Mycobacterium phage Soul22
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Detailed Information for Phage Soul22
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Former namesSol22, Revolution22
Found ByAlexis Koehl
Year Found2019
Location FoundHolland, MI United States
Finding InstitutionHope College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature18°C
GPS Coordinates42.7697 N, 86.1664 W Map
Discovery NotesCollected on September 12th from compost pile at 8:30 am.
Moved away newer fresh compost and collected underneath.
Heavy rain the night before
Naming NotesNamed because my phage morphology has several halos and it reminded me of the sun rays that form halos around the sun and those are called 22-degree halos.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedFeb 19, 2020
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage876
Genome length (bp)54814
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCTCAGGGCAT
GC Content61.5%
Fasta file available?Yes: Download fasta file
Characterization
ClusterF
Subcluster--
Cluster Life CycleTemperate
Other Cluster Members

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Soul22 (None)

Akhila (F1)

Alexphander (F1)

ArcusAngelus (F1)

Ardmore (F1)

Awesomesauce (F1)

Batiatus (F1)

BigPhil (F1)

Bipolar (F1)

Blexus (F1)

BobaPhett (F1)

Bobi (F1)

BodEinwohner17 (F1)

Boomer (F1)

Brocalys (F1)

Bubbles123 (F1)

Burwell21 (F1)

BuzzLyseyear (F1)

ByChance (F1)

Byougenkin (F1)

Cabrinians (F1)

CaptainTrips (F1)

Cerasum (F1)

Che8 (F1)

Chuckly (F1)

Clifton (F1)

Coco12 (F1)

Cornucopia (F1)

Daenerys (F1)

Dante (F1)

DaWorst (F1)

DeadP (F1)

DillTech15 (F1)

DirtMcgirt (F1)

DLane (F1)

Donkeykong (F1)

Doomphist (F1)

Dorothy (F1)

DotProduct (F1)

Doug (F1)

Drago (F1)

DRBy19 (F1)

Eish (F1)

EleanorGeorge (F1)

Emma (F1)

Empress (F1)

Enby (F1)

Eradicator (F1)

Estave1 (F1)

Fancypants (F1)

Filuzino (F1)

Firehouse51 (F1)

Flathead (F1)

Florinda (F1)

Frankie (F1)

Fruitloop (F1)

Galactic (F1)

Gandalph (F1)

Geralt (F1)

Girafales (F1)

Girr (F1)

Gorge (F1)

GUmbie (F1)

Hades (F1)

Hamulus (F1)

Harley (F1)

Hegedechwinu (F1)

Hlubikazi (F1)

Ibhubesi (F1)

Inventum (F1)

IrishSherpFalk (F1)

James (F1)

Job42 (F1)

JoeyJr (F1)

Juice456 (F1)

Kenuha5 (F1)

Kersh (F1)

Kimberlium (F1)

KingMidas (F1)

Kingsley (F1)

Koella (F1)

Krakatau (F1)

KristaRAM (F1)

LastJedi (F1)

Latretium (F1)

LilMoolah (F1)

Lizziana (F1)

Llama (F1)

Llij (F1)

Lorde (F1)

Mahavrat (F1)

Mandlovu (F1)

Mantra (F1)

Martik (F1)

Mattes (F1)

Melissauren88 (F1)

MilanaBonita (F1)

MilleniumForce (F1)

MinionDave (F1)

Minnie (F1)

Misha28 (F1)

MisterCuddles (F1)

Modragons (F1)

Moonbeam (F1)

Mova (F1)

Mozy (F1)

Mutaforma13 (F1)

Nimbo (F1)

Nitzel (F1)

Nivrat (F1)

NormanBulbieJr (F1)

Ochi17 (F1)

OfUltron (F1)

Ogopogo (F1)

OldBen (F1)

OlympiaSaint (F1)

Ovechkin (F1)

OwlsT2W (F1)

Pacc40 (F1)

Phanphagia (F1)

PHappiness (F1)

Phasih (F1)

Phatniss (F1)

Piper2020 (F1)

Pippy (F1)

Plumbus (F1)

PMC (F1)

Poenanya (F1)

Polka14 (F1)

Pollywog (F1)

PopTart (F1)

Priscilla (F1)

QuickMath (F1)

Quico (F1)

Ramsey (F1)

RitaG (F1)

RockyHorror (F1)

Royals2015 (F1)

Ruby (F1)

Saal (F1)

Sandalphon (F1)

Sassafras (F1)

SassyB (F1)

Scottish (F1)

Seabastian (F1)

Seagreen (F1)

SG4 (F1)

Shauna1 (F1)

ShiLan (F1)

SimranZ1 (F1)

SiSi (F1)

Sparkdehlily (F1)

Spartacus (F1)

Spikelee (F1)

Spoonbill (F1)

StAnnes (F1)

Strokeseat (F1)

SuperGrey (F1)

SwagPigglett (F1)

Taj (F1)

TDanisky (F1)

TootsiePop (F1)

Tweety (F1)

UncleRicky (F1)

Velveteen (F1)

Veteran (F1)

Wachhund (F1)

Wee (F1)

Whouxphf (F1)

WillSterrel (F1)

XFactor (F1)

Zerg (F1)

Avani (F2)

Che9d (F2)

Demsculpinboyz (F2)

Jabbawokkie (F2)

Yoshi (F2)

Zapner (F2)

Squirty (F3)

Renaud18 (F4)

TChen (F4)

ThetaBob (F4)

Cornie (F5)

Annotating InstitutionHope College
Annotation StatusIn GenBank
Plaque NotesThe phage is oval shaped and has a turbid middle with a clear ring around it and it has a turbid ring around it and a turbid tail. It is less than .5 cm in diameter.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Soul22_1
(HNH endonuclease)

Soul22_2
(terminase small subunit)

Soul22_3
(terminase large subunit)

Soul22_4
(portal protein)

Soul22_5
(capsid maturation protease)

Soul22_6
(scaffolding protein)

Soul22_7
(major capsid protein)

Soul22_8

Soul22_9

Soul22_10

Soul22_11

Soul22_12

Soul22_13

Soul22_14
(major tail subunit)

Soul22_15
(tail assembly chaperone)

Soul22_16
(tail assembly chaperone)

Soul22_17
(tape measure protein)

Soul22_18
(minor tail protein)

Soul22_19
(minor tail protein)

Soul22_20
(minor tail protein)

Soul22_21
(minor tail protein)

Soul22_22
(minor tail protein)

Soul22_23
(minor tail protein)

Soul22_24
(minor tail protein)

Soul22_25
(minor tail protein)

Soul22_26
(minor tail protein)

Soul22_27

Soul22_28
(HNH endonuclease)

Soul22_29
(helix-turn-helix DNA binding domain protein)

Soul22_30

Soul22_31

Soul22_32

Soul22_33

Soul22_34
(lysin A)

Soul22_35
(HNH endonuclease)

Soul22_36
(lysin B)

Soul22_37
(holin)

Soul22_38

Soul22_39

Soul22_40
(DNA polymerase III subunit)

Soul22_41

Soul22_42

Soul22_43

Soul22_44

Soul22_45

Soul22_46
(integrase)

Soul22_47

Soul22_48

Soul22_49
(immunity repressor)

Soul22_50
(CRO protein)

Soul22_51
(antirepressor)

Soul22_52

Soul22_53

Soul22_54
(excise)

Soul22_55

Soul22_56

Soul22_57

Soul22_58

Soul22_59

Soul22_60

Soul22_61

Soul22_62

Soul22_63

Soul22_64

Soul22_65
(WhiB transcription factor)

Soul22_66
(helix-turn-helix DNA binding domain protein)

Soul22_67

Soul22_68

Soul22_69

Soul22_70

Soul22_71
(HNH endonuclease)

Soul22_72

Soul22_73

Soul22_74

Soul22_75

Soul22_76

Soul22_77
(G-I-Y Y-I-G endonuclease)

Soul22_78

Soul22_79

Soul22_80

Soul22_81

Soul22_82

Soul22_83

Soul22_84

Soul22_85

Soul22_86

Soul22_87

Soul22_88

Soul22_89

Soul22_90

Soul22_91

Soul22_92

Soul22_93

Soul22_94

Soul22_95
(exonuclease)

Soul22_96

Soul22_97

Soul22_98

Soul22_99

Soul22_100
(glycosyltransferase)

Soul22_101
(glycosyltransferase)

Soul22_102
(glycosyltransferase)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMT522000
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 100
Pitt Freezer Box Grid# A12
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
GenBank File for PhameratorDownload