Detailed Information for Phage LastJedi |
Discovery Information |
Isolation Host | Mycobacterium smegmatis mc²155 |
Duplicates of this Phage | MargotMini |
Found By | Jewel Joseph and Don Kiriella |
Year Found | 2018 |
Location Found | Old Westbury, NY USA |
Finding Institution | SUNY Old Westbury |
Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
From enriched soil sample? | Yes |
Isolation Temperature | 75°C |
GPS Coordinates | 40.798447 N, 73.57308 W Map |
Discovery Notes | Shrubby Area in-between Natural Science Building and Campus Center at SUNY Old Westbury. It had been raining for a few days before and the ground was muddy. It was near a bush. |
Naming Notes | We named our phage LastJedi because we are Star Wars fans. |
Sequencing Information |
Sequencing Complete? | Yes |
Date Sequencing Completed | Jan 31, 2019 |
Sequencing Facility | Pittsburgh Bacteriophage Institute |
Shotgun Sequencing Method | Illumina Sequencing |
Approximate Shotgun Coverage | 1652 |
Genome length (bp) | 55149 |
Character of genome ends | 3' Sticky Overhang |
Overhang Length | 10 bases |
Overhang Sequence | CGGACGGCGC |
GC Content | 61.8% |
Fasta file available? | Yes: Download fasta file |
Characterization |
Cluster | F |
Subcluster | F1 |
Cluster Life Cycle | Temperate |
Other Cluster Members |
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Annotating Institution | College of Charleston |
Annotation Status | In GenBank |
Plaque Notes | The plaques is close to circular but not perfect, clear plaques with a titer of 5.2 * 10^10 PFU/ml, with a DNA concentration 90 nanograms per microliter. |
Morphotype | Siphoviridae |
Has been Phamerated? | Yes |
Gene List |
|
Submitted Annotation in DNA Master Format | Download |
Submitted Minimal DNA Master File | Download |
Author List | Download |
Annotation Cover Sheet | Download |
Publication Info |
Uploaded to GenBank? | Yes |
GenBank Accession | MT553345 |
Refseq Number | None yet |
Published in Paper | Davis et al 2020, Microbiology Resource Announcements: Genome Sequences of the Mycobacteriophages Awesomesauce and LastJedi |
Archiving Info |
Archiving status |
Archived |
Pitt Freezer Box# |
88 |
Pitt Freezer Box Grid# |
H5 |
Available Files |
Plaque Picture | Download |
Restriction Digest Picture | Download |
EM Picture | Download |
GenBank File for Phamerator | Download |