm diameter
Morphotype	Siphoviridae
Number of Genes	108
Has been Phamerated?	Yes
Gene List	Click to View Emma_1 (terminase small subunit) Emma_2 (terminase large subunit) Emma_3 (portal protein) Emma_4 (capsid maturation protease) Emma_5 (scaffolding protein) Emma_6 (major capsid protein) Emma_7 (head-to-tail connector protein) Emma_8 (head-to-tail connector protein) Emma_9 (head-to-tail connector protein) Emma_10 (head-to-tail connector protein) Emma_11 (major tail protein) Emma_12 (tail assembly chaperone) Emma_13 (tail assembly chaperone) Emma_14 (tape measure protein) Emma_15 (minor tail protein) Emma_16 (minor tail protein) Emma_17 (minor tail protein) Emma_18 (minor tail protein) Emma_19 (minor tail protein) Emma_20 (minor tail protein) Emma_21 (minor tail protein) Emma_22 (minor tail protein) Emma_23 (minor tail protein) Emma_24 (minor tail protein) Emma_25 (helix-turn-helix DNA binding domain protein) Emma_26 Emma_27 Emma_28 Emma_29 Emma_30 Emma_31 Emma_32 (lysin A) Emma_33 (lysin B) Emma_34 (holin) Emma_35 (putative membrane protein) Emma_36 Emma_37 (DNA polymerase III subunit) Emma_38 Emma_39 Emma_40 (putative membrane protein) Emma_41 (immunity repressor) Emma_42 Emma_43 Emma_44 (integrase) Emma_45 Emma_46 (immunity repressor) Emma_47 (CRO protein) Emma_48 Emma_49 Emma_50 Emma_51 Emma_52 Emma_53 Emma_54 Emma_55 Emma_56 Emma_57 Emma_58 Emma_59 (WhiB family transcription factor) Emma_60 Emma_61 (helix-turn-helix DNA binding domain protein) Emma_62 Emma_63 Emma_64 Emma_65 Emma_66 (AAA ATPase) Emma_67 (HNH endonuclease) Emma_68 (DNA helicase) Emma_69 (DNA methylase) Emma_70 Emma_71 Emma_72 Emma_73 Emma_74 Emma_75 Emma_76 (HTH DNA binding protein) Emma_77 Emma_78 Emma_79 Emma_80 Emma_81 Emma_82 Emma_83 Emma_84 Emma_85 Emma_86 Emma_87 (MPME protein) Emma_88 Emma_89 Emma_90 Emma_91 Emma_92 Emma_93 Emma_94 Emma_95 Emma_96 Emma_97 Emma_98 Emma_99 Emma_100 Emma_101 Emma_102 Emma_103 Emma_104 Emma_105 Emma_106 (glycosyltransferase) Emma_107 Emma_108 (glycosyltransferase) Emma_109 (HNH endonuclease)
Submitted Annotation in DNA Master Format	Download
Submitted Minimal DNA Master File	Download
Author List	Download
Annotation Cover Sheet	Download
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	MF668270
Refseq Number	None yet
Archiving Info
Archiving status	Archived
Pitt Freezer Box#	23
Pitt Freezer Box Grid#	E4
Available Files
Plaque Picture	Download
Restriction Digest Picture	Download
EM Picture	Download
GenBank File for Phamerator	Download

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