Mycobacterium phage LastJedi
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Detailed Information for Phage LastJedi
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Duplicates of this PhageMargotMini
Found ByJewel Joseph and Don Kiriella
Year Found2018
Location FoundOld Westbury, NY USA
Finding InstitutionSUNY Old Westbury
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature75°C
GPS Coordinates40.798447 N, 73.57308 W Map
Discovery NotesShrubby Area in-between Natural Science Building and Campus Center at SUNY Old Westbury. It had been raining for a few days before and the ground was muddy. It was near a bush.
Naming NotesWe named our phage LastJedi because we are Star Wars fans.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 31, 2019
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1652
Genome length (bp)55149
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGACGGCGC
GC Content61.8%
Fasta file available?Yes: Download fasta file
Characterization
ClusterF
SubclusterF1
Cluster Life CycleTemperate
Other Cluster Members

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Soul22 (None)

Akhila (F1)

Alexphander (F1)

ArcusAngelus (F1)

Ardmore (F1)

Awesomesauce (F1)

Batiatus (F1)

BigPhil (F1)

Bipolar (F1)

Blexus (F1)

BobaPhett (F1)

Bobi (F1)

BodEinwohner17 (F1)

Boomer (F1)

Brocalys (F1)

Bubbles123 (F1)

Burwell21 (F1)

BuzzLyseyear (F1)

ByChance (F1)

Byougenkin (F1)

Cabrinians (F1)

CaptainTrips (F1)

Cerasum (F1)

Che8 (F1)

Chuckly (F1)

Clifton (F1)

Coco12 (F1)

Cornucopia (F1)

Daenerys (F1)

Dante (F1)

DaWorst (F1)

DeadP (F1)

DillTech15 (F1)

DirtMcgirt (F1)

DLane (F1)

Donkeykong (F1)

Doomphist (F1)

Dorothy (F1)

DotProduct (F1)

Doug (F1)

Drago (F1)

DRBy19 (F1)

Eish (F1)

EleanorGeorge (F1)

Emma (F1)

Empress (F1)

Enby (F1)

Estave1 (F1)

Fancypants (F1)

Filuzino (F1)

Firehouse51 (F1)

Flathead (F1)

Florinda (F1)

Frankie (F1)

Fruitloop (F1)

Galactic (F1)

Gandalph (F1)

Geralt (F1)

Girafales (F1)

Girr (F1)

Gorge (F1)

GUmbie (F1)

Hades (F1)

Hamulus (F1)

Harley (F1)

Hegedechwinu (F1)

Hlubikazi (F1)

Ibhubesi (F1)

Inventum (F1)

IrishSherpFalk (F1)

James (F1)

Job42 (F1)

JoeyJr (F1)

Juice456 (F1)

Kenuha5 (F1)

Kersh (F1)

Kimberlium (F1)

KingMidas (F1)

Kingsley (F1)

Koella (F1)

Krakatau (F1)

KristaRAM (F1)

LastJedi (F1)

Latretium (F1)

LilMoolah (F1)

Lizziana (F1)

Llama (F1)

Llij (F1)

Lorde (F1)

Mahavrat (F1)

Mandlovu (F1)

Mantra (F1)

Martik (F1)

Mattes (F1)

Melissauren88 (F1)

MilanaBonita (F1)

MilleniumForce (F1)

MinionDave (F1)

Minnie (F1)

Misha28 (F1)

MisterCuddles (F1)

Modragons (F1)

Moonbeam (F1)

Mova (F1)

Mozy (F1)

Mutaforma13 (F1)

Nimbo (F1)

Nitzel (F1)

Nivrat (F1)

NormanBulbieJr (F1)

Ochi17 (F1)

OfUltron (F1)

Ogopogo (F1)

OldBen (F1)

OlympiaSaint (F1)

Ovechkin (F1)

OwlsT2W (F1)

Pacc40 (F1)

Phanphagia (F1)

PHappiness (F1)

Phasih (F1)

Phatniss (F1)

Piper2020 (F1)

Pippy (F1)

Plumbus (F1)

PMC (F1)

Poenanya (F1)

Polka14 (F1)

Pollywog (F1)

PopTart (F1)

Priscilla (F1)

QuickMath (F1)

Quico (F1)

Ramsey (F1)

RitaG (F1)

RockyHorror (F1)

Royals2015 (F1)

Ruby (F1)

Saal (F1)

Sandalphon (F1)

Sassafras (F1)

SassyB (F1)

Scottish (F1)

Seabastian (F1)

Seagreen (F1)

SG4 (F1)

Shauna1 (F1)

ShiLan (F1)

SimranZ1 (F1)

SiSi (F1)

Sparkdehlily (F1)

Spartacus (F1)

Spikelee (F1)

Spoonbill (F1)

StAnnes (F1)

Strokeseat (F1)

SuperGrey (F1)

SwagPigglett (F1)

Taj (F1)

TDanisky (F1)

TootsiePop (F1)

Tweety (F1)

UncleRicky (F1)

Velveteen (F1)

Veteran (F1)

Wachhund (F1)

Wee (F1)

Whouxphf (F1)

WillSterrel (F1)

XFactor (F1)

Zerg (F1)

Avani (F2)

Che9d (F2)

Demsculpinboyz (F2)

Jabbawokkie (F2)

Yoshi (F2)

Zapner (F2)

Squirty (F3)

Renaud18 (F4)

TChen (F4)

ThetaBob (F4)

Cornie (F5)

Annotating InstitutionCollege of Charleston
Annotation StatusIn GenBank
Plaque NotesThe plaques is close to circular but not perfect, clear plaques with a titer of 5.2 * 10^10 PFU/ml, with a DNA concentration 90 nanograms per microliter.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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LastJedi_1
(terminase small subunit)

LastJedi_2
(terminase large subunit)

LastJedi_3
(portal protein)

LastJedi_4
(capsid maturation protease)

LastJedi_5
(scaffolding protein)

LastJedi_6
(major capsid protein)

LastJedi_7
(head-to-tail adaptor)

LastJedi_8
(head-to-tail stopper)

LastJedi_9

LastJedi_10
(tail terminator)

LastJedi_11
(major tail protein)

LastJedi_12
(tail assembly chaperone)

LastJedi_13
(tail assembly chaperone)

LastJedi_14
(tape measure protein)

LastJedi_15
(minor tail protein)

LastJedi_16
(minor tail protein)

LastJedi_17

LastJedi_18
(minor tail protein)

LastJedi_19

LastJedi_20

LastJedi_21

LastJedi_22

LastJedi_23

LastJedi_24
(lysin A)

LastJedi_25
(lysin B)

LastJedi_26

LastJedi_27

LastJedi_28

LastJedi_29
(DNA polymerase III subunit)

LastJedi_30

LastJedi_31

LastJedi_32

LastJedi_33

LastJedi_34
(tyrosine integrase)

LastJedi_35
(RelE-like toxin)

LastJedi_36
(RelB-like antitoxin)

LastJedi_37

LastJedi_38
(peptidase)

LastJedi_39
(immunity repressor)

LastJedi_40
(Cro protein)

LastJedi_41
(antirepressor)

LastJedi_42

LastJedi_43

LastJedi_44

LastJedi_45

LastJedi_46
(excise)

LastJedi_47

LastJedi_48

LastJedi_49

LastJedi_50

LastJedi_51
(WhiB family transcription factor)

LastJedi_52
(helix-turn-helix DNA binding domain protein)

LastJedi_53
(WhiB family transcription factor)

LastJedi_54
(helix-turn-helix DNA binding domain protein)

LastJedi_55

LastJedi_56

LastJedi_57
(Ku-like dsDNA break-binding protein)

LastJedi_58
(AAA-ATPase)

LastJedi_59
(HNH endonuclease)

LastJedi_60
(DNA helicase)

LastJedi_61

LastJedi_62

LastJedi_63

LastJedi_64

LastJedi_65
(G-I-Y Y-I-G endonuclease)

LastJedi_66

LastJedi_67

LastJedi_68

LastJedi_69

LastJedi_70

LastJedi_71

LastJedi_72

LastJedi_73
(MPME1 protein)

LastJedi_74

LastJedi_75

LastJedi_76

LastJedi_77

LastJedi_78

LastJedi_79

LastJedi_80

LastJedi_81

LastJedi_82
(G-I-Y Y-I-G endonuclease)

LastJedi_83

LastJedi_84

LastJedi_85

LastJedi_86

LastJedi_87

LastJedi_88

LastJedi_89

LastJedi_90

LastJedi_91

LastJedi_92
(glycosyltransferase)

LastJedi_93

LastJedi_94
(glycosyltransferase)

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMT553345
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 88
Pitt Freezer Box Grid# H5
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload