Mycobacterium phage Karhdo
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Detailed Information for Phage Karhdo
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByKhristian Rosales
Year Found2022
Location FoundLas Vegas, NV United States
Finding InstitutionUniversity of Nevada Las Vegas
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature27°C
GPS Coordinates36.131376 N, 115.240078 W Map
Discovery NotesOrganic garden soil. The soil was recovered from 4-6 inches deep in the dirt.
Naming NotesThe name came from a combination of initials from my personal name.

K-hristian A-drian R-osales
KAR
K-h-ristian A-d-rian R-o-sales
HDO

=KARHDO
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 9, 2023
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage3421
Genome length (bp)55379
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGTAGGCGC
GC Content61.5%
Fasta file available?Yes: Download fasta file
Characterization
ClusterF
SubclusterF1
Cluster Life CycleTemperate
Other Cluster Members

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Blarby (None)

MulchSalad (None)

Akhila (F1)

Alexphander (F1)

AlpineSix (F1)

ArcusAngelus (F1)

Ardmore (F1)

Aubs (F1)

Awesomesauce (F1)

Batiatus (F1)

Beakin (F1)

BigPhil (F1)

Bipolar (F1)

Blexus (F1)

BlueCrab (F1)

BobaPhett (F1)

Bobi (F1)

BodEinwohner17 (F1)

Boomer (F1)

Brocalys (F1)

Bubbles123 (F1)

Burwell21 (F1)

BuzzLyseyear (F1)

ByChance (F1)

Byougenkin (F1)

Cabrinians (F1)

CaptainTrips (F1)

Cerasum (F1)

Che8 (F1)

Chevrolet (F1)

ChickenDinner (F1)

Chuckly (F1)

Clifton (F1)

Coco12 (F1)

Cornucopia (F1)

Crumble (F1)

DaddyRickover (F1)

Daenerys (F1)

Dante (F1)

DaWorst (F1)

DeadP (F1)

Deb65 (F1)

DillTech15 (F1)

DirtMcgirt (F1)

DLane (F1)

DocMcStuffins (F1)

Donkeykong (F1)

Doomphist (F1)

Dorothy (F1)

DosHalletts (F1)

DotProduct (F1)

Doug (F1)

Drago (F1)

DRBy19 (F1)

Eish (F1)

EleanorGeorge (F1)

Emma (F1)

Empress (F1)

Enby (F1)

Eradicator (F1)

Estave1 (F1)

Fancypants (F1)

Filuzino (F1)

Firehouse51 (F1)

Flathead (F1)

Florinda (F1)

Frankie (F1)

FreddyB (F1)

Fruitloop (F1)

Galactic (F1)

Gandalph (F1)

Geralt (F1)

GigiOuiOui (F1)

Girafales (F1)

Girr (F1)

Gorge (F1)

GUmbie (F1)

Guppsters (F1)

Hades (F1)

Hamulus (F1)

Harley (F1)

Hegedechwinu (F1)

Hlubikazi (F1)

Ibhubesi (F1)

IbOuu (F1)

Inventum (F1)

IrishSherpFalk (F1)

JalFarm20 (F1)

James (F1)

Jarcob (F1)

Jinglebell (F1)

Job42 (F1)

JoeyJr (F1)

Juice456 (F1)

Juniper1 (F1)

Karhdo (F1)

Kenuha5 (F1)

Kersh (F1)

Kimberlium (F1)

KingMidas (F1)

Kingsley (F1)

Koella (F1)

Krakatau (F1)

KristaRAM (F1)

LastJedi (F1)

Latretium (F1)

Leozinho (F1)

Leperchaun (F1)

LilMoolah (F1)

LittleShirley (F1)

Lizziana (F1)

Llama (F1)

Llij (F1)

Lorde (F1)

LunaBlu (F1)

Madiba (F1)

MadMen (F1)

Mahavrat (F1)

Mandlovu (F1)

Mantra (F1)

Maravista (F1)

Marker (F1)

Martik (F1)

Mattes (F1)

Melissauren88 (F1)

MilanaBonita (F1)

MilleniumForce (F1)

MinionDave (F1)

Minnie (F1)

Misha28 (F1)

MisterCuddles (F1)

Modragons (F1)

Moonbeam (F1)

Mova (F1)

Mozy (F1)

Ms6 (F1)

Mutaforma13 (F1)

Nimbo (F1)

Nitzel (F1)

Nivrat (F1)

NormanBulbieJr (F1)

Ochi17 (F1)

OfUltron (F1)

Ogopogo (F1)

Oksu (F1)

OldBen (F1)

OlympiaSaint (F1)

Ovechkin (F1)

OwlsT2W (F1)

Pacc40 (F1)

Phalconet (F1)

Phanphagia (F1)

PHappiness (F1)

Phasih (F1)

Phatniss (F1)

PhesterPhotato (F1)

Philus (F1)

Piper2020 (F1)

Pippy (F1)

Plumbus (F1)

PMC (F1)

Poenanya (F1)

Polka14 (F1)

Pollywog (F1)

PopTart (F1)

Priscilla (F1)

QuickMath (F1)

Quico (F1)

Radiance (F1)

Ramsey (F1)

RedBird (F1)

Rialto (F1)

Rita (F1)

RitaG (F1)

Rockne (F1)

RockyHorror (F1)

Royals2015 (F1)

Ruby (F1)

Saal (F1)

Sabbb (F1)

Sandalphon (F1)

Sarma624 (F1)

Sassafras (F1)

SassyB (F1)

Scottish (F1)

Seabastian (F1)

Seagreen (F1)

SG4 (F1)

Shauna1 (F1)

ShiLan (F1)

ShowerHandel (F1)

ShroomBoi (F1)

SimranZ1 (F1)

SiSi (F1)

Sparkdehlily (F1)

Spartacus (F1)

Spikelee (F1)

Spoonbill (F1)

StAnnes (F1)

Stap (F1)

Starcevich (F1)

Strokeseat (F1)

SuperGrey (F1)

SwagPigglett (F1)

Taj (F1)

TDanisky (F1)

TootsiePop (F1)

Tootsieroll (F1)

Totinger (F1)

Tweety (F1)

UncleRicky (F1)

Velveteen (F1)

Veteran (F1)

VRedHorse (F1)

Wachhund (F1)

Wee (F1)

Whatsapiecost (F1)

Whouxphf (F1)

WillSterrel (F1)

XFactor (F1)

Yorick (F1)

Zerg (F1)

Zizzle (F1)

Avani (F2)

Che9d (F2)

Demsculpinboyz (F2)

Jabbawokkie (F2)

Soul22 (F2)

Yoshi (F2)

Zapner (F2)

Squirty (F3)

LunaStella (F4)

Renaud18 (F4)

TChen (F4)

ThetaBob (F4)

Cornie (F5)

Annotating InstitutionUniversity of Nevada Las Vegas
Annotation StatusIn GenBank
Plaque NotesThe plaques are cloudy. Have a diameter of 0.5. The outer edges have an irregular circular shape to them. Lysogen can be seen in the center of the plaques.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Karhdo_1
(terminase small subunit)

Karhdo_2
(terminase large subunit)

Karhdo_3
(portal protein)

Karhdo_4
(capsid maturation protease)

Karhdo_5
(scaffolding protein)

Karhdo_6
(major capsid protein)

Karhdo_7
(head-to-tail adaptor)

Karhdo_8
(head-to-tail stopper)

Karhdo_9

Karhdo_10
(tail terminator)

Karhdo_11
(major tail protein)

Karhdo_12
(tail assembly chaperone)

Karhdo_13
(tail assembly chaperone)

Karhdo_14
(tape measure protein)

Karhdo_15
(minor tail protein)

Karhdo_16
(minor tail protein)

Karhdo_17
(minor tail protein)

Karhdo_18
(minor tail protein)

Karhdo_19
(minor tail protein)

Karhdo_20
(minor tail protein)

Karhdo_21
(minor tail protein)

Karhdo_22
(minor tail protein)

Karhdo_23
(minor tail protein)

Karhdo_24

Karhdo_25

Karhdo_26

Karhdo_27

Karhdo_28

Karhdo_29

Karhdo_30
(lysin A)

Karhdo_31
(lysin B)

Karhdo_32
(holin)

Karhdo_33

Karhdo_34

Karhdo_35
(DnaQ-like DNA polymerase III subunit)

Karhdo_36

Karhdo_37

Karhdo_38

Karhdo_39
(helix-turn-helix DNA binding domain protein)

Karhdo_40

Karhdo_41

Karhdo_42
(tyrosine integrase)

Karhdo_43

Karhdo_44
(immunity repressor)

Karhdo_45

Karhdo_46
(antirepressor)

Karhdo_47

Karhdo_48

Karhdo_49
(excise)

Karhdo_50

Karhdo_51

Karhdo_52

Karhdo_53

Karhdo_54
(WhiB family transcription factor)

Karhdo_55

Karhdo_56
(WhiB family transcription factor)

Karhdo_57
(helix-turn-helix DNA binding domain protein)

Karhdo_58

Karhdo_59

Karhdo_60

Karhdo_61
(AAA-ATPase)

Karhdo_62

Karhdo_63

Karhdo_64
(HNH endonuclease)

Karhdo_65

Karhdo_66

Karhdo_67

Karhdo_68

Karhdo_69

Karhdo_70

Karhdo_71

Karhdo_72
(RepA-like replication initiator)

Karhdo_73

Karhdo_74

Karhdo_75

Karhdo_76

Karhdo_77

Karhdo_78

Karhdo_79

Karhdo_80

Karhdo_81

Karhdo_82

Karhdo_83

Karhdo_84

Karhdo_85

Karhdo_86

Karhdo_87

Karhdo_88

Karhdo_89
(G-I-Y Y-I-G endonuclease)

Karhdo_90

Karhdo_91

Karhdo_92

Karhdo_93

Karhdo_94

Karhdo_95

Karhdo_96

Karhdo_97
(glycosyltransferase)

Karhdo_98

Karhdo_99
(glycosyltransferase)

Karhdo_100
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionOR159669
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 162
Pitt Freezer Box Grid# G7
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload