Mycobacterium phage Rita
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Detailed Information for Phage Rita
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByAnna Fakhri
Year Found2021
Location FoundNorth Easton, MA United States
Finding InstitutionProvidence College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature37°C
GPS Coordinates42.051462 N, 71.134382 W Map
Discovery NotesA soil sample was collected in North Easton, Massachusetts, on Friday, May 28th, 2021 at 4:26 pm. The soil was slightly damp and approximately 4 inches deep into the ground, and was found underneath a shaded, grassy area next to an apple tree. The weather was cloudy, with 52% humidity and a temperature of 12 °C.
Naming NotesThe phage name "Rita" is named after my aunt Rita who is one of my biggest role models.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedAug 23, 2021
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage2788
Genome length (bp)58771
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGAAGGCGC
GC Content61.6%
Fasta file available?Yes: Download fasta file
Characterization
ClusterF
SubclusterF1
Cluster Life CycleTemperate
Other Cluster Members

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MulchSalad (None)

Soul22 (None)

Akhila (F1)

Alexphander (F1)

AlpineSix (F1)

ArcusAngelus (F1)

Ardmore (F1)

Aubs (F1)

Awesomesauce (F1)

Batiatus (F1)

Beakin (F1)

BigPhil (F1)

Bipolar (F1)

Blexus (F1)

BlueCrab (F1)

BobaPhett (F1)

Bobi (F1)

BodEinwohner17 (F1)

Boomer (F1)

Brocalys (F1)

Bubbles123 (F1)

Burwell21 (F1)

BuzzLyseyear (F1)

ByChance (F1)

Byougenkin (F1)

Cabrinians (F1)

CaptainTrips (F1)

Cerasum (F1)

Che8 (F1)

ChickenDinner (F1)

Chuckly (F1)

Clifton (F1)

Coco12 (F1)

Cornucopia (F1)

DaddyRickover (F1)

Daenerys (F1)

Dante (F1)

DaWorst (F1)

DeadP (F1)

DillTech15 (F1)

DirtMcgirt (F1)

DLane (F1)

Donkeykong (F1)

Doomphist (F1)

Dorothy (F1)

DotProduct (F1)

Doug (F1)

Drago (F1)

DRBy19 (F1)

Eish (F1)

EleanorGeorge (F1)

Emma (F1)

Empress (F1)

Enby (F1)

Eradicator (F1)

Estave1 (F1)

Fancypants (F1)

Filuzino (F1)

Firehouse51 (F1)

Flathead (F1)

Florinda (F1)

Frankie (F1)

Fruitloop (F1)

Galactic (F1)

Gandalph (F1)

Geralt (F1)

GigiOuiOui (F1)

Girafales (F1)

Girr (F1)

GUmbie (F1)

Hades (F1)

Hamulus (F1)

Harley (F1)

Hegedechwinu (F1)

Hlubikazi (F1)

Ibhubesi (F1)

Inventum (F1)

IrishSherpFalk (F1)

JalFarm20 (F1)

James (F1)

Jarcob (F1)

Job42 (F1)

JoeyJr (F1)

Juice456 (F1)

Karhdo (F1)

Kenuha5 (F1)

Kersh (F1)

Kimberlium (F1)

KingMidas (F1)

Kingsley (F1)

Koella (F1)

Krakatau (F1)

KristaRAM (F1)

LastJedi (F1)

Latretium (F1)

Leozinho (F1)

Leperchaun (F1)

LilMoolah (F1)

Lizziana (F1)

Llama (F1)

Llij (F1)

Lorde (F1)

LunaBlu (F1)

Madiba (F1)

MadMen (F1)

Mahavrat (F1)

Mandlovu (F1)

Mantra (F1)

Martik (F1)

Mattes (F1)

Melissauren88 (F1)

MilanaBonita (F1)

MilleniumForce (F1)

MinionDave (F1)

Minnie (F1)

Misha28 (F1)

MisterCuddles (F1)

Modragons (F1)

Moonbeam (F1)

Mova (F1)

Mozy (F1)

Ms6 (F1)

Mutaforma13 (F1)

Nimbo (F1)

Nitzel (F1)

Nivrat (F1)

NormanBulbieJr (F1)

Ochi17 (F1)

OfUltron (F1)

Ogopogo (F1)

Oksu (F1)

OldBen (F1)

OlympiaSaint (F1)

Ovechkin (F1)

OwlsT2W (F1)

Pacc40 (F1)

Phalconet (F1)

Phanphagia (F1)

PHappiness (F1)

Phasih (F1)

Phatniss (F1)

Piper2020 (F1)

Pippy (F1)

Plumbus (F1)

PMC (F1)

Poenanya (F1)

Polka14 (F1)

Pollywog (F1)

PopTart (F1)

Priscilla (F1)

QuickMath (F1)

Quico (F1)

Radiance (F1)

Ramsey (F1)

RedBird (F1)

Rialto (F1)

Rita (F1)

RitaG (F1)

Rockne (F1)

RockyHorror (F1)

Royals2015 (F1)

Ruby (F1)

Saal (F1)

Sabbb (F1)

Sandalphon (F1)

Sarma624 (F1)

Sassafras (F1)

SassyB (F1)

Scottish (F1)

Seabastian (F1)

Seagreen (F1)

SG4 (F1)

Shauna1 (F1)

ShiLan (F1)

ShroomBoi (F1)

SimranZ1 (F1)

SiSi (F1)

Sparkdehlily (F1)

Spartacus (F1)

Spikelee (F1)

Spoonbill (F1)

StAnnes (F1)

Stap (F1)

Starcevich (F1)

Strokeseat (F1)

SuperGrey (F1)

SwagPigglett (F1)

Taj (F1)

TDanisky (F1)

TootsiePop (F1)

Tootsieroll (F1)

Tweety (F1)

UncleRicky (F1)

Velveteen (F1)

Veteran (F1)

VRedHorse (F1)

Wachhund (F1)

Wee (F1)

Whatsapiecost (F1)

Whouxphf (F1)

WillSterrel (F1)

XFactor (F1)

Yorick (F1)

Zerg (F1)

Zizzle (F1)

Avani (F2)

Che9d (F2)

Demsculpinboyz (F2)

Jabbawokkie (F2)

Yoshi (F2)

Zapner (F2)

Squirty (F3)

Renaud18 (F4)

TChen (F4)

ThetaBob (F4)

Cornie (F5)

Annotating InstitutionProvidence College
Annotation StatusIn GenBank
Plaque NotesThe plaques are very small and clear.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Rita_1
(terminase small subunit)

Rita_2
(terminase large subunit)

Rita_3
(portal protein)

Rita_4
(capsid maturation protease)

Rita_5
(scaffolding protein)

Rita_6
(major capsid protein)

Rita_7
(head-to-tail adaptor)

Rita_8
(head-to-tail stopper)

Rita_9

Rita_10
(tail terminator)

Rita_11
(major tail protein)

Rita_12
(tail assembly chaperone)

Rita_13
(tail assembly chaperone)

Rita_14
(tape measure protein)

Rita_15
(minor tail protein)

Rita_16
(minor tail protein)

Rita_17
(minor tail protein)

Rita_18
(minor tail protein)

Rita_19
(minor tail protein)

Rita_20

Rita_21

Rita_22

Rita_23

Rita_24

Rita_25
(helix-turn-helix DNA binding domain protein)

Rita_26

Rita_27

Rita_28

Rita_29

Rita_30

Rita_31
(lysin A)

Rita_32
(lysin B)

Rita_33
(holin)

Rita_34

Rita_35

Rita_36
(DnaQ-like DNA polymerase III subunit)

Rita_37

Rita_38

Rita_39

Rita_40

Rita_41

Rita_42
(helix-turn-helix DNA binding protein)

Rita_43

Rita_44
(tyrosine integrase)

Rita_45

Rita_46
(immunity repressor)

Rita_47
(Cro protein)

Rita_48

Rita_49

Rita_50

Rita_51

Rita_52
(excise)

Rita_53

Rita_54

Rita_55

Rita_56

Rita_57

Rita_58
(WhiB family transcription factor)

Rita_59

Rita_60
(WhiB family transcription factor)

Rita_61
(helix-turn-helix DNA binding domain protein)

Rita_62

Rita_63

Rita_64

Rita_65
(DNA binding protein)

Rita_66
(HNH endonuclease)

Rita_67
(histidine triad nucleotide binding protein)

Rita_68

Rita_69

Rita_70

Rita_71

Rita_72

Rita_73
(DNA helicase)

Rita_74

Rita_75

Rita_76
(DNA methylase)

Rita_77

Rita_78

Rita_79

Rita_80

Rita_81
(HNH endonuclease)

Rita_82

Rita_83

Rita_84

Rita_85

Rita_86

Rita_87

Rita_88

Rita_89

Rita_90

Rita_91

Rita_92

Rita_93

Rita_94

Rita_95
(exonuclease)

Rita_96

Rita_97

Rita_98

Rita_99

Rita_100

Rita_101
(glycosyltransferase)

Rita_102

Rita_103
(glycosyltransferase)

Rita_104
(HNH endonuclease)

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionOP068340
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 136
Pitt Freezer Box Grid# B6
Available Files
EM PictureDownload
GenBank File for PhameratorDownload