Mycobacterium phage Awesomesauce
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Detailed Information for Phage Awesomesauce
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByCharlotte Merzbacher
Year Found2015
Location FoundProvidence, RI USA
Finding InstitutionBrown University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates41.824593 N, 71.40338 W Map
Discovery NotesThe phage was isolated from damp soil found under the bushes on the north side of Everett-Poland House in Keeney Quad. It had not rained for several days, so this spot was ideal because it still held residual moisture from the last rain. The temperature was approximately 85 degrees Fahrenheit and the depth of the sample dug was 2 inches deep.
Naming NotesAwesomesauce is truly an awesome phage, and it is bit saucy. I wanted my phage to feel empowered by its epic name.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 26, 2016
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage2640
Genome length (bp)57054
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCCGAAGGCAT
GC Content62.9%
Fasta file available?Yes: Download fasta file
Characterization
ClusterF
SubclusterF1
Cluster Life CycleTemperate
Other Cluster Members

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Soul22 (None)

Akhila (F1)

Alexphander (F1)

ArcusAngelus (F1)

Ardmore (F1)

Awesomesauce (F1)

Batiatus (F1)

BigPhil (F1)

Bipolar (F1)

Blexus (F1)

BobaPhett (F1)

Bobi (F1)

BodEinwohner17 (F1)

Boomer (F1)

Brocalys (F1)

Bubbles123 (F1)

Burwell21 (F1)

BuzzLyseyear (F1)

ByChance (F1)

Byougenkin (F1)

Cabrinians (F1)

CaptainTrips (F1)

Cerasum (F1)

Che8 (F1)

Chuckly (F1)

Clifton (F1)

Coco12 (F1)

Cornucopia (F1)

Daenerys (F1)

Dante (F1)

DaWorst (F1)

DeadP (F1)

DillTech15 (F1)

DirtMcgirt (F1)

DLane (F1)

Donkeykong (F1)

Doomphist (F1)

Dorothy (F1)

DotProduct (F1)

Doug (F1)

Drago (F1)

DRBy19 (F1)

Eish (F1)

EleanorGeorge (F1)

Emma (F1)

Empress (F1)

Enby (F1)

Eradicator (F1)

Estave1 (F1)

Fancypants (F1)

Filuzino (F1)

Firehouse51 (F1)

Flathead (F1)

Florinda (F1)

Frankie (F1)

Fruitloop (F1)

Galactic (F1)

Gandalph (F1)

Geralt (F1)

Girafales (F1)

Girr (F1)

Gorge (F1)

GUmbie (F1)

Hades (F1)

Hamulus (F1)

Harley (F1)

Hegedechwinu (F1)

Hlubikazi (F1)

Ibhubesi (F1)

Inventum (F1)

IrishSherpFalk (F1)

James (F1)

Job42 (F1)

JoeyJr (F1)

Juice456 (F1)

Kenuha5 (F1)

Kersh (F1)

Kimberlium (F1)

KingMidas (F1)

Kingsley (F1)

Koella (F1)

Krakatau (F1)

KristaRAM (F1)

LastJedi (F1)

Latretium (F1)

LilMoolah (F1)

Lizziana (F1)

Llama (F1)

Llij (F1)

Lorde (F1)

Mahavrat (F1)

Mandlovu (F1)

Mantra (F1)

Martik (F1)

Mattes (F1)

Melissauren88 (F1)

MilanaBonita (F1)

MilleniumForce (F1)

MinionDave (F1)

Minnie (F1)

Misha28 (F1)

MisterCuddles (F1)

Modragons (F1)

Moonbeam (F1)

Mova (F1)

Mozy (F1)

Mutaforma13 (F1)

Nimbo (F1)

Nitzel (F1)

Nivrat (F1)

NormanBulbieJr (F1)

Ochi17 (F1)

OfUltron (F1)

Ogopogo (F1)

OldBen (F1)

OlympiaSaint (F1)

Ovechkin (F1)

OwlsT2W (F1)

Pacc40 (F1)

Phanphagia (F1)

PHappiness (F1)

Phasih (F1)

Phatniss (F1)

Piper2020 (F1)

Pippy (F1)

Plumbus (F1)

PMC (F1)

Poenanya (F1)

Polka14 (F1)

Pollywog (F1)

PopTart (F1)

Priscilla (F1)

QuickMath (F1)

Quico (F1)

Ramsey (F1)

RitaG (F1)

RockyHorror (F1)

Royals2015 (F1)

Ruby (F1)

Saal (F1)

Sandalphon (F1)

Sassafras (F1)

SassyB (F1)

Scottish (F1)

Seabastian (F1)

Seagreen (F1)

SG4 (F1)

Shauna1 (F1)

ShiLan (F1)

SimranZ1 (F1)

SiSi (F1)

Sparkdehlily (F1)

Spartacus (F1)

Spikelee (F1)

Spoonbill (F1)

StAnnes (F1)

Strokeseat (F1)

SuperGrey (F1)

SwagPigglett (F1)

Taj (F1)

TDanisky (F1)

TootsiePop (F1)

Tweety (F1)

UncleRicky (F1)

Velveteen (F1)

Veteran (F1)

Wachhund (F1)

Wee (F1)

Whouxphf (F1)

WillSterrel (F1)

XFactor (F1)

Zerg (F1)

Avani (F2)

Che9d (F2)

Demsculpinboyz (F2)

Jabbawokkie (F2)

Yoshi (F2)

Zapner (F2)

Squirty (F3)

Renaud18 (F4)

TChen (F4)

ThetaBob (F4)

Cornie (F5)

Annotating InstitutionCollege of Charleston
Annotation StatusIn GenBank
Plaque NotesPlaques are generally 1-2 mm in diameter (depending on incubation period) and very clear; the phage is most likely lytic.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Awesomesauce_1
(HNH endonuclease)

Awesomesauce_2
(terminase small subunit)

Awesomesauce_3
(terminase large subunit)

Awesomesauce_4
(portal protein)

Awesomesauce_5
(capsid maturation protease)

Awesomesauce_6
(scaffolding protein)

Awesomesauce_7
(major capsid protein)

Awesomesauce_8
(head-to-tail adaptor)

Awesomesauce_9
(head-to-tail stopper)

Awesomesauce_10

Awesomesauce_11
(tail terminator)

Awesomesauce_12
(major tail protein)

Awesomesauce_13
(tail assembly chaperone)

Awesomesauce_14
(tail assembly chaperone)

Awesomesauce_15
(tape measure protein)

Awesomesauce_16
(minor tail protein)

Awesomesauce_17
(minor tail protein)

Awesomesauce_18

Awesomesauce_19
(minor tail protein)

Awesomesauce_20
(minor tail protein)

Awesomesauce_21

Awesomesauce_22

Awesomesauce_23

Awesomesauce_24

Awesomesauce_25

Awesomesauce_26
(helix-turn-helix DNA binding domain protein)

Awesomesauce_27

Awesomesauce_28

Awesomesauce_29

Awesomesauce_30

Awesomesauce_31
(lysin A)

Awesomesauce_32
(lysin B)

Awesomesauce_33

Awesomesauce_34

Awesomesauce_35

Awesomesauce_36
(DNA polymerase III subunit)

Awesomesauce_37

Awesomesauce_38

Awesomesauce_39

Awesomesauce_41
(tyrosine integrase)

Awesomesauce_42

Awesomesauce_43

Awesomesauce_44
(immunity repressor)

Awesomesauce_45
(Cro protein)

Awesomesauce_46
(antirepressor)

Awesomesauce_47

Awesomesauce_48
(excise)

Awesomesauce_49

Awesomesauce_50

Awesomesauce_51

Awesomesauce_52

Awesomesauce_53

Awesomesauce_54

Awesomesauce_55

Awesomesauce_56

Awesomesauce_57

Awesomesauce_58

Awesomesauce_59
(WhiB family transcription factor)

Awesomesauce_60

Awesomesauce_61

Awesomesauce_62
(MPME1 protein)

Awesomesauce_63

Awesomesauce_64

Awesomesauce_65

Awesomesauce_66
(Cas4 family exonuclease)

Awesomesauce_67

Awesomesauce_68

Awesomesauce_69

Awesomesauce_70
(RusA-like resolvase)

Awesomesauce_71

Awesomesauce_72

Awesomesauce_73
(DNA methylase)

Awesomesauce_74
(HNH endonuclease)

Awesomesauce_75

Awesomesauce_76
(DNA methylase)

Awesomesauce_77

Awesomesauce_78

Awesomesauce_79

Awesomesauce_80

Awesomesauce_81

Awesomesauce_82

Awesomesauce_83

Awesomesauce_84

Awesomesauce_85
(helix-turn-helix DNA binding domain protein)

Awesomesauce_86
(DnaC-like helicase loader)

Awesomesauce_87
(helix-turn-helix DNA binding domain protein)

Awesomesauce_88

Awesomesauce_89

Awesomesauce_90

Awesomesauce_91

Awesomesauce_92

Awesomesauce_93
(glycosyltransferase)

Awesomesauce_94

Awesomesauce_95
(glycosyltransferase)

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMT553347
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
SEA Lysate Titer 7.2x10^10
Date of SEA Lysate Titering Oct 23, 2015
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload