Mycobacterium phage Gandalph
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Detailed Information for Phage Gandalph
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByEmily Carlson
Year Found2011
Location FoundSpokane, WA USA
Finding InstitutionGonzaga University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature37°C
GPS Coordinates47.667944 N, 117.404394 W Map
Discovery NotesGandalph was captured on September 2nd 2011, in a moist flowerbed, underneath a pink rosebush behind St Aloysius Church in Spokane, WA. The three-tablespoon sample of soil was collected at 2 inches and the temperature was 69°F. The soil itself was chunky and moist. The environment was at an elevation of 1913ft.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedAug 28, 2019
Sequencing FacilityNC State Genomic Sciences Laboratory
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage9042
Genome length (bp)54974
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGACGGCGC
GC Content61.4%
Fasta file available?Yes: Download fasta file
Characterization
ClusterF
SubclusterF1
Cluster Life CycleTemperate
Other Cluster Members

Click to View

Soul22 (None)

Akhila (F1)

Alexphander (F1)

ArcusAngelus (F1)

Ardmore (F1)

Awesomesauce (F1)

Batiatus (F1)

BigPhil (F1)

Bipolar (F1)

Blexus (F1)

BobaPhett (F1)

Bobi (F1)

BodEinwohner17 (F1)

Boomer (F1)

Brocalys (F1)

Bubbles123 (F1)

Burwell21 (F1)

BuzzLyseyear (F1)

ByChance (F1)

Byougenkin (F1)

Cabrinians (F1)

CaptainTrips (F1)

Cerasum (F1)

Che8 (F1)

Chuckly (F1)

Clifton (F1)

Coco12 (F1)

Cornucopia (F1)

Daenerys (F1)

Dante (F1)

DaWorst (F1)

DeadP (F1)

DillTech15 (F1)

DirtMcgirt (F1)

DLane (F1)

Donkeykong (F1)

Doomphist (F1)

Dorothy (F1)

DotProduct (F1)

Doug (F1)

Drago (F1)

DRBy19 (F1)

Eish (F1)

EleanorGeorge (F1)

Emma (F1)

Empress (F1)

Enby (F1)

Eradicator (F1)

Estave1 (F1)

Fancypants (F1)

Filuzino (F1)

Firehouse51 (F1)

Flathead (F1)

Florinda (F1)

Frankie (F1)

Fruitloop (F1)

Galactic (F1)

Gandalph (F1)

Geralt (F1)

Girafales (F1)

Girr (F1)

Gorge (F1)

GUmbie (F1)

Hades (F1)

Hamulus (F1)

Harley (F1)

Hegedechwinu (F1)

Hlubikazi (F1)

Ibhubesi (F1)

Inventum (F1)

IrishSherpFalk (F1)

James (F1)

Job42 (F1)

JoeyJr (F1)

Juice456 (F1)

Kenuha5 (F1)

Kersh (F1)

Kimberlium (F1)

KingMidas (F1)

Kingsley (F1)

Koella (F1)

Krakatau (F1)

KristaRAM (F1)

LastJedi (F1)

Latretium (F1)

LilMoolah (F1)

Lizziana (F1)

Llama (F1)

Llij (F1)

Lorde (F1)

Mahavrat (F1)

Mandlovu (F1)

Mantra (F1)

Martik (F1)

Mattes (F1)

Melissauren88 (F1)

MilanaBonita (F1)

MilleniumForce (F1)

MinionDave (F1)

Minnie (F1)

Misha28 (F1)

MisterCuddles (F1)

Modragons (F1)

Moonbeam (F1)

Mova (F1)

Mozy (F1)

Mutaforma13 (F1)

Nimbo (F1)

Nitzel (F1)

Nivrat (F1)

NormanBulbieJr (F1)

Ochi17 (F1)

OfUltron (F1)

Ogopogo (F1)

OldBen (F1)

OlympiaSaint (F1)

Ovechkin (F1)

OwlsT2W (F1)

Pacc40 (F1)

Phanphagia (F1)

PHappiness (F1)

Phasih (F1)

Phatniss (F1)

Piper2020 (F1)

Pippy (F1)

Plumbus (F1)

PMC (F1)

Poenanya (F1)

Polka14 (F1)

Pollywog (F1)

PopTart (F1)

Priscilla (F1)

QuickMath (F1)

Quico (F1)

Ramsey (F1)

RitaG (F1)

RockyHorror (F1)

Royals2015 (F1)

Ruby (F1)

Saal (F1)

Sandalphon (F1)

Sassafras (F1)

SassyB (F1)

Scottish (F1)

Seabastian (F1)

Seagreen (F1)

SG4 (F1)

Shauna1 (F1)

ShiLan (F1)

SimranZ1 (F1)

SiSi (F1)

Sparkdehlily (F1)

Spartacus (F1)

Spikelee (F1)

Spoonbill (F1)

StAnnes (F1)

Strokeseat (F1)

SuperGrey (F1)

SwagPigglett (F1)

Taj (F1)

TDanisky (F1)

TootsiePop (F1)

Tweety (F1)

UncleRicky (F1)

Velveteen (F1)

Veteran (F1)

Wachhund (F1)

Wee (F1)

Whouxphf (F1)

WillSterrel (F1)

XFactor (F1)

Zerg (F1)

Avani (F2)

Che9d (F2)

Demsculpinboyz (F2)

Jabbawokkie (F2)

Yoshi (F2)

Zapner (F2)

Squirty (F3)

Renaud18 (F4)

TChen (F4)

ThetaBob (F4)

Cornie (F5)

Annotating InstitutionGonzaga University
Annotation StatusIn GenBank
Plaque NotesAn image of the 10^-6 dilution of the HTL of Gandalph showing the plaque morphology. The plaques are small with clear edges. The white swirls are colonies of M. smegmatis.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Gandalph_1
(terminase, small subunit)

Gandalph_2
(terminase, large subunit)

Gandalph_3
(portal protein)

Gandalph_4
(MuF-like minor capsid protein)

Gandalph_5
(scaffolding protein)

Gandalph_6
(major capsid protein)

Gandalph_7
(head-to-tail adaptor)

Gandalph_8
(head-to-tail stopper)

Gandalph_9

Gandalph_10
(tail terminator)

Gandalph_11
(major tail protein)

Gandalph_12
(tail assembly chaperone)

Gandalph_13
(tail assembly chaperone)

Gandalph_14
(tape measure protein)

Gandalph_15
(minor tail protein)

Gandalph_16
(minor tail protein)

Gandalph_17
(minor tail protein)

Gandalph_18
(minor tail protein)

Gandalph_19
(minor tail protein, D-ala-D-ala carboxypeptidase)

Gandalph_20
(minor tail protein)

Gandalph_21

Gandalph_22

Gandalph_23

Gandalph_24

Gandalph_25
(helix-turn-helix DNA binding domain)

Gandalph_26

Gandalph_27

Gandalph_28

Gandalph_29

Gandalph_30

Gandalph_31
(lysin A)

Gandalph_32
(lysin B)

Gandalph_33
(holin)

Gandalph_34

Gandalph_35

Gandalph_36
(DnaQ-like (DNA polymerase III subunit))

Gandalph_37

Gandalph_38

Gandalph_39

Gandalph_40

Gandalph_41

Gandalph_42
(tyrosine integrase)

Gandalph_43

Gandalph_44

Gandalph_45
(immunity repressor)

Gandalph_46
(Cro (control of repressor's operator))

Gandalph_47
(antirepressor)

Gandalph_48

Gandalph_49

Gandalph_50

Gandalph_51
(excise)

Gandalph_52

Gandalph_53

Gandalph_54

Gandalph_55

Gandalph_56

Gandalph_57

Gandalph_58

Gandalph_59

Gandalph_60
(WhiB family transcription factor)

Gandalph_61
(helix-turn-helix DNA binding domain)

Gandalph_62
(WhiB family transcription factor)

Gandalph_63
(helix-turn-helix DNA binding domain)

Gandalph_64

Gandalph_65

Gandalph_66

Gandalph_67
(RecA-like DNA recombinase)

Gandalph_68
(HNH endonuclease)

Gandalph_69

Gandalph_70

Gandalph_71

Gandalph_72

Gandalph_73
(helix-turn-helix DNA binding domain)

Gandalph_74

Gandalph_75

Gandalph_76

Gandalph_77

Gandalph_78

Gandalph_79

Gandalph_80

Gandalph_81
(mycobacteriophage mobile element 1 (MPME 1))

Gandalph_82

Gandalph_83

Gandalph_84

Gandalph_85

Gandalph_86

Gandalph_87

Gandalph_88

Gandalph_89

Gandalph_90

Gandalph_91

Gandalph_92

Gandalph_93

Gandalph_94

Gandalph_95

Gandalph_96

Gandalph_97

Gandalph_98

Gandalph_99

Gandalph_100

Gandalph_101
(glycosyltransferase)

Gandalph_102

Gandalph_103
(glycosyltransferase)

Gandalph_104
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMT684586
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Designator 2011GONZgandalphEAC
Pitt Freezer Box# 24
Pitt Freezer Box Grid# 17
Available Files
Plaque PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload