Mycobacterium phage Cornie
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Detailed Information for Phage Cornie
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByJustin Lugo
Year Found2016
Location FoundFayetteville, NC USA
Finding InstitutionFayetteville State University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature37°C
GPS Coordinates35.01814 N, 78.895187 W Map
Discovery NotesWeather was an overcast with cool temps in the low 70s. It had rain the previous evening. The location of the sample was the public vehicle area(PVA) water run off a Waffle House parking lot. The dumpster was at the other side of the PVA. There was several debris, ants, and litter. The top layer was moved and the sample was then taken.
Naming NotesThe name I chose was a childhood nickname. I use to make lame jokes and my friends called me out on it all the time. For several years my friends would only refer to me as Cornie.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedDec 9, 2016
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1200
Genome length (bp)56391
Character of genome ends3' Sticky Overhang
Overhang Length9 bases
Overhang SequenceCCCGGCCCA
GC Content61.4%
Fasta file available?Yes: Download fasta file
Characterization
ClusterF
SubclusterF5
Cluster Life CycleTemperate
Lysogeny NotesThe integrase gene of this phage is missing the N-terminus and is fused to the DNA pol gene upstream of it. We tried to generate lysogens and were unsuccessful. Therefore, we conclude that this integrase is non-functional.
Other Cluster Members

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Soul22 (None)

Akhila (F1)

Alexphander (F1)

ArcusAngelus (F1)

Ardmore (F1)

Awesomesauce (F1)

Batiatus (F1)

BigPhil (F1)

Bipolar (F1)

Blexus (F1)

BobaPhett (F1)

Bobi (F1)

BodEinwohner17 (F1)

Boomer (F1)

Brocalys (F1)

Bubbles123 (F1)

Burwell21 (F1)

BuzzLyseyear (F1)

ByChance (F1)

Byougenkin (F1)

Cabrinians (F1)

CaptainTrips (F1)

Cerasum (F1)

Che8 (F1)

Chuckly (F1)

Clifton (F1)

Coco12 (F1)

Cornucopia (F1)

Daenerys (F1)

Dante (F1)

DaWorst (F1)

DeadP (F1)

DillTech15 (F1)

DirtMcgirt (F1)

DLane (F1)

Donkeykong (F1)

Doomphist (F1)

Dorothy (F1)

DotProduct (F1)

Doug (F1)

Drago (F1)

DRBy19 (F1)

Eish (F1)

EleanorGeorge (F1)

Emma (F1)

Empress (F1)

Enby (F1)

Estave1 (F1)

Fancypants (F1)

Filuzino (F1)

Firehouse51 (F1)

Flathead (F1)

Florinda (F1)

Frankie (F1)

Fruitloop (F1)

Galactic (F1)

Gandalph (F1)

Geralt (F1)

Girafales (F1)

Girr (F1)

Gorge (F1)

GUmbie (F1)

Hades (F1)

Hamulus (F1)

Harley (F1)

Hegedechwinu (F1)

Hlubikazi (F1)

Ibhubesi (F1)

Inventum (F1)

IrishSherpFalk (F1)

James (F1)

Job42 (F1)

JoeyJr (F1)

Juice456 (F1)

Kenuha5 (F1)

Kersh (F1)

Kimberlium (F1)

KingMidas (F1)

Kingsley (F1)

Koella (F1)

Krakatau (F1)

KristaRAM (F1)

LastJedi (F1)

Latretium (F1)

LilMoolah (F1)

Lizziana (F1)

Llama (F1)

Llij (F1)

Lorde (F1)

Mahavrat (F1)

Mandlovu (F1)

Mantra (F1)

Martik (F1)

Mattes (F1)

Melissauren88 (F1)

MilanaBonita (F1)

MilleniumForce (F1)

MinionDave (F1)

Minnie (F1)

Misha28 (F1)

MisterCuddles (F1)

Modragons (F1)

Moonbeam (F1)

Mova (F1)

Mozy (F1)

Mutaforma13 (F1)

Nimbo (F1)

Nitzel (F1)

Nivrat (F1)

NormanBulbieJr (F1)

Ochi17 (F1)

OfUltron (F1)

Ogopogo (F1)

OldBen (F1)

OlympiaSaint (F1)

Ovechkin (F1)

OwlsT2W (F1)

Pacc40 (F1)

Phanphagia (F1)

PHappiness (F1)

Phasih (F1)

Phatniss (F1)

Piper2020 (F1)

Pippy (F1)

Plumbus (F1)

PMC (F1)

Poenanya (F1)

Polka14 (F1)

Pollywog (F1)

PopTart (F1)

Priscilla (F1)

QuickMath (F1)

Quico (F1)

Ramsey (F1)

RitaG (F1)

RockyHorror (F1)

Royals2015 (F1)

Ruby (F1)

Saal (F1)

Sandalphon (F1)

Sassafras (F1)

SassyB (F1)

Scottish (F1)

Seabastian (F1)

Seagreen (F1)

SG4 (F1)

Shauna1 (F1)

ShiLan (F1)

SimranZ1 (F1)

SiSi (F1)

Sparkdehlily (F1)

Spartacus (F1)

Spikelee (F1)

Spoonbill (F1)

StAnnes (F1)

Strokeseat (F1)

SuperGrey (F1)

SwagPigglett (F1)

Taj (F1)

TDanisky (F1)

TootsiePop (F1)

Tweety (F1)

UncleRicky (F1)

Velveteen (F1)

Veteran (F1)

Wachhund (F1)

Wee (F1)

Whouxphf (F1)

WillSterrel (F1)

XFactor (F1)

Zerg (F1)

Avani (F2)

Che9d (F2)

Demsculpinboyz (F2)

Jabbawokkie (F2)

Yoshi (F2)

Zapner (F2)

Squirty (F3)

Renaud18 (F4)

TChen (F4)

ThetaBob (F4)

Cornie (F5)

Annotating InstitutionRockland Community College
Annotation StatusIn GenBank
Plaque NotesSmall (1mm) circular plaques with the edges appearing to defined. Sometimes displays short comet tails.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Cornie_1

Cornie_2
(terminase large subunit)

Cornie_3
(portal protein)

Cornie_4
(scaffolding protein)

Cornie_5
(capsid decoration protein)

Cornie_6
(major capsid protein)

Cornie_7

Cornie_8
(head-to-tail adaptor)

Cornie_9

Cornie_10

Cornie_11

Cornie_12
(major tail protein)

Cornie_13
(tail assembly chaperone)

Cornie_14
(tail assembly chaperone)

Cornie_15
(tape measure protein)

Cornie_16
(minor tail protein)

Cornie_17
(minor tail protein)

Cornie_18
(minor tail protein)

Cornie_19
(minor tail protein)

Cornie_20
(minor tail protein)

Cornie_21
(minor tail protein)

Cornie_22
(minor tail protein)

Cornie_23

Cornie_24
(G-I-Y Y-I-G endonuclease)

Cornie_25
(DNA binding protein)

Cornie_26

Cornie_27

Cornie_28

Cornie_29

Cornie_30
(lysin A)

Cornie_31
(lysin B)

Cornie_32
(holin)

Cornie_33

Cornie_34

Cornie_35
(tyrosine integrase)

Cornie_36

Cornie_37
(immunity repressor)

Cornie_38
(Cro protein)

Cornie_39
(antirepressor)

Cornie_40

Cornie_41

Cornie_42

Cornie_43
(helix-turn-helix DNA binding protein)

Cornie_44

Cornie_45

Cornie_46

Cornie_47

Cornie_48

Cornie_49

Cornie_50

Cornie_51

Cornie_52
(WhiB family transcription factor)

Cornie_53
(helix-turn-helix DNA binding domain protein)

Cornie_54
(helix-turn-helix DNA binding domain protein)

Cornie_55
(WhiB family transcription factor)

Cornie_56
(helix-turn-helix DNA binding domain protein)

Cornie_57

Cornie_58

Cornie_59

Cornie_60

Cornie_61

Cornie_62
(RecA-like DNA recombinase)

Cornie_63
(HNH endonuclease)

Cornie_64
(DNA methylase)

Cornie_65

Cornie_66

Cornie_67

Cornie_68

Cornie_69
(G-I-Y Y-I-G endonuclease)

Cornie_70

Cornie_71

Cornie_72

Cornie_73

Cornie_74

Cornie_75

Cornie_76

Cornie_77

Cornie_78

Cornie_79

Cornie_80

Cornie_81

Cornie_82

Cornie_83

Cornie_84

Cornie_85

Cornie_86

Cornie_87

Cornie_88

Cornie_89

Cornie_90
(helix-turn-helix DNA binding domain protein)

Cornie_91
(G-I-Y Y-I-G endonuclease)

Cornie_92

Cornie_93

Cornie_94

Cornie_95

Cornie_96

Cornie_97

Cornie_98
(O-methyltransferase)

Cornie_99
(glycosyltransferase)

Cornie_100
(galactosyltransferase)

Cornie_101
(HNH endonuclease)

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMN908688
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 1.3 x 10^-10
Date of SEA Lysate Titering Nov 2, 2016
Pitt Freezer Box# 66
Pitt Freezer Box Grid# A9
Available Files
Plaque PictureDownload
GenBank File for PhameratorDownload