Detailed Information for Phage Soul22 |
Discovery Information |
Isolation Host | Mycobacterium smegmatis mc²155 |
Former names | Sol22, Revolution22 |
Found By | Alexis Koehl |
Year Found | 2019 |
Location Found | Holland, MI United States |
Finding Institution | Hope College |
Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
From enriched soil sample? | No |
Isolation Temperature | 37°C |
GPS Coordinates | 42.7697 N, 86.1664 W Map |
Discovery Notes | Collected on September 12th from compost pile at 8:30 am. Moved away newer fresh compost and collected underneath. Heavy rain the night before |
Naming Notes | Named because my phage morphology has several halos and it reminded me of the sun rays that form halos around the sun and those are called 22-degree halos. |
Sequencing Information |
Sequencing Complete? | Yes |
Date Sequencing Completed | Feb 19, 2020 |
Sequencing Facility | Pittsburgh Bacteriophage Institute |
Shotgun Sequencing Method | Illumina Sequencing |
Approximate Shotgun Coverage | 876 |
Genome length (bp) | 54814 |
Character of genome ends | 3' Sticky Overhang |
Overhang Length | 10 bases |
Overhang Sequence | CTCAGGGCAT |
GC Content | 61.5% |
Fasta file available? | Yes: Download fasta file |
Characterization |
Cluster | F |
Subcluster | F2 |
Cluster Life Cycle | Temperate |
Other Cluster Members |
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Annotating Institution | Hope College |
Annotation Status | In GenBank |
Plaque Notes | The phage is oval shaped and has a turbid middle with a clear ring around it and it has a turbid ring around it and a turbid tail. It is less than .5 cm in diameter. |
Morphotype | Siphoviridae |
Has been Phamerated? | Yes |
Gene List |
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Submitted Minimal DNA Master File | Download |
Publication Info |
Uploaded to GenBank? | Yes |
GenBank Accession | MT522000 |
Refseq Number | None yet |
Archiving Info |
Archiving status |
Archived |
Pitt Freezer Box# |
100 |
Pitt Freezer Box Grid# |
A12 |
Available Files |
Plaque Picture | Download |
Restriction Digest Picture | Download |
GenBank File for Phamerator | Download |