Mycobacterium phage ByChance
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Detailed Information for Phage ByChance
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByPallavi Kawatra
Year Found2017
Location FoundPhiladelphia, PA USA
Finding InstitutionUniversity of the Sciences in Philadelphia
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates39.9484 N, 75.20905 W Map
Discovery NotesThe soil sample I collected was from the University of the Sciences campus on September 12, 2017 at 6:35pm. I went very deep into the soil, and it was moist. It was around a plant, and there is not much foot traffic there. It is close to buildings, and appears to be taken care of regularly. The soil was very dark and moist.
Naming NotesThe name ByChance was selected because seeing my phage is a very "by chance" type situation. In the beginning, actually seeing the phage was rare; it was there, but very small plaques and very hidden. Even as time went on, ByChance was still rarely seen and is density dependent showing one of the highest concentrations on its fourth dilution. But when you see ByChance, it is a beautiful occasion marked with much joy.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 8, 2018
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1988
Genome length (bp)53123
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGACGGCGC
GC Content61.3%
Fasta file available?Yes: Download fasta file
Characterization
ClusterF
SubclusterF1
Cluster Life CycleTemperate
Other Cluster Members

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Soul22 (None)

Akhila (F1)

Alexphander (F1)

ArcusAngelus (F1)

Ardmore (F1)

Aubs (F1)

Awesomesauce (F1)

Batiatus (F1)

Beakin (F1)

BigPhil (F1)

Bipolar (F1)

Blexus (F1)

BobaPhett (F1)

Bobi (F1)

BodEinwohner17 (F1)

Boomer (F1)

Brocalys (F1)

Bubbles123 (F1)

Burwell21 (F1)

BuzzLyseyear (F1)

ByChance (F1)

Byougenkin (F1)

Cabrinians (F1)

CaptainTrips (F1)

Cerasum (F1)

Che8 (F1)

Chuckly (F1)

Clifton (F1)

Coco12 (F1)

Cornucopia (F1)

Daenerys (F1)

Dante (F1)

DaWorst (F1)

DeadP (F1)

DillTech15 (F1)

DirtMcgirt (F1)

DLane (F1)

Donkeykong (F1)

Doomphist (F1)

Dorothy (F1)

DotProduct (F1)

Doug (F1)

Drago (F1)

DRBy19 (F1)

Eish (F1)

EleanorGeorge (F1)

Emma (F1)

Empress (F1)

Enby (F1)

Eradicator (F1)

Estave1 (F1)

Fancypants (F1)

Filuzino (F1)

Firehouse51 (F1)

Flathead (F1)

Florinda (F1)

Frankie (F1)

Fruitloop (F1)

Galactic (F1)

Gandalph (F1)

Geralt (F1)

GigiOuiOui (F1)

Girafales (F1)

Girr (F1)

Gorge (F1)

GUmbie (F1)

Hades (F1)

Hamulus (F1)

Harley (F1)

Hegedechwinu (F1)

Hlubikazi (F1)

Ibhubesi (F1)

Inventum (F1)

IrishSherpFalk (F1)

JalFarm20 (F1)

James (F1)

Job42 (F1)

JoeyJr (F1)

Juice456 (F1)

Kenuha5 (F1)

Kersh (F1)

Kimberlium (F1)

KingMidas (F1)

Kingsley (F1)

Koella (F1)

Krakatau (F1)

KristaRAM (F1)

LastJedi (F1)

Latretium (F1)

Leozinho (F1)

Leperchaun (F1)

LilMoolah (F1)

Lizziana (F1)

Llama (F1)

Llij (F1)

Lorde (F1)

Madiba (F1)

MadMen (F1)

Mahavrat (F1)

Mandlovu (F1)

Mantra (F1)

Martik (F1)

Mattes (F1)

Melissauren88 (F1)

MilanaBonita (F1)

MilleniumForce (F1)

MinionDave (F1)

Minnie (F1)

Misha28 (F1)

MisterCuddles (F1)

Modragons (F1)

Moonbeam (F1)

Mova (F1)

Mozy (F1)

Mutaforma13 (F1)

Nimbo (F1)

Nitzel (F1)

Nivrat (F1)

NormanBulbieJr (F1)

Ochi17 (F1)

OfUltron (F1)

Ogopogo (F1)

OldBen (F1)

OlympiaSaint (F1)

Ovechkin (F1)

OwlsT2W (F1)

Pacc40 (F1)

Phalconet (F1)

Phanphagia (F1)

PHappiness (F1)

Phasih (F1)

Phatniss (F1)

Piper2020 (F1)

Pippy (F1)

Plumbus (F1)

PMC (F1)

Poenanya (F1)

Polka14 (F1)

Pollywog (F1)

PopTart (F1)

Priscilla (F1)

QuickMath (F1)

Quico (F1)

Ramsey (F1)

Rita (F1)

RitaG (F1)

Rockne (F1)

RockyHorror (F1)

Royals2015 (F1)

Ruby (F1)

Saal (F1)

Sabbb (F1)

Sandalphon (F1)

Sarma624 (F1)

Sassafras (F1)

SassyB (F1)

Scottish (F1)

Seabastian (F1)

Seagreen (F1)

SG4 (F1)

Shauna1 (F1)

ShiLan (F1)

SimranZ1 (F1)

SiSi (F1)

Sparkdehlily (F1)

Spartacus (F1)

Spikelee (F1)

Spoonbill (F1)

StAnnes (F1)

Strokeseat (F1)

SuperGrey (F1)

SwagPigglett (F1)

Taj (F1)

TDanisky (F1)

TootsiePop (F1)

Tootsieroll (F1)

Tweety (F1)

UncleRicky (F1)

Velveteen (F1)

Veteran (F1)

VRedHorse (F1)

Wachhund (F1)

Wee (F1)

Whatsapiecost (F1)

Whouxphf (F1)

WillSterrel (F1)

XFactor (F1)

Yorick (F1)

Zerg (F1)

Zizzle (F1)

Avani (F2)

Che9d (F2)

Demsculpinboyz (F2)

Jabbawokkie (F2)

Yoshi (F2)

Zapner (F2)

Squirty (F3)

Renaud18 (F4)

TChen (F4)

ThetaBob (F4)

Cornie (F5)

Annotating InstitutionUniversity of the Sciences in Philadelphia
Annotation StatusIn GenBank
Plaque NotesThe phage appears to be lytic as there are clear plaques on the plates.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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ByChance_1
(terminase small subunit)

ByChance_2
(terminase large subunit)

ByChance_3
(portal protein)

ByChance_4

ByChance_5
(scaffolding protein)

ByChance_6
(major capsid protein)

ByChance_7
(head-to-tail adaptor)

ByChance_8
(head-to-tail stopper)

ByChance_9

ByChance_10
(tail terminator)

ByChance_11
(major tail protein)

ByChance_12
(tail assembly chaperone)

ByChance_13
(tail assembly chaperone)

ByChance_14
(tape measure protein)

ByChance_15
(minor tail protein)

ByChance_16
(minor tail protein)

ByChance_17
(minor tail protein)

ByChance_18
(minor tail protein)

ByChance_19
(minor tail protein)

ByChance_20
(minor tail protein)

ByChance_21

ByChance_22

ByChance_23

ByChance_24

ByChance_25

ByChance_26

ByChance_27

ByChance_28

ByChance_29

ByChance_30
(lysin A)

ByChance_31

ByChance_32

ByChance_33

ByChance_34

ByChance_35

ByChance_36
(helix-turn-helix DNA binding domain protein)

ByChance_37

ByChance_38

ByChance_39

ByChance_40

ByChance_41

ByChance_42
(helix-turn-helix DNA binding domain protein)

ByChance_43

ByChance_44

ByChance_45

ByChance_46

ByChance_47

ByChance_48

ByChance_49
(WhiB family transcription factor)

ByChance_50
(helix-turn-helix DNA binding domain protein)

ByChance_51
(WhiB family transcription factor)

ByChance_52
(helix-turn-helix DNA binding domain protein)

ByChance_53

ByChance_54

ByChance_55

ByChance_56

ByChance_57
(RecA-like DNA recombinase)

ByChance_58
(HNH endonuclease)

ByChance_59
(DNA helicase)

ByChance_60
(DNA methylase)

ByChance_61

ByChance_62

ByChance_63
(DNA methylase)

ByChance_64
(DNA methylase)

ByChance_65

ByChance_66

ByChance_67
(helix-turn-helix DNA binding domain protein)

ByChance_68

ByChance_69

ByChance_70

ByChance_71

ByChance_72

ByChance_73

ByChance_74

ByChance_75

ByChance_76

ByChance_77

ByChance_78

ByChance_79

ByChance_80

ByChance_81
(G-I-Y Y-I-G endonuclease)

ByChance_82

ByChance_83

ByChance_84

ByChance_85

ByChance_86

ByChance_87

ByChance_88

ByChance_89

ByChance_90

ByChance_91

ByChance_92
(glycosyltransferase)

ByChance_93

ByChance_94
(glycosyltransferase)

ByChance_95
(HNH endonuclease)

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMH399771
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 47
Pitt Freezer Box Grid# B8
Available Files
Plaque PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload