Mycobacterium phage Piper2020
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Detailed Information for Phage Piper2020
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByDanielle Galli and Micaela Caron
Year Found2016
Location FoundMelrose, MA USA
Finding InstitutionMerrimack College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation TemperatureNot entered
GPS Coordinates42.451333 N, 71.076333 W Map
Discovery NotesThe dirt sample that was tested was adopted from our Professor. It came from a tomato plant that was watered daily throughout the summer.
Naming NotesOur phage that we made in class out of different materials was named Piper. Micaela and I thought that this was an adorable name so we decided to name our discovered phage Piper.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 10, 2017
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1883
Genome length (bp)57703
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCCGAAGGCAT
GC Content62.7%
Fasta file available?Yes: Download fasta file
Characterization
ClusterF
SubclusterF1
Cluster Life CycleTemperate
Other Cluster Members

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Alexphander (F1)

ArcusAngelus (F1)

Ardmore (F1)

Awesomesauce (F1)

Batiatus (F1)

BigPhil (F1)

Bipolar (F1)

Blexus (F1)

BobaPhett (F1)

Bobi (F1)

BodEinwohner17 (F1)

Boomer (F1)

Brocalys (F1)

Bubbles123 (F1)

Burwell21 (F1)

BuzzLyseyear (F1)

ByChance (F1)

Byougenkin (F1)

Cabrinians (F1)

CaptainTrips (F1)

Cerasum (F1)

Che8 (F1)

Chuckly (F1)

Clifton (F1)

Coco12 (F1)

Cornucopia (F1)

Daenerys (F1)

Dante (F1)

DaWorst (F1)

DeadP (F1)

DillTech15 (F1)

DirtMcgirt (F1)

DLane (F1)

Donkeykong (F1)

Doomphist (F1)

Dorothy (F1)

DotProduct (F1)

Doug (F1)

Drago (F1)

DRBy19 (F1)

Eish (F1)

EleanorGeorge (F1)

Emma (F1)

Empress (F1)

Enby (F1)

Estave1 (F1)

Fancypants (F1)

Filuzino (F1)

Firehouse51 (F1)

Flathead (F1)

Florinda (F1)

Frankie (F1)

Fruitloop (F1)

Galactic (F1)

Gandalph (F1)

Geralt (F1)

Girafales (F1)

Girr (F1)

Gorge (F1)

GUmbie (F1)

Hades (F1)

Hamulus (F1)

Harley (F1)

Hegedechwinu (F1)

Ibhubesi (F1)

Inventum (F1)

IrishSherpFalk (F1)

James (F1)

Job42 (F1)

JoeyJr (F1)

Juice456 (F1)

Kenuha5 (F1)

Kersh (F1)

Kimberlium (F1)

KingMidas (F1)

Kingsley (F1)

Koella (F1)

Krakatau (F1)

KristaRAM (F1)

LastJedi (F1)

Latretium (F1)

LilMoolah (F1)

Lizziana (F1)

Llama (F1)

Llij (F1)

Lorde (F1)

Mahavrat (F1)

Mantra (F1)

Mattes (F1)

Melissauren88 (F1)

MilleniumForce (F1)

MinionDave (F1)

Minnie (F1)

Misha28 (F1)

MisterCuddles (F1)

Modragons (F1)

Mozy (F1)

Mutaforma13 (F1)

Nimbo (F1)

Nitzel (F1)

Nivrat (F1)

NormanBulbieJr (F1)

Ochi17 (F1)

OfUltron (F1)

Ogopogo (F1)

OldBen (F1)

OlympiaSaint (F1)

Ovechkin (F1)

OwlsT2W (F1)

Pacc40 (F1)

Phanphagia (F1)

PHappiness (F1)

Phasih (F1)

Phatniss (F1)

Piper2020 (F1)

Pippy (F1)

Plumbus (F1)

PMC (F1)

Poenanya (F1)

Polka14 (F1)

Pollywog (F1)

PopTart (F1)

Priscilla (F1)

QuickMath (F1)

Quico (F1)

Ramsey (F1)

RitaG (F1)

RockyHorror (F1)

Royals2015 (F1)

Ruby (F1)

Saal (F1)

Sandalphon (F1)

SassyB (F1)

Scottish (F1)

Seabastian (F1)

Seagreen (F1)

SG4 (F1)

Shauna1 (F1)

ShiLan (F1)

SimranZ1 (F1)

SiSi (F1)

Sparkdehlily (F1)

Spartacus (F1)

Spikelee (F1)

Spoonbill (F1)

StAnnes (F1)

Strokeseat (F1)

SuperGrey (F1)

SwagPigglett (F1)

Taj (F1)

TDanisky (F1)

TootsiePop (F1)

Tweety (F1)

UncleRicky (F1)

Velveteen (F1)

Veteran (F1)

Wachhund (F1)

Wee (F1)

Whouxphf (F1)

WillSterrel (F1)

XFactor (F1)

Zerg (F1)

Avani (F2)

Che9d (F2)

Demsculpinboyz (F2)

Jabbawokkie (F2)

Yoshi (F2)

Zapner (F2)

Squirty (F3)

Renaud18 (F4)

TChen (F4)

ThetaBob (F4)

Cornie (F5)

Annotating InstitutionMerrimack College
Annotation StatusIn GenBank
Plaque NotesThe plaques that we obtained during this experiment were small and clear.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Piper2020_1
(HNH endonuclease)

Piper2020_2
(terminase small subunit)

Piper2020_3
(terminase large subunit)

Piper2020_4
(portal protein)

Piper2020_5

Piper2020_6
(scaffolding protein)

Piper2020_7
(major capsid protein)

Piper2020_8
(head-to-tail adaptor)

Piper2020_9
(head-to-tail stopper)

Piper2020_10

Piper2020_11
(tail terminator)

Piper2020_12
(major tail protein)

Piper2020_13
(tail assembly chaperone)

Piper2020_14
(tail assembly chaperone)

Piper2020_15
(tapemeasure protein)

Piper2020_16
(minor tail protein)

Piper2020_17
(minor tail protein)

Piper2020_18
(minor tail protein)

Piper2020_19
(minor tail protein)

Piper2020_20
(minor tail protein)

Piper2020_21
(minor tail protein)

Piper2020_22
(minor tail protein)

Piper2020_23
(HNH endonuclease)

Piper2020_24

Piper2020_25

Piper2020_26

Piper2020_27
(helix-turn-helix DNA-binding domain protein)

Piper2020_28

Piper2020_29

Piper2020_30

Piper2020_31

Piper2020_32
(lysin A)

Piper2020_33
(lysin B)

Piper2020_34

Piper2020_35

Piper2020_36

Piper2020_37
(DnaQ-like exonuclease)

Piper2020_38

Piper2020_39

Piper2020_40

Piper2020_41
(integrase)

Piper2020_42

Piper2020_43

Piper2020_44
(immunity repressor)

Piper2020_45

Piper2020_46

Piper2020_47

Piper2020_48

Piper2020_49

Piper2020_50

Piper2020_51

Piper2020_52

Piper2020_53

Piper2020_54

Piper2020_55

Piper2020_56

Piper2020_57

Piper2020_58

Piper2020_59

Piper2020_60
(WhiB family transcription factor)

Piper2020_61
(membrane protein)

Piper2020_62

Piper2020_63
(MPME 1 protein)

Piper2020_64

Piper2020_65

Piper2020_66

Piper2020_67
(Cas4 family exonuclease)

Piper2020_68

Piper2020_69

Piper2020_70

Piper2020_71
(RusA-like resolvase)

Piper2020_72

Piper2020_73

Piper2020_74
(helix-turn-helix DNA-binding domain protein)

Piper2020_75
(DNA methylase)

Piper2020_76

Piper2020_77
(DNA methylase)

Piper2020_78

Piper2020_79

Piper2020_80

Piper2020_81

Piper2020_82

Piper2020_83

Piper2020_84

Piper2020_85
(HNH endonuclease)

Piper2020_86

Piper2020_87

Piper2020_88

Piper2020_89

Piper2020_90

Piper2020_91
(helix-turn-helix DNA-binding domain protein)

Piper2020_92

Piper2020_93

Piper2020_94

Piper2020_95

Piper2020_96
(glycosyltransferase)

Piper2020_97

Piper2020_98
(glycosyltransferase)

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK494120
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 6.3*10^10
Pitt Freezer Box# 73
Pitt Freezer Box Grid# H2
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload