Mycobacterium phage Minnie
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Detailed Information for Phage Minnie
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByAlison Rhodes
Year Found2013
Location FoundHolland, MI USA
Finding InstitutionHope College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Unknown
Isolation TemperatureNot entered
GPS Coordinates42.787243, -86.102250 Map
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedDec 9, 2013
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage2691
Genome length (bp)59268
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGAAGGCGC
GC Content61.5%
Fasta file available?Yes: Download fasta file
Characterization
ClusterF
SubclusterF1
Cluster Life CycleTemperate
Other Cluster Members

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Renaud18 (None)

Alexphander (F1)

ArcusAngelus (F1)

Ardmore (F1)

Awesomesauce (F1)

Batiatus (F1)

BigPhil (F1)

Bipolar (F1)

Blexus (F1)

BobaPhett (F1)

Bobi (F1)

BodEinwohner17 (F1)

Boomer (F1)

Brocalys (F1)

Bubbles123 (F1)

Burwell21 (F1)

BuzzLyseyear (F1)

ByChance (F1)

Byougenkin (F1)

Cabrinians (F1)

CaptainTrips (F1)

Cerasum (F1)

Che8 (F1)

Chuckly (F1)

Clifton (F1)

Cornucopia (F1)

Daenerys (F1)

Dante (F1)

DaWorst (F1)

DeadP (F1)

DillTech15 (F1)

DirtMcgirt (F1)

DLane (F1)

Donkeykong (F1)

Doomphist (F1)

Dorothy (F1)

DotProduct (F1)

Doug (F1)

Drago (F1)

DRBy19 (F1)

Eish (F1)

EleanorGeorge (F1)

Emma (F1)

Empress (F1)

Enby (F1)

Estave1 (F1)

Fancypants (F1)

Filuzino (F1)

Firehouse51 (F1)

Flathead (F1)

Florinda (F1)

Frankie (F1)

Fruitloop (F1)

Galactic (F1)

Geralt (F1)

Girafales (F1)

Girr (F1)

Gorge (F1)

GUmbie (F1)

Hades (F1)

Hamulus (F1)

Harley (F1)

Hegedechwinu (F1)

Ibhubesi (F1)

Inventum (F1)

IrishSherpFalk (F1)

James (F1)

Job42 (F1)

JoeyJr (F1)

Juice456 (F1)

Kenuha5 (F1)

Kersh (F1)

Kimberlium (F1)

KingMidas (F1)

Kingsley (F1)

Koella (F1)

Krakatau (F1)

KristaRAM (F1)

LastJedi (F1)

Latretium (F1)

LilMoolah (F1)

Lizziana (F1)

Llama (F1)

Llij (F1)

Lorde (F1)

Mahavrat (F1)

Mantra (F1)

Mattes (F1)

Melissauren88 (F1)

MilleniumForce (F1)

MinionDave (F1)

Minnie (F1)

Misha28 (F1)

MisterCuddles (F1)

Modragons (F1)

Mozy (F1)

Mutaforma13 (F1)

Nimbo (F1)

Nitzel (F1)

Nivrat (F1)

NormanBulbieJr (F1)

Ochi17 (F1)

OfUltron (F1)

Ogopogo (F1)

OldBen (F1)

OlympiaSaint (F1)

Ovechkin (F1)

OwlsT2W (F1)

Pacc40 (F1)

Phanphagia (F1)

PHappiness (F1)

Phasih (F1)

Phatniss (F1)

Piper2020 (F1)

Pippy (F1)

Plumbus (F1)

PMC (F1)

Poenanya (F1)

Polka14 (F1)

Pollywog (F1)

PopTart (F1)

Priscilla (F1)

QuickMath (F1)

Quico (F1)

Ramsey (F1)

RitaG (F1)

RockyHorror (F1)

Royals2015 (F1)

Ruby (F1)

Saal (F1)

Sandalphon (F1)

SassyB (F1)

Scottish (F1)

Seabastian (F1)

Seagreen (F1)

SG4 (F1)

Shauna1 (F1)

ShiLan (F1)

SimranZ1 (F1)

SiSi (F1)

Sparkdehlily (F1)

Spartacus (F1)

Spikelee (F1)

Spoonbill (F1)

StAnnes (F1)

Strokeseat (F1)

SuperGrey (F1)

SwagPigglett (F1)

Taj (F1)

TDanisky (F1)

TootsiePop (F1)

Tweety (F1)

UncleRicky (F1)

Velveteen (F1)

Veteran (F1)

Wachhund (F1)

Wee (F1)

Whouxphf (F1)

WillSterrel (F1)

XFactor (F1)

Zerg (F1)

Avani (F2)

Che9d (F2)

Demsculpinboyz (F2)

Jabbawokkie (F2)

Yoshi (F2)

Zapner (F2)

Squirty (F3)

TChen (F4)

ThetaBob (F4)

Cornie (F5)

Annotating InstitutionFlorida International University
Annotation StatusQC Complete
Plaque NotesTemperate phage
0.1-0.2 cm plaque size
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Minnie_1
(terminase, small subunit)

Minnie_2
(terminase, large subunit)

Minnie_3
(portal protein)

Minnie_4
(capsid maturation protease and MuF-like fusion protein)

Minnie_5
(scaffolding protein)

Minnie_6
(major capsid protein)

Minnie_7
(head-to-tail adaptor)

Minnie_8
(head-to-tail stopper)

Minnie_9

Minnie_10
(tail terminator)

Minnie_11
(major tail protein)

Minnie_12
(tail assembly chaperone)

Minnie_13
(tail assembly chaperone)

Minnie_14
(HNH endonuclease)

Minnie_15
(tape measure protein)

Minnie_16
(minor tail protein)

Minnie_17
(minor tail protein)

Minnie_18

Minnie_19
(minor tail protein)

Minnie_20
(minor tail protein, D-ala-D-ala carboxypeptidase)

Minnie_21
(minor tail protein)

Minnie_22

Minnie_23

Minnie_24

Minnie_25

Minnie_26
(helix-turn-helix dna binding domain)

Minnie_27
(helix-turn-helix dna binding domain)

Minnie_28

Minnie_29
(membrane protein)

Minnie_30

Minnie_31

Minnie_32
(lysin A)

Minnie_33
(lysin B)

Minnie_34

Minnie_35
(membrane protein)

Minnie_36

Minnie_37
(DnaQ-like (DNA polymerase III subunit))

Minnie_38

Minnie_39
(membrane protein)

Minnie_40

Minnie_41

Minnie_42

Minnie_43
(tyrosine integrase)

Minnie_44
(toxin in toxin/antitoxin system, RelE-like)

Minnie_45
(antitoxin in toxin/antitoxin system, RelB-like)

Minnie_46
(membrane protein)

Minnie_47

Minnie_48
(immunity repressor)

Minnie_49
(Cro (control of repressor))

Minnie_50
(antirepressor)

Minnie_51

Minnie_52

Minnie_53

Minnie_54

Minnie_55
(excise)

Minnie_56

Minnie_57
(membrane protein)

Minnie_58

Minnie_59

Minnie_60

Minnie_61
(WhiB family transcription factor)

Minnie_62
(helix-turn-helix DNA binding domain)

Minnie_63
(helix-turn-helix DNA binding domain, MerR-like)

Minnie_64

Minnie_65

Minnie_66
(Ku-like dsDNA break-binding protein)

Minnie_67
(AAA-ATPase)

Minnie_68
(HNH endonuclease)

Minnie_69
(DNA methylase)

Minnie_70

Minnie_71

Minnie_72
(helix-turn-helix dna binding domain)

Minnie_73

Minnie_74

Minnie_75

Minnie_76

Minnie_77

Minnie_78

Minnie_79

Minnie_80
(membrane protein)

Minnie_81

Minnie_82

Minnie_83

Minnie_84

Minnie_85
(G-I-Y Y-I-G endonuclease)

Minnie_86
(ribbon-helix-helix DNA binding domain)

Minnie_87
(mycobacteriophage mobile element 2 (MPME 2))

Minnie_88

Minnie_89
(membrane protein)

Minnie_90

Minnie_91

Minnie_92

Minnie_93

Minnie_94

Minnie_95

Minnie_96
(glycosyltransferase)

Minnie_97
(Hypotheical Protein)

Minnie_98
(glycosyltransferase)

Minnie_99
(HNH endonuclease)

Minnie_100
(HNH endonuclease)

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Published in PaperPope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status Archived
SEA Lysate Titer 7x10^10
Pitt Freezer Box# 5
Pitt Freezer Box Grid# B4
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
GenBank File for PhameratorDownload