Mycobacterium phage BodEinwohner17
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Detailed Information for Phage BodEinwohner17
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Former namesBodeinwohner17
Found BySeth Donnelley
Year Found2017
Location FoundLincoln University, PA USA
Finding InstitutionLincoln University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature19°C
GPS Coordinates39.804235 N, 75.924834 W Map
Discovery NotesI isolated this phage from a soil sample collected in a relatively low area on the Lincoln University campus, in the soil under a fir tree. The soil under the tree had only a small amount of moss on the surface, but was still quite moist due to the somewhat high humidity and shaded conditions under the tree. The temperature of the surrounding air was ~19*C, and the sky was mostly sunny.
Naming NotesA contraction of "boden" (meaning "soil") and "einwohner" (meaning "inhabitant" or "resident"). These two German words form a contraction which describes the origin of this bacteriophages. The fact that they are from German refers to the fact that Mycobacterium smegmatis, the host bacterium, was first isolated by Lustgarten. "17" refers to the year in which it was isolated.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 12, 2018
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1902
Genome length (bp)60222
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGACGGCGC
GC Content61.3%
Fasta file available?Yes: Download fasta file
Characterization
ClusterF
SubclusterF1
Cluster Life CycleTemperate
Other Cluster Members

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Soul22 (None)

Akhila (F1)

Alexphander (F1)

ArcusAngelus (F1)

Ardmore (F1)

Awesomesauce (F1)

Batiatus (F1)

BigPhil (F1)

Bipolar (F1)

Blexus (F1)

BobaPhett (F1)

Bobi (F1)

BodEinwohner17 (F1)

Boomer (F1)

Brocalys (F1)

Bubbles123 (F1)

Burwell21 (F1)

BuzzLyseyear (F1)

ByChance (F1)

Byougenkin (F1)

Cabrinians (F1)

CaptainTrips (F1)

Cerasum (F1)

Che8 (F1)

Chuckly (F1)

Clifton (F1)

Coco12 (F1)

Cornucopia (F1)

Daenerys (F1)

Dante (F1)

DaWorst (F1)

DeadP (F1)

DillTech15 (F1)

DirtMcgirt (F1)

DLane (F1)

Donkeykong (F1)

Doomphist (F1)

Dorothy (F1)

DotProduct (F1)

Doug (F1)

Drago (F1)

DRBy19 (F1)

Eish (F1)

EleanorGeorge (F1)

Emma (F1)

Empress (F1)

Enby (F1)

Estave1 (F1)

Fancypants (F1)

Filuzino (F1)

Firehouse51 (F1)

Flathead (F1)

Florinda (F1)

Frankie (F1)

Fruitloop (F1)

Galactic (F1)

Gandalph (F1)

Geralt (F1)

Girafales (F1)

Girr (F1)

Gorge (F1)

GUmbie (F1)

Hades (F1)

Hamulus (F1)

Harley (F1)

Hegedechwinu (F1)

Hlubikazi (F1)

Ibhubesi (F1)

Inventum (F1)

IrishSherpFalk (F1)

James (F1)

Job42 (F1)

JoeyJr (F1)

Juice456 (F1)

Kenuha5 (F1)

Kersh (F1)

Kimberlium (F1)

KingMidas (F1)

Kingsley (F1)

Koella (F1)

Krakatau (F1)

KristaRAM (F1)

LastJedi (F1)

Latretium (F1)

LilMoolah (F1)

Lizziana (F1)

Llama (F1)

Llij (F1)

Lorde (F1)

Mahavrat (F1)

Mandlovu (F1)

Mantra (F1)

Martik (F1)

Mattes (F1)

Melissauren88 (F1)

MilleniumForce (F1)

MinionDave (F1)

Minnie (F1)

Misha28 (F1)

MisterCuddles (F1)

Modragons (F1)

Moonbeam (F1)

Mova (F1)

Mozy (F1)

Mutaforma13 (F1)

Nimbo (F1)

Nitzel (F1)

Nivrat (F1)

NormanBulbieJr (F1)

Ochi17 (F1)

OfUltron (F1)

Ogopogo (F1)

OldBen (F1)

OlympiaSaint (F1)

Ovechkin (F1)

OwlsT2W (F1)

Pacc40 (F1)

Phanphagia (F1)

PHappiness (F1)

Phasih (F1)

Phatniss (F1)

Piper2020 (F1)

Pippy (F1)

Plumbus (F1)

PMC (F1)

Poenanya (F1)

Polka14 (F1)

Pollywog (F1)

PopTart (F1)

Priscilla (F1)

QuickMath (F1)

Quico (F1)

Ramsey (F1)

RitaG (F1)

RockyHorror (F1)

Royals2015 (F1)

Ruby (F1)

Saal (F1)

Sandalphon (F1)

Sassafras (F1)

SassyB (F1)

Scottish (F1)

Seabastian (F1)

Seagreen (F1)

SG4 (F1)

Shauna1 (F1)

ShiLan (F1)

SimranZ1 (F1)

SiSi (F1)

Sparkdehlily (F1)

Spartacus (F1)

Spikelee (F1)

Spoonbill (F1)

StAnnes (F1)

Strokeseat (F1)

SuperGrey (F1)

SwagPigglett (F1)

Taj (F1)

TDanisky (F1)

TootsiePop (F1)

Tweety (F1)

UncleRicky (F1)

Velveteen (F1)

Veteran (F1)

Wachhund (F1)

Wee (F1)

Whouxphf (F1)

WillSterrel (F1)

XFactor (F1)

Zerg (F1)

Avani (F2)

Che9d (F2)

Demsculpinboyz (F2)

Jabbawokkie (F2)

Yoshi (F2)

Zapner (F2)

Squirty (F3)

Renaud18 (F4)

TChen (F4)

ThetaBob (F4)

Cornie (F5)

Annotating InstitutionUniversity of Pittsburgh
Annotation StatusIn GenBank
Plaque NotesThe mean diameter of the plaques formed by this phage is ~2.6mm. Most of them have a smaller circle with a diameter of ~1.0mm in the center which is completely clear. Outside of this, there is a band of cloudy material, which starts near its inside border as being just as defined as the bacterial lawn on the rest of the plate outside the plaque, but which becomes almost completely clear towards the outside of the plaque. Here, a cleared ring is formed, sometimes directionally, with respect to the plaque as a whole. Additionally, there appears to be a lighter "halo" under the surface of the bacterial lawn/top agar for some of the plaques, which extends out ~2.0mm from each plaque. This could be caused by the infiltration of air under the surface of the top agar where the clearings had exposed this area to the air.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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BodEinwohner17_1
(terminase small subunit)

BodEinwohner17_2
(terminase large subunit)

BodEinwohner17_3
(portal protein)

BodEinwohner17_4
(MuF-like minor capsid protein)

BodEinwohner17_5
(scaffolding protein)

BodEinwohner17_6
(major capsid protein)

BodEinwohner17_7
(head-to-tail adaptor)

BodEinwohner17_8
(head-to-tail stopper)

BodEinwohner17_9

BodEinwohner17_10
(tail terminator)

BodEinwohner17_11
(major tail protein)

BodEinwohner17_12
(tail assembly chaperone)

BodEinwohner17_13
(tail assembly chaperone)

BodEinwohner17_14
(tape measure protein)

BodEinwohner17_15
(minor tail protein)

BodEinwohner17_16
(minor tail protein)

BodEinwohner17_17
(minor tail protein)

BodEinwohner17_18
(minor tail protein)

BodEinwohner17_19
(minor tail protein)

BodEinwohner17_20
(minor tail protein)

BodEinwohner17_21

BodEinwohner17_22

BodEinwohner17_23

BodEinwohner17_24

BodEinwohner17_25
(helix-turn-helix DNA binding protein)

BodEinwohner17_26

BodEinwohner17_27

BodEinwohner17_28

BodEinwohner17_29

BodEinwohner17_30
(lysin A)

BodEinwohner17_31
(lysin B)

BodEinwohner17_32
(holin)

BodEinwohner17_33

BodEinwohner17_34

BodEinwohner17_35
(DnaQ-like exonuclease)

BodEinwohner17_36

BodEinwohner17_37

BodEinwohner17_38

BodEinwohner17_39

BodEinwohner17_40

BodEinwohner17_41
(tyrosine integrase)

BodEinwohner17_42

BodEinwohner17_43

BodEinwohner17_44
(immunity repressor)

BodEinwohner17_45
(Cro protein)

BodEinwohner17_46

BodEinwohner17_47
(immunity repressor)

BodEinwohner17_48

BodEinwohner17_49

BodEinwohner17_50

BodEinwohner17_51

BodEinwohner17_52

BodEinwohner17_53

BodEinwohner17_54

BodEinwohner17_55

BodEinwohner17_56

BodEinwohner17_57
(WhiB family transcription factor)

BodEinwohner17_58
(helix-turn-helix DNA binding protein)

BodEinwohner17_59
(WhiB family transcription factor)

BodEinwohner17_60
(helix-turn-helix DNA binding protein)

BodEinwohner17_61

BodEinwohner17_62

BodEinwohner17_63

BodEinwohner17_64
(RecA-like DNA recombinase)

BodEinwohner17_65

BodEinwohner17_66
(HNH endonuclease)

BodEinwohner17_67

BodEinwohner17_68

BodEinwohner17_69

BodEinwohner17_70
(DNA helicase/DNA methylase)

BodEinwohner17_71

BodEinwohner17_72

BodEinwohner17_73

BodEinwohner17_74

BodEinwohner17_75
(DNA methylase)

BodEinwohner17_76

BodEinwohner17_77

BodEinwohner17_78

BodEinwohner17_79
(helix-turn-helix DNA binding domain protein)

BodEinwohner17_80

BodEinwohner17_81

BodEinwohner17_82

BodEinwohner17_83

BodEinwohner17_84

BodEinwohner17_85

BodEinwohner17_86

BodEinwohner17_87

BodEinwohner17_88

BodEinwohner17_89

BodEinwohner17_90

BodEinwohner17_91

BodEinwohner17_92

BodEinwohner17_93

BodEinwohner17_94

BodEinwohner17_95

BodEinwohner17_96

BodEinwohner17_97

BodEinwohner17_98

BodEinwohner17_99

BodEinwohner17_100

BodEinwohner17_101

BodEinwohner17_102

BodEinwohner17_103

BodEinwohner17_104
(MerR-like helix-turn-helix DNA binding domain protein)

BodEinwohner17_105
(ribbon-helix-helix DNA binding domain protein)

BodEinwohner17_106

BodEinwohner17_107

BodEinwohner17_108

BodEinwohner17_109

BodEinwohner17_110

BodEinwohner17_111
(glycosyltransferase)

BodEinwohner17_112

BodEinwohner17_113

BodEinwohner17_114

BodEinwohner17_115

BodEinwohner17_116
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMN945900
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
Available Files
Final DNAMaster FileDownload
GenBank File for PhameratorDownload