Mycobacterium phage Karhdo
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Detailed Information for Phage Karhdo
Discovery Information
Isolation HostMycobacterium smegmatis ATCC 607
Found ByKhristian Rosales
Year Found2022
Location FoundLas Vegas, NV United States
Finding InstitutionUniversity of Nevada Las Vegas
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature27°C
GPS Coordinates36.131401 N, 115.240075 W Map
Discovery NotesOrganic garden soil. The soil was recovered from 4-6 inches deep in the dirt.
Naming NotesThe name came from a combination of initials from my personal name.

K-hristian A-drian R-osales
KAR
K-h-ristian A-d-rian R-o-sales
HDO

=KARHDO
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 9, 2023
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage3421
Genome length (bp)55379
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGTAGGCGC
GC Content61.5%
Fasta file available?Yes: Download fasta file
Characterization
ClusterF
SubclusterF1
Cluster Life CycleTemperate
Other Cluster Members

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MulchSalad (None)

Soul22 (None)

Akhila (F1)

Alexphander (F1)

AlpineSix (F1)

ArcusAngelus (F1)

Ardmore (F1)

Aubs (F1)

Awesomesauce (F1)

Batiatus (F1)

Beakin (F1)

BigPhil (F1)

Bipolar (F1)

Blexus (F1)

BlueCrab (F1)

BobaPhett (F1)

Bobi (F1)

BodEinwohner17 (F1)

Boomer (F1)

Brocalys (F1)

Bubbles123 (F1)

Burwell21 (F1)

BuzzLyseyear (F1)

ByChance (F1)

Byougenkin (F1)

Cabrinians (F1)

CaptainTrips (F1)

Cerasum (F1)

Che8 (F1)

ChickenDinner (F1)

Chuckly (F1)

Clifton (F1)

Coco12 (F1)

Cornucopia (F1)

DaddyRickover (F1)

Daenerys (F1)

Dante (F1)

DaWorst (F1)

DeadP (F1)

DillTech15 (F1)

DirtMcgirt (F1)

DLane (F1)

Donkeykong (F1)

Doomphist (F1)

Dorothy (F1)

DotProduct (F1)

Doug (F1)

Drago (F1)

DRBy19 (F1)

Eish (F1)

EleanorGeorge (F1)

Emma (F1)

Empress (F1)

Enby (F1)

Eradicator (F1)

Estave1 (F1)

Fancypants (F1)

Filuzino (F1)

Firehouse51 (F1)

Flathead (F1)

Florinda (F1)

Frankie (F1)

Fruitloop (F1)

Galactic (F1)

Gandalph (F1)

Geralt (F1)

GigiOuiOui (F1)

Girafales (F1)

Girr (F1)

GUmbie (F1)

Hades (F1)

Hamulus (F1)

Harley (F1)

Hegedechwinu (F1)

Hlubikazi (F1)

Ibhubesi (F1)

Inventum (F1)

IrishSherpFalk (F1)

JalFarm20 (F1)

James (F1)

Jarcob (F1)

Job42 (F1)

JoeyJr (F1)

Juice456 (F1)

Karhdo (F1)

Kenuha5 (F1)

Kersh (F1)

Kimberlium (F1)

KingMidas (F1)

Kingsley (F1)

Koella (F1)

Krakatau (F1)

KristaRAM (F1)

LastJedi (F1)

Latretium (F1)

Leozinho (F1)

Leperchaun (F1)

LilMoolah (F1)

Lizziana (F1)

Llama (F1)

Llij (F1)

Lorde (F1)

LunaBlu (F1)

Madiba (F1)

MadMen (F1)

Mahavrat (F1)

Mandlovu (F1)

Mantra (F1)

Maravista (F1)

Martik (F1)

Mattes (F1)

Melissauren88 (F1)

MilanaBonita (F1)

MilleniumForce (F1)

MinionDave (F1)

Minnie (F1)

Misha28 (F1)

MisterCuddles (F1)

Modragons (F1)

Moonbeam (F1)

Mova (F1)

Mozy (F1)

Ms6 (F1)

Mutaforma13 (F1)

Nimbo (F1)

Nitzel (F1)

Nivrat (F1)

NormanBulbieJr (F1)

Ochi17 (F1)

OfUltron (F1)

Ogopogo (F1)

Oksu (F1)

OldBen (F1)

OlympiaSaint (F1)

Ovechkin (F1)

OwlsT2W (F1)

Pacc40 (F1)

Phalconet (F1)

Phanphagia (F1)

PHappiness (F1)

Phasih (F1)

Phatniss (F1)

Philus (F1)

Piper2020 (F1)

Pippy (F1)

Plumbus (F1)

PMC (F1)

Poenanya (F1)

Polka14 (F1)

Pollywog (F1)

PopTart (F1)

Priscilla (F1)

QuickMath (F1)

Quico (F1)

Radiance (F1)

Ramsey (F1)

RedBird (F1)

Rialto (F1)

Rita (F1)

RitaG (F1)

Rockne (F1)

RockyHorror (F1)

Royals2015 (F1)

Ruby (F1)

Saal (F1)

Sabbb (F1)

Sandalphon (F1)

Sarma624 (F1)

Sassafras (F1)

SassyB (F1)

Scottish (F1)

Seabastian (F1)

Seagreen (F1)

SG4 (F1)

Shauna1 (F1)

ShiLan (F1)

ShroomBoi (F1)

SimranZ1 (F1)

SiSi (F1)

Sparkdehlily (F1)

Spartacus (F1)

Spikelee (F1)

Spoonbill (F1)

StAnnes (F1)

Stap (F1)

Starcevich (F1)

Strokeseat (F1)

SuperGrey (F1)

SwagPigglett (F1)

Taj (F1)

TDanisky (F1)

TootsiePop (F1)

Tootsieroll (F1)

Tweety (F1)

UncleRicky (F1)

Velveteen (F1)

Veteran (F1)

VRedHorse (F1)

Wachhund (F1)

Wee (F1)

Whatsapiecost (F1)

Whouxphf (F1)

WillSterrel (F1)

XFactor (F1)

Yorick (F1)

Zerg (F1)

Zizzle (F1)

Avani (F2)

Che9d (F2)

Demsculpinboyz (F2)

Jabbawokkie (F2)

Yoshi (F2)

Zapner (F2)

Squirty (F3)

Renaud18 (F4)

TChen (F4)

ThetaBob (F4)

Cornie (F5)

Annotating InstitutionUniversity of Nevada Las Vegas
Annotation StatusBeing Annotated (Expected completion by 5/1/2023)
Plaque NotesThe plaques are cloudy. Have a diameter of 0.5. The outer edges have an irregular circular shape to them. Lysogen can be seen in the center of the plaques.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 162
Pitt Freezer Box Grid# G7
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload