| Detailed Information for Phage Radiance |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Jocelyn Seadler |
| Year Found | 2022 |
| Location Found | Bowling Green, KY United States |
| Finding Institution | Western Kentucky University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 37.00111 N, 86.43 W Map |
| Discovery Notes | Sample was collected directly below the surface of Barren River. May have contained sediment from nearby rocks. Sample was refrigerated for 48 hours. |
| Naming Notes | I chose this name for my phage because the plaques formed from the initial spot plate produced plaques with halos around them, and the halos reminded me of how the Sun's rays can appear. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 20, 2023 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 1713 |
| Genome length (bp) | 57325 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGACGGCGC |
| GC Content | 61.2% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | F |
| Subcluster | F1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Western Kentucky University |
| Annotation Status | In GenBank |
| Plaque Notes | At lower dilutions, a few 1mm clear plaques and numerous 0.5mm turbid plaques are formed. At higher dilutions, only 1mm clear plaques are formed. This suggests that mutants are present in the phage population. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | OR253910 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
2.95 x 10¹¹ |
| Pitt Freezer Box# |
157 |
| Pitt Freezer Box Grid# |
G12 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
