Detailed Information for Phage NormanBulbieJr |
Discovery Information |
Isolation Host | Mycobacterium smegmatis mc²155 |
Found By | Gillian Black and Vincent Zecchino |
Year Found | 2017 |
Location Found | Philadelphia, PA USA |
Finding Institution | University of the Sciences (now St. Joseph's Univ) |
Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
From enriched soil sample? | No |
Isolation Temperature | Not entered |
GPS Coordinates | 39.9469 N, 75.2071 W Map |
Discovery Notes | We discovered our phage in the soil outside of our university. The phage was dug up into a plastic bag and promptly isolated using direct isolation. |
Naming Notes | This name was chosen to honor one of our lab teaching assistants and for our favorite tool in the phage lab, a bulbie. |
Sequencing Information |
Sequencing Complete? | Yes |
Date Sequencing Completed | Jan 8, 2018 |
Sequencing Facility | Pittsburgh Bacteriophage Institute |
Shotgun Sequencing Method | Illumina Sequencing |
Approximate Shotgun Coverage | 1593 |
Genome length (bp) | 58102 |
Character of genome ends | 3' Sticky Overhang |
Overhang Length | 10 bases |
Overhang Sequence | CGGACGGCGC |
GC Content | 61.3% |
Fasta file available? | Yes: Download fasta file |
Characterization |
Cluster | F |
Subcluster | F1 |
Cluster Life Cycle | Temperate |
Other Cluster Members |
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Annotating Institution | University of the Sciences (now St. Joseph's Univ) |
Annotation Status | In GenBank |
Plaque Notes | The morphology of our phage is lytic. This means that it makes very clear plaques. Our phage tends to form plaques of multiple sizes. |
Morphotype | Siphoviridae |
Has been Phamerated? | Yes |
Gene List |
|
Submitted Annotation in DNA Master Format | Download |
Submitted Minimal DNA Master File | Download |
Author List | Download |
Annotation Cover Sheet | Download |
Publication Info |
Uploaded to GenBank? | Yes |
GenBank Accession | MH399784 |
Refseq Number | None yet |
Archiving Info |
Archiving status |
Archived |
Pitt Freezer Box# |
47 |
Pitt Freezer Box Grid# |
D7 |
Available Files |
GenBank File for Phamerator | Download |