Mycobacterium phage Fastidio
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Detailed Information for Phage Fastidio
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByHyunbi H, Ethan W, Payson D, Matt J
Year Found2023
Location FoundProvo, UT United States
Finding InstitutionBrigham Young University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature29°C
GPS Coordinates40.214582 N, 111.618295 W Map
Discovery NotesCollected from soil in Utah County.
Naming NotesI had to do a process called 10 plate a couple times in order to create a sufficiently large high-titer lysate. This annoyed me a little bit because I didn't understand why it wasn't working. Therefore I named it "fastidio" which is annoyance in spanish.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedApr 24, 2024
Sequencing FacilityCD Genomics
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage3593
Genome length (bp)55839
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGACGGCGC
GC Content61.6%
Fasta file available?Yes: Download fasta file
Characterization
ClusterF
SubclusterF1
Cluster Life CycleTemperate
Other Cluster Members

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Blarby (None)

MulchSalad (None)

Akhila (F1)

Alexphander (F1)

AlpineSix (F1)

ArcusAngelus (F1)

Ardmore (F1)

Aubs (F1)

Awesomesauce (F1)

Batiatus (F1)

Beakin (F1)

BigPhil (F1)

Bipolar (F1)

Blexus (F1)

BlueCrab (F1)

BobaPhett (F1)

Bobi (F1)

BodEinwohner17 (F1)

Boomer (F1)

Brocalys (F1)

Bubbles123 (F1)

Burwell21 (F1)

BuzzLyseyear (F1)

ByChance (F1)

Byougenkin (F1)

Cabrinians (F1)

CaptainTrips (F1)

Cerasum (F1)

Che8 (F1)

Chevrolet (F1)

ChickenDinner (F1)

Chuckly (F1)

Clifton (F1)

Coco12 (F1)

Cornucopia (F1)

Crumble (F1)

DaddyRickover (F1)

Daenerys (F1)

Dante (F1)

DaWorst (F1)

DeadP (F1)

Deb65 (F1)

DillTech15 (F1)

DirtMcgirt (F1)

DLane (F1)

DocMcStuffins (F1)

Donkeykong (F1)

Doomphist (F1)

Dorothy (F1)

DosHalletts (F1)

DotProduct (F1)

Doug (F1)

Drago (F1)

DRBy19 (F1)

Eish (F1)

EleanorGeorge (F1)

Emma (F1)

Empress (F1)

Enby (F1)

Eradicator (F1)

Estave1 (F1)

Fancypants (F1)

Fastidio (F1)

Filuzino (F1)

Firehouse51 (F1)

Flathead (F1)

Florinda (F1)

Frankie (F1)

FreddyB (F1)

Fruitloop (F1)

Galactic (F1)

Gandalph (F1)

Geralt (F1)

GigiOuiOui (F1)

Girafales (F1)

Girr (F1)

Gorge (F1)

GUmbie (F1)

Guppsters (F1)

Hades (F1)

Hamulus (F1)

Harley (F1)

Hegedechwinu (F1)

Hlubikazi (F1)

Ibhubesi (F1)

IbOuu (F1)

IMpilo (F1)

Inventum (F1)

IrishSherpFalk (F1)

JalFarm20 (F1)

James (F1)

Jarcob (F1)

Jinglebell (F1)

Job42 (F1)

JoeyJr (F1)

Juice456 (F1)

Juniper1 (F1)

Karhdo (F1)

Kenuha5 (F1)

Kersh (F1)

Kimberlium (F1)

KingMidas (F1)

Kingsley (F1)

Koella (F1)

Krakatau (F1)

KristaRAM (F1)

LastJedi (F1)

Latretium (F1)

Leozinho (F1)

Leperchaun (F1)

LilMoolah (F1)

LittleShirley (F1)

Lizziana (F1)

Llama (F1)

Llij (F1)

Lorde (F1)

LunaBlu (F1)

Madiba (F1)

MadMen (F1)

Mahavrat (F1)

Mandlovu (F1)

Mantra (F1)

Maravista (F1)

Marker (F1)

Martik (F1)

Mattes (F1)

Melissauren88 (F1)

MilanaBonita (F1)

MilleniumForce (F1)

MinionDave (F1)

Minnie (F1)

Misha28 (F1)

MisterCuddles (F1)

Modragons (F1)

Moonbeam (F1)

Mova (F1)

Mozy (F1)

Ms6 (F1)

MulchExplorer (F1)

Mutaforma13 (F1)

Nimbo (F1)

Nitzel (F1)

Nivrat (F1)

NormanBulbieJr (F1)

Ochi17 (F1)

OfUltron (F1)

Ogopogo (F1)

Oksu (F1)

OldBen (F1)

OlympiaSaint (F1)

Ovechkin (F1)

OwlsT2W (F1)

Pacc40 (F1)

Phalconet (F1)

Phanphagia (F1)

PHappiness (F1)

Phasih (F1)

Phatniss (F1)

PhesterPhotato (F1)

Philus (F1)

Piper2020 (F1)

Pippy (F1)

Plumbus (F1)

PMC (F1)

Poenanya (F1)

Polka14 (F1)

Pollywog (F1)

PopTart (F1)

Priscilla (F1)

QuickMath (F1)

Quico (F1)

Radiance (F1)

Ramsey (F1)

RedBird (F1)

Rialto (F1)

Rita (F1)

RitaG (F1)

Rockne (F1)

RockyHorror (F1)

Royals2015 (F1)

Ruby (F1)

Saal (F1)

Sabbb (F1)

Sandalphon (F1)

Sarma624 (F1)

Sassafras (F1)

SassyB (F1)

Scottish (F1)

Seabastian (F1)

Seagreen (F1)

SG4 (F1)

Shauna1 (F1)

ShiLan (F1)

ShowerHandel (F1)

ShroomBoi (F1)

SimranZ1 (F1)

SiSi (F1)

Sparkdehlily (F1)

Spartacus (F1)

Spikelee (F1)

Spoonbill (F1)

StAnnes (F1)

Stap (F1)

Starcevich (F1)

Strokeseat (F1)

SuperGrey (F1)

SwagPigglett (F1)

Taj (F1)

TDanisky (F1)

TootsiePop (F1)

Tootsieroll (F1)

Totinger (F1)

Tweety (F1)

UncleRicky (F1)

Velveteen (F1)

Veteran (F1)

VRedHorse (F1)

Wachhund (F1)

Wee (F1)

Whatsapiecost (F1)

Whouxphf (F1)

WillSterrel (F1)

XFactor (F1)

Yorick (F1)

Zerg (F1)

Zizzle (F1)

Avani (F2)

Che9d (F2)

Demsculpinboyz (F2)

Jabbawokkie (F2)

Soul22 (F2)

Yoshi (F2)

Zapner (F2)

Squirty (F3)

LunaStella (F4)

Renaud18 (F4)

TChen (F4)

ThetaBob (F4)

Cornie (F5)

Toron (F6)

Annotating InstitutionBrigham Young University
Annotation StatusIn GenBank
MorphotypeSiphoviridae
Number of Genes105
Number of tRNAs1
Has been Phamerated?Yes
Gene List

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Fastidio_1
(terminase, small subunit)

Fastidio_2
(terminase, large subunit)

Fastidio_3
(portal protein)

Fastidio_4
(capsid maturation protease)

Fastidio_5
(scaffolding protein)

Fastidio_6
(major capsid protein)

Fastidio_7
(head-to-tail adaptor)

Fastidio_8
(head-to-tail stopper)

Fastidio_9

Fastidio_10
(tail terminator)

Fastidio_11
(major tail protein)

Fastidio_12
(tail assembly chaperone)

Fastidio_13
(tail assembly chaperone)

Fastidio_14
(tape measure protein)

Fastidio_15
(minor tail protein)

Fastidio_16
(minor tail protein)

Fastidio_17

Fastidio_18
(minor tail protein)

Fastidio_19
(minor tail protein, D-ala-D-ala carboxypeptidase)

Fastidio_20
(minor tail protein)

Fastidio_21

Fastidio_22

Fastidio_23

Fastidio_24

Fastidio_25
(helix-turn-helix DNA binding domain)

Fastidio_26

Fastidio_27

Fastidio_28

Fastidio_29

Fastidio_30

Fastidio_31

Fastidio_32
(lysin A)

Fastidio_33
(lysin B)

Fastidio_34
(holin)

Fastidio_35

Fastidio_36

Fastidio_37
(DnaQ-like (DNA polymerase III subunit))

Fastidio_38

Fastidio_39

Fastidio_40

Fastidio_41

Fastidio_42

Fastidio_43

Fastidio_44
(helix-turn-helix DNA binding domain)

Fastidio_45

Fastidio_46
(tyrosine integrase)

Fastidio_47

Fastidio_48
(helix-turn-helix DNA binding domain)

Fastidio_49
(antirepressor)

Fastidio_50

Fastidio_51
(helix-turn-helix DNA binding domain)

Fastidio_52

Fastidio_53

Fastidio_54

Fastidio_55

Fastidio_56

Fastidio_57
(WhiB family transcription factor)

Fastidio_58
(helix-turn-helix DNA binding domain)

Fastidio_59
(Whib family transcription factor)

Fastidio_60
(helix-turn-helix DNA binding domain)

Fastidio_61

Fastidio_62

Fastidio_63

Fastidio_64
(ASCE ATPase)

Fastidio_65

Fastidio_66
(HNH endonuclease)

Fastidio_67
(DNA methyltransferase)

Fastidio_68

Fastidio_69

Fastidio_70

Fastidio_71

Fastidio_72

Fastidio_73

Fastidio_74

Fastidio_75

Fastidio_76

Fastidio_77
(ribbon-helix-helix DNA binding domain)

Fastidio_78

Fastidio_79

Fastidio_80

Fastidio_81

Fastidio_82

Fastidio_83

Fastidio_84

Fastidio_85
(mycobacteriophage mobile element 1 (MPME 1))

Fastidio_86

Fastidio_87

Fastidio_88

Fastidio_89

Fastidio_90

Fastidio_91

Fastidio_92

Fastidio_93

Fastidio_94

Fastidio_95

Fastidio_96

Fastidio_97

Fastidio_98
(helix-turn-helix DNA binding domain)

Fastidio_99

Fastidio_100

Fastidio_101

Fastidio_102

Fastidio_103

Fastidio_104
(glycosyltransferase)

Fastidio_105

Fastidio_106

Fastidio_107
(glycosyltransferase)

Fastidio_108
(HNH endonuclease)

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionPQ244011
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
Available Files
EM PictureDownload
GenBank File for PhameratorDownload