The Actinobacteriophage Database | Phage PhesterPhotato

Mycobacterium phage PhesterPhotato

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Detailed Information for Phage PhesterPhotato
Discovery Information
Isolation Host	Mycobacterium smegmatis mc²155
Found By	Alivia Colgan & Aubrey Neary
Year Found	2023
Location Found	New Oxford, PA United States
Finding Institution	Saint Joseph's University
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	Yes
Isolation Temperature	37°C
GPS Coordinates	39.83976 N, 77.0523 W Map
Discovery Notes	Extremely rural location. No roads nearby and closest trees are approximately 12 meters away. Soil was collected from a garden bed. 80% humidity at time of collection. Sunny weather conditions.
Naming Notes	This soil sample was collected from the discoverer's father's garden. Specifically, the bed of soil that it was collected from previously grew potatoes. As a young child, her father always joked that if she were a boy, she would have been named "Fester." The name chosen combines these two ideas with the addition of "Ph" for phage. Therefore, the name of this phage is Phester Photato.
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Jan 12, 2024
Sequencing Facility	Pittsburgh Bacteriophage Institute
Shotgun Sequencing Method	Illumina Sequencing
Approximate Shotgun Coverage	1551
Genome length (bp)	57622
Character of genome ends	3' Sticky Overhang
Overhang Length	10 bases
Overhang Sequence	CGGACGGCGC
GC Content	61.7%
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	F
Subcluster	F1
Cluster Life Cycle	Temperate
Other Cluster Members	Click to View Blarby (None) MulchSalad (None) Akhila (F1) Alexphander (F1) AlpineSix (F1) ArcusAngelus (F1) Ardmore (F1) Aubs (F1) Awesomesauce (F1) Batiatus (F1) Beakin (F1) BigPhil (F1) Bipolar (F1) Blexus (F1) BlueCrab (F1) BobaPhett (F1) Bobi (F1) BodEinwohner17 (F1) Boomer (F1) Brocalys (F1) Bubbles123 (F1) Burwell21 (F1) BuzzLyseyear (F1) ByChance (F1) Byougenkin (F1) Cabrinians (F1) CaptainTrips (F1) Cerasum (F1) Che8 (F1) Chevrolet (F1) ChickenDinner (F1) Chuckly (F1) Clifton (F1) Coco12 (F1) Cornucopia (F1) Crumble (F1) DaddyRickover (F1) Daenerys (F1) Dante (F1) DaWorst (F1) DeadP (F1) Deb65 (F1) DillTech15 (F1) DirtMcgirt (F1) DLane (F1) DocMcStuffins (F1) Donkeykong (F1) Doomphist (F1) Dorothy (F1) DosHalletts (F1) DotProduct (F1) Doug (F1) Drago (F1) DRBy19 (F1) Eish (F1) EleanorGeorge (F1) Emma (F1) Empress (F1) Enby (F1) Eradicator (F1) Estave1 (F1) Fancypants (F1) Fastidio (F1) Filuzino (F1) Firehouse51 (F1) Flathead (F1) Florinda (F1) Frankie (F1) FreddyB (F1) Fruitloop (F1) Galactic (F1) Gandalph (F1) Geralt (F1) GigiOuiOui (F1) Girafales (F1) Girr (F1) Gorge (F1) GUmbie (F1) Guppsters (F1) Hades (F1) Hamulus (F1) Harley (F1) Hegedechwinu (F1) Hlubikazi (F1) Ibhubesi (F1) IbOuu (F1) IMpilo (F1) Inventum (F1) IrishSherpFalk (F1) JalFarm20 (F1) James (F1) Jarcob (F1) Jinglebell (F1) Job42 (F1) JoeyJr (F1) Juice456 (F1) Juniper1 (F1) Karhdo (F1) Kenuha5 (F1) Kersh (F1) Kimberlium (F1) KingMidas (F1) Kingsley (F1) Koella (F1) Krakatau (F1) KristaRAM (F1) LastJedi (F1) Latretium (F1) Leozinho (F1) Leperchaun (F1) LilMoolah (F1) LittleShirley (F1) Lizziana (F1) Llama (F1) Llij (F1) Lorde (F1) LunaBlu (F1) Madiba (F1) MadMen (F1) Mahavrat (F1) Mandlovu (F1) Mantra (F1) Maravista (F1) Marker (F1) Martik (F1) Mattes (F1) Melissauren88 (F1) MilanaBonita (F1) MilleniumForce (F1) MinionDave (F1) Minnie (F1) Misha28 (F1) MisterCuddles (F1) Modragons (F1) Moonbeam (F1) Mova (F1) Mozy (F1) Ms6 (F1) MulchExplorer (F1) Mutaforma13 (F1) Nimbo (F1) Nitzel (F1) Nivrat (F1) NormanBulbieJr (F1) Ochi17 (F1) OfUltron (F1) Ogopogo (F1) Oksu (F1) OldBen (F1) OlympiaSaint (F1) Ovechkin (F1) OwlsT2W (F1) Pacc40 (F1) Phalconet (F1) Phanphagia (F1) PHappiness (F1) Phasih (F1) Phatniss (F1) PhesterPhotato (F1) Philus (F1) Piper2020 (F1) Pippy (F1) Plumbus (F1) PMC (F1) Poenanya (F1) Polka14 (F1) Pollywog (F1) PopTart (F1) Priscilla (F1) QuickMath (F1) Quico (F1) Radiance (F1) Ramsey (F1) RedBird (F1) Rialto (F1) Rita (F1) RitaG (F1) Rockne (F1) RockyHorror (F1) Royals2015 (F1) Ruby (F1) Saal (F1) Sabbb (F1) Sandalphon (F1) Sarma624 (F1) Sassafras (F1) SassyB (F1) Scottish (F1) Seabastian (F1) Seagreen (F1) SG4 (F1) Shauna1 (F1) ShiLan (F1) ShowerHandel (F1) ShroomBoi (F1) SimranZ1 (F1) SiSi (F1) Sparkdehlily (F1) Spartacus (F1) Spikelee (F1) Spoonbill (F1) StAnnes (F1) Stap (F1) Starcevich (F1) Strokeseat (F1) SuperGrey (F1) SwagPigglett (F1) Taj (F1) TDanisky (F1) TootsiePop (F1) Tootsieroll (F1) Totinger (F1) Tweety (F1) UncleRicky (F1) Velveteen (F1) Veteran (F1) VRedHorse (F1) Wachhund (F1) Wee (F1) Whatsapiecost (F1) Whouxphf (F1) WillSterrel (F1) XFactor (F1) Yorick (F1) Zerg (F1) Zizzle (F1) Avani (F2) Che9d (F2) Demsculpinboyz (F2) Jabbawokkie (F2) Soul22 (F2) Yoshi (F2) Zapner (F2) Squirty (F3) LunaStella (F4) Renaud18 (F4) TChen (F4) ThetaBob (F4) Cornie (F5) Toron (F6)
Annotating Institution	Saint Joseph's University
Annotation Status	In GenBank
Plaque Notes	Most plaques have diameters of 7 mm while a few have diameters of 4 mm. Every plaque has three rings to form a distinct bullseye: a very clear center, a less clear middle ring, and a cloudy outer ring. The plaques are turbid rather than clear.
Morphotype	Siphoviridae
Has been Phamerated?	Yes
Gene List	Click to View PhesterPhotato_1 (terminase small subunit) PhesterPhotato_2 (terminase large subunit) PhesterPhotato_3 (portal protein) PhesterPhotato_4 (capsid maturation protease) PhesterPhotato_5 (scaffolding protein) PhesterPhotato_6 (major capsid protein) PhesterPhotato_7 (head-to-tail adaptor) PhesterPhotato_8 (head-to-tail stopper) PhesterPhotato_9 PhesterPhotato_10 (tail terminator) PhesterPhotato_11 (major tail protein) PhesterPhotato_12 (tail assembly chaperone) PhesterPhotato_13 (tail assembly chaperone) PhesterPhotato_14 (tape measure protein) PhesterPhotato_15 (minor tail protein) PhesterPhotato_16 (minor tail protein) PhesterPhotato_17 (minor tail protein) PhesterPhotato_18 (minor tail protein) PhesterPhotato_19 (minor tail protein D-ala-D-ala carboxypeptidase) PhesterPhotato_20 (minor tail protein) PhesterPhotato_21 PhesterPhotato_22 PhesterPhotato_23 PhesterPhotato_24 PhesterPhotato_25 (helix-turn-helix DNA binding domain) PhesterPhotato_26 PhesterPhotato_27 PhesterPhotato_28 PhesterPhotato_29 PhesterPhotato_30 (lysin A) PhesterPhotato_31 (lysin B) PhesterPhotato_32 (holin) PhesterPhotato_33 PhesterPhotato_34 PhesterPhotato_35 (DnaQ-like DNA polymerase III subunit) PhesterPhotato_36 PhesterPhotato_37 PhesterPhotato_38 PhesterPhotato_39 (hetero-immunity repressor) PhesterPhotato_40 PhesterPhotato_41 (helix-turn-helix DNA binding domain) PhesterPhotato_42 PhesterPhotato_43 PhesterPhotato_44 (tyrosine integrase) PhesterPhotato_45 PhesterPhotato_46 PhesterPhotato_47 (immunity repressor) PhesterPhotato_48 (Cro control of repressor's operator) PhesterPhotato_49 (antirepressor) PhesterPhotato_50 PhesterPhotato_51 PhesterPhotato_52 (excise protein) PhesterPhotato_53 PhesterPhotato_54 PhesterPhotato_55 PhesterPhotato_56 PhesterPhotato_57 PhesterPhotato_58 (WhiB family transcription factor) PhesterPhotato_59 PhesterPhotato_60 (helix-turn-helix DNA binding domain) PhesterPhotato_61 PhesterPhotato_62 PhesterPhotato_63 PhesterPhotato_64 (SSB protein) PhesterPhotato_65 (HNH endonuclease) PhesterPhotato_66 (DNA methyltransferase) PhesterPhotato_67 PhesterPhotato_68 PhesterPhotato_69 (DNA methyltransferase) PhesterPhotato_70 PhesterPhotato_71 (PAPS reductase-like domain) PhesterPhotato_72 PhesterPhotato_73 (DnaQ-like DNA polymerase III subunit) PhesterPhotato_74 PhesterPhotato_75 PhesterPhotato_76 (DNA methyltransferase) PhesterPhotato_77 PhesterPhotato_78 PhesterPhotato_79 PhesterPhotato_80 PhesterPhotato_81 PhesterPhotato_82 PhesterPhotato_83 PhesterPhotato_84 PhesterPhotato_85 PhesterPhotato_86 PhesterPhotato_87 (MPME 1 protein) PhesterPhotato_88 PhesterPhotato_89 PhesterPhotato_90 PhesterPhotato_91 PhesterPhotato_92 PhesterPhotato_93 PhesterPhotato_94 PhesterPhotato_95 PhesterPhotato_96 PhesterPhotato_97 PhesterPhotato_98 (helix-turn-helix DNA binding domain) PhesterPhotato_99 PhesterPhotato_100 PhesterPhotato_101 PhesterPhotato_102 PhesterPhotato_103 PhesterPhotato_104 PhesterPhotato_105 (glycosyltransferase) PhesterPhotato_106 (kinase) PhesterPhotato_107 PhesterPhotato_108 (glycosyltransferase) PhesterPhotato_109 (HNH endonuclease)
Submitted Minimal DNA Master File	Download
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	PP978815
Refseq Number	None yet
Archiving Info
Archiving status	Archived
SEA Lysate Titer	4.2 x 10^10 pfu/mL
Pitt Freezer Box#	186
Pitt Freezer Box Grid#	H12
Available Files
Plaque Picture	Download
Restriction Digest Picture	Download
GenBank File for Phamerator	Download

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