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Mycobacterium phage Mahavrat
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Detailed Information for Phage Mahavrat
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByDeeya Patel
Year Found2016
Location FoundHopkinsville, KY USA
Finding InstitutionWestern Kentucky University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature72°C
GPS Coordinates36.9855 N, 86.4492 W Map
Discovery NotesPhage Mahavrat was isolated on 9/27/16 from moist dark brown soil rich with decaying plant matter, at a depth of 12 cm. The soil was taken near a greenhouse next to the Engineering and Biological Sciences Building at Western Kentucky University. The temperature was 76F at time of collection. Mahavrat was isolated using an enrichment culture incubated at 30C.
Naming NotesThis phage is named after my grandfather, Mahavrat, the biggest influencer on my education.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJul 3, 2017
Sequencing FacilityNC State Genomic Sciences Laboratory
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage268
Genome length (bp)55945
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGAAGGCGC
GC Content61.3%
Fasta file available?Yes: Download fasta file
Characterization
ClusterF
SubclusterF1
Cluster Life CycleTemperate
Other Cluster Members

Click to View

Renaud18 (None)

Alexphander (F1)

ArcusAngelus (F1)

Ardmore (F1)

Awesomesauce (F1)

Batiatus (F1)

BigPhil (F1)

Bipolar (F1)

Blexus (F1)

BobaPhett (F1)

Bobi (F1)

BodEinwohner17 (F1)

Boomer (F1)

Brocalys (F1)

Bubbles123 (F1)

Burwell21 (F1)

BuzzLyseyear (F1)

ByChance (F1)

Byougenkin (F1)

Cabrinians (F1)

CaptainTrips (F1)

Cerasum (F1)

Che8 (F1)

Chuckly (F1)

Clifton (F1)

Cornucopia (F1)

Daenerys (F1)

Dante (F1)

DaWorst (F1)

DeadP (F1)

DillTech15 (F1)

DirtMcgirt (F1)

DLane (F1)

Donkeykong (F1)

Doomphist (F1)

Dorothy (F1)

DotProduct (F1)

Doug (F1)

Drago (F1)

DRBy19 (F1)

Eish (F1)

EleanorGeorge (F1)

Emma (F1)

Empress (F1)

Enby (F1)

Estave1 (F1)

Fancypants (F1)

Filuzino (F1)

Firehouse51 (F1)

Flathead (F1)

Florinda (F1)

Frankie (F1)

Fruitloop (F1)

Galactic (F1)

Geralt (F1)

Girafales (F1)

Girr (F1)

Gorge (F1)

GUmbie (F1)

Hades (F1)

Hamulus (F1)

Harley (F1)

Hegedechwinu (F1)

Ibhubesi (F1)

Inventum (F1)

IrishSherpFalk (F1)

James (F1)

Job42 (F1)

JoeyJr (F1)

Juice456 (F1)

Kenuha5 (F1)

Kersh (F1)

Kimberlium (F1)

KingMidas (F1)

Kingsley (F1)

Koella (F1)

Krakatau (F1)

KristaRAM (F1)

LastJedi (F1)

Latretium (F1)

LilMoolah (F1)

Lizziana (F1)

Llama (F1)

Llij (F1)

Lorde (F1)

Mahavrat (F1)

Mantra (F1)

Mattes (F1)

Melissauren88 (F1)

MilleniumForce (F1)

MinionDave (F1)

Minnie (F1)

Misha28 (F1)

MisterCuddles (F1)

Modragons (F1)

Mozy (F1)

Mutaforma13 (F1)

Nimbo (F1)

Nitzel (F1)

Nivrat (F1)

NormanBulbieJr (F1)

Ochi17 (F1)

OfUltron (F1)

Ogopogo (F1)

OldBen (F1)

OlympiaSaint (F1)

Ovechkin (F1)

OwlsT2W (F1)

Pacc40 (F1)

PHappiness (F1)

Phasih (F1)

Phatniss (F1)

Piper2020 (F1)

Pippy (F1)

Plumbus (F1)

PMC (F1)

Poenanya (F1)

Polka14 (F1)

Pollywog (F1)

PopTart (F1)

Priscilla (F1)

QuickMath (F1)

Quico (F1)

Ramsey (F1)

RitaG (F1)

RockyHorror (F1)

Royals2015 (F1)

Ruby (F1)

Saal (F1)

Sandalphon (F1)

SassyB (F1)

Scottish (F1)

Seagreen (F1)

SG4 (F1)

Shauna1 (F1)

ShiLan (F1)

SimranZ1 (F1)

SiSi (F1)

Sparkdehlily (F1)

Spartacus (F1)

Spikelee (F1)

Spoonbill (F1)

StAnnes (F1)

Strokeseat (F1)

SuperGrey (F1)

SwagPigglett (F1)

Taj (F1)

TDanisky (F1)

TootsiePop (F1)

Tweety (F1)

UncleRicky (F1)

Velveteen (F1)

Wachhund (F1)

Wee (F1)

Whouxphf (F1)

WillSterrel (F1)

XFactor (F1)

Zerg (F1)

Avani (F2)

Che9d (F2)

Demsculpinboyz (F2)

Jabbawokkie (F2)

Yoshi (F2)

Zapner (F2)

Squirty (F3)

TChen (F4)

ThetaBob (F4)

Cornie (F5)

Annotation StatusIn GenBank
Plaque NotesPlaques were clear, lytic and circular in their morphology.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

Click to View

Mahavrat_1
(terminase, small subunit)

Mahavrat_2
(terminase, large subunit)

Mahavrat_3
(portal protein)

Mahavrat_4
(capsid maturation protease)

Mahavrat_5
(scaffolding protein)

Mahavrat_6
(major capsid protein)

Mahavrat_7
(head-to-tail adaptor)

Mahavrat_8
(head-to-tail stopper)

Mahavrat_9

Mahavrat_10
(tail terminator)

Mahavrat_11
(major tail protein)

Mahavrat_12
(tail assembly chaperone)

Mahavrat_13
(tail assembly chaperone)

Mahavrat_14
(tape measure protein)

Mahavrat_15
(minor tail protein)

Mahavrat_16
(minor tail protein)

Mahavrat_17
(minor tail protein)

Mahavrat_18
(minor tail protein)

Mahavrat_19
(minor tail protein, D-ala-D-ala carboxypeptidase)

Mahavrat_20

Mahavrat_21

Mahavrat_22

Mahavrat_23
(minor tail protein)

Mahavrat_24

Mahavrat_25
(helix-turn-helix DNA binding domain)

Mahavrat_26

Mahavrat_27

Mahavrat_28
(membrane protein)

Mahavrat_29

Mahavrat_30
(lysin A)

Mahavrat_31
(lysin B)

Mahavrat_32
(holin)

Mahavrat_33
(membrane protein)

Mahavrat_34

Mahavrat_35
(exonuclease)

Mahavrat_36

Mahavrat_37
(membrane protein)

Mahavrat_38

Mahavrat_39
(tyrosine integrase)

Mahavrat_40
(membrane protein)

Mahavrat_41

Mahavrat_42
(immunity repressor)

Mahavrat_43
(Cro (control of repressor`s operator))

Mahavrat_44
(antirepressor)

Mahavrat_45

Mahavrat_46

Mahavrat_47

Mahavrat_48
(excise)

Mahavrat_49

Mahavrat_50

Mahavrat_51

Mahavrat_52

Mahavrat_53

Mahavrat_54

Mahavrat_55
(WhiB family transcription factor)

Mahavrat_56

Mahavrat_57
(WhiB family transcription factor)

Mahavrat_58
(helix-turn-helix DNA binding domain)

Mahavrat_59

Mahavrat_60

Mahavrat_61

Mahavrat_62
(HNH endonuclease)

Mahavrat_63
(DNA methylase)

Mahavrat_64

Mahavrat_65

Mahavrat_66

Mahavrat_67

Mahavrat_68

Mahavrat_69

Mahavrat_70

Mahavrat_71

Mahavrat_72

Mahavrat_73

Mahavrat_74

Mahavrat_75

Mahavrat_76

Mahavrat_77

Mahavrat_78

Mahavrat_79

Mahavrat_80

Mahavrat_81

Mahavrat_82

Mahavrat_83

Mahavrat_84

Mahavrat_85

Mahavrat_86

Mahavrat_87

Mahavrat_88

Mahavrat_89

Mahavrat_90
(helix-turn-helix DNA binding domain)

Mahavrat_91

Mahavrat_92

Mahavrat_93

Mahavrat_94
(glycosyltransferase)

Mahavrat_95

Mahavrat_96
(serine/threonine kinase)

Mahavrat_97
(glycosyltransferase)

Mahavrat_98
(HNH endonuclease)

Mahavrat_99
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK967397
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Designator 2016WEKUmahavratDP
SEA Lysate Titer 3.8 x 10^12 pfu/mL
Date of SEA Lysate Titering Nov 15, 2016
Pitt Freezer Box# 37
Pitt Freezer Box Grid# C10
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload