Mycobacterium phage Radiance
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Detailed Information for Phage Radiance
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByJocelyn Seadler
Year Found2022
Location FoundBowling Green, KY United States
Finding InstitutionWestern Kentucky University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature30°C
GPS Coordinates37.00111 N, 86.43 W Map
Discovery NotesSample was collected directly below the surface of Barren River. May have contained sediment from nearby rocks. Sample was refrigerated for 48 hours.
Naming NotesI chose this name for my phage because the plaques formed from the initial spot plate produced plaques with halos around them, and the halos reminded me of how the Sun's rays can appear.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 20, 2023
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1713
Genome length (bp)57325
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGACGGCGC
GC Content61.2%
Fasta file available?Yes: Download fasta file
Characterization
ClusterF
SubclusterF1
Cluster Life CycleTemperate
Other Cluster Members

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Blarby (None)

MulchSalad (None)

Akhila (F1)

Alexphander (F1)

AlpineSix (F1)

ArcusAngelus (F1)

Ardmore (F1)

Aubs (F1)

Awesomesauce (F1)

Batiatus (F1)

Beakin (F1)

BigPhil (F1)

Bipolar (F1)

Blexus (F1)

BlueCrab (F1)

BobaPhett (F1)

Bobi (F1)

BodEinwohner17 (F1)

Boomer (F1)

Brocalys (F1)

Bubbles123 (F1)

Burwell21 (F1)

BuzzLyseyear (F1)

ByChance (F1)

Byougenkin (F1)

Cabrinians (F1)

CaptainTrips (F1)

Cerasum (F1)

Che8 (F1)

Chevrolet (F1)

ChickenDinner (F1)

Chuckly (F1)

Clifton (F1)

Coco12 (F1)

Cornucopia (F1)

Crumble (F1)

DaddyRickover (F1)

Daenerys (F1)

Dante (F1)

DaWorst (F1)

DeadP (F1)

Deb65 (F1)

DillTech15 (F1)

DirtMcgirt (F1)

DLane (F1)

DocMcStuffins (F1)

Donkeykong (F1)

Doomphist (F1)

Dorothy (F1)

DosHalletts (F1)

DotProduct (F1)

Doug (F1)

Drago (F1)

DRBy19 (F1)

Eish (F1)

EleanorGeorge (F1)

Emma (F1)

Empress (F1)

Enby (F1)

Eradicator (F1)

Estave1 (F1)

Fancypants (F1)

Filuzino (F1)

Firehouse51 (F1)

Flathead (F1)

Florinda (F1)

Frankie (F1)

FreddyB (F1)

Fruitloop (F1)

Galactic (F1)

Gandalph (F1)

Geralt (F1)

GigiOuiOui (F1)

Girafales (F1)

Girr (F1)

Gorge (F1)

GUmbie (F1)

Guppsters (F1)

Hades (F1)

Hamulus (F1)

Harley (F1)

Hegedechwinu (F1)

Hlubikazi (F1)

Ibhubesi (F1)

IbOuu (F1)

Inventum (F1)

IrishSherpFalk (F1)

JalFarm20 (F1)

James (F1)

Jarcob (F1)

Jinglebell (F1)

Job42 (F1)

JoeyJr (F1)

Juice456 (F1)

Juniper1 (F1)

Karhdo (F1)

Kenuha5 (F1)

Kersh (F1)

Kimberlium (F1)

KingMidas (F1)

Kingsley (F1)

Koella (F1)

Krakatau (F1)

KristaRAM (F1)

LastJedi (F1)

Latretium (F1)

Leozinho (F1)

Leperchaun (F1)

LilMoolah (F1)

LittleShirley (F1)

Lizziana (F1)

Llama (F1)

Llij (F1)

Lorde (F1)

LunaBlu (F1)

Madiba (F1)

MadMen (F1)

Mahavrat (F1)

Mandlovu (F1)

Mantra (F1)

Maravista (F1)

Marker (F1)

Martik (F1)

Mattes (F1)

Melissauren88 (F1)

MilanaBonita (F1)

MilleniumForce (F1)

MinionDave (F1)

Minnie (F1)

Misha28 (F1)

MisterCuddles (F1)

Modragons (F1)

Moonbeam (F1)

Mova (F1)

Mozy (F1)

Ms6 (F1)

Mutaforma13 (F1)

Nimbo (F1)

Nitzel (F1)

Nivrat (F1)

NormanBulbieJr (F1)

Ochi17 (F1)

OfUltron (F1)

Ogopogo (F1)

Oksu (F1)

OldBen (F1)

OlympiaSaint (F1)

Ovechkin (F1)

OwlsT2W (F1)

Pacc40 (F1)

Phalconet (F1)

Phanphagia (F1)

PHappiness (F1)

Phasih (F1)

Phatniss (F1)

PhesterPhotato (F1)

Philus (F1)

Piper2020 (F1)

Pippy (F1)

Plumbus (F1)

PMC (F1)

Poenanya (F1)

Polka14 (F1)

Pollywog (F1)

PopTart (F1)

Priscilla (F1)

QuickMath (F1)

Quico (F1)

Radiance (F1)

Ramsey (F1)

RedBird (F1)

Rialto (F1)

Rita (F1)

RitaG (F1)

Rockne (F1)

RockyHorror (F1)

Royals2015 (F1)

Ruby (F1)

Saal (F1)

Sabbb (F1)

Sandalphon (F1)

Sarma624 (F1)

Sassafras (F1)

SassyB (F1)

Scottish (F1)

Seabastian (F1)

Seagreen (F1)

SG4 (F1)

Shauna1 (F1)

ShiLan (F1)

ShowerHandel (F1)

ShroomBoi (F1)

SimranZ1 (F1)

SiSi (F1)

Sparkdehlily (F1)

Spartacus (F1)

Spikelee (F1)

Spoonbill (F1)

StAnnes (F1)

Stap (F1)

Starcevich (F1)

Strokeseat (F1)

SuperGrey (F1)

SwagPigglett (F1)

Taj (F1)

TDanisky (F1)

TootsiePop (F1)

Tootsieroll (F1)

Totinger (F1)

Tweety (F1)

UncleRicky (F1)

Velveteen (F1)

Veteran (F1)

VRedHorse (F1)

Wachhund (F1)

Wee (F1)

Whatsapiecost (F1)

Whouxphf (F1)

WillSterrel (F1)

XFactor (F1)

Yorick (F1)

Zerg (F1)

Zizzle (F1)

Avani (F2)

Che9d (F2)

Demsculpinboyz (F2)

Jabbawokkie (F2)

Soul22 (F2)

Yoshi (F2)

Zapner (F2)

Squirty (F3)

LunaStella (F4)

Renaud18 (F4)

TChen (F4)

ThetaBob (F4)

Cornie (F5)

Annotating InstitutionWestern Kentucky University
Annotation StatusIn GenBank
Plaque NotesAt lower dilutions, a few 1mm clear plaques and numerous 0.5mm turbid plaques are formed. At higher dilutions, only 1mm clear plaques are formed. This suggests that mutants are present in the phage population.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Radiance_1
(Terminase small subunit)

Radiance_2
(Terminase large subunit)

Radiance_3
(portal protein)

Radiance_4
(capsid maturation protease)

Radiance_5
(scaffolding protein)

Radiance_6
(major capsid protein)

Radiance_7
(head-to-tail adaptor)

Radiance_8
(head-to-tail stopper)

Radiance_9

Radiance_10
(tail terminator)

Radiance_11
(major tail protein)

Radiance_12
(tail assembly chaperone)

Radiance_13
(tail assembly chaperone)

Radiance_14
(tape measure protein)

Radiance_15
(minor tail protein)

Radiance_16
(minor tail protein)

Radiance_17
(minor tail protein)

Radiance_18
(minor tail protein)

Radiance_19
(minor tail protein)

Radiance_20
(minor tail protein)

Radiance_21

Radiance_22

Radiance_23
(minor tail protein)

Radiance_24

Radiance_25
(helix-turn-helix DNA binding domain protein)

Radiance_26

Radiance_27

Radiance_28

Radiance_29

Radiance_30
(lysin A)

Radiance_31
(lysin B)

Radiance_32
(holin)

Radiance_33

Radiance_34

Radiance_35
(DnaQ-like DNA polymerase III subunit)

Radiance_36

Radiance_37

Radiance_38

Radiance_39

Radiance_40

Radiance_41
(helix-turn-helix DNA binding domain protein)

Radiance_42
(tyrosine integrase)

Radiance_43

Radiance_44
(immunity repressor)

Radiance_45
(Cro protein)

Radiance_46

Radiance_47
(ParB-like dsDNA partitioning protein)

Radiance_48

Radiance_49

Radiance_50
(excise)

Radiance_51

Radiance_52

Radiance_53

Radiance_54

Radiance_55

Radiance_56
(WhiB family transcription factor)

Radiance_57

Radiance_58
(WhiB family transcription factor)

Radiance_59
(helix-turn-helix DNA binding domain protein)

Radiance_60

Radiance_61

Radiance_62

Radiance_63
(SSB protein)

Radiance_64
(HNH endonuclease)

Radiance_65
(helicase)

Radiance_66

Radiance_67

Radiance_68

Radiance_69

Radiance_70

Radiance_71

Radiance_72
(RepA-like replication initiator)

Radiance_73
(DnaC-like helicase loader)

Radiance_74

Radiance_75

Radiance_76

Radiance_77

Radiance_78

Radiance_79

Radiance_80

Radiance_81

Radiance_82

Radiance_83

Radiance_84

Radiance_85

Radiance_86

Radiance_87

Radiance_88

Radiance_89

Radiance_90

Radiance_91
(G-I-Y Y-I-G endonuclease)

Radiance_92

Radiance_93

Radiance_94

Radiance_95

Radiance_96

Radiance_97

Radiance_98

Radiance_99
(glycosyltransferase)

Radiance_100

Radiance_101

Radiance_102
(glycosyltransferase)

Radiance_103
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionOR253910
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 2.95 x 10¹¹
Pitt Freezer Box# 157
Pitt Freezer Box Grid# G12
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload