Mycobacterium phage Pollywog
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Detailed Information for Phage Pollywog
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByGrant Hughes
Year Found2017
Location FoundManhattan , KS United States
Finding InstitutionKansas State University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature21°C
GPS Coordinates39.192467 N, 96.58427 W Map
Discovery Notesfound in September of 2017, roughly 4 inches deep in the dirt. There was a layer of mulch over the top of this dirt. was located right off of a main walking route through a college campus. The temperature that day was 65 to 70 degrees Fahrenheit. The sample was taken at 9:30 am.
Naming Notesin Stranger Things 2 the baby Demogorgon is considered a pollywog. so yeah this is a baby Demogorgon virus user be cautious. Just kidding but that is where the name is from.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 12, 2018
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1658
Genome length (bp)58397
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCCGATGGCAT
GC Content61.3%
Fasta file available?Yes: Download fasta file
Characterization
ClusterF
SubclusterF1
Cluster Life CycleTemperate
Other Cluster Members

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AbbyNormal (None)

Grib (None)

Kishmeesh (None)

KitCat (None)

MightyMorph (None)

Puck (None)

Renaud18 (None)

Sargento (None)

WIVsmall1220 (None)

Alexphander (F1)

Anaxagorus (F1)

AndrewsK (F1)

Ant1Dodson (F1)

ArcusAngelus (F1)

Ardmore (F1)

Arienne (F1)

Asmart (F1)

Awesomesauce (F1)

BabytheFifth (F1)

Barson (F1)

Batiatus (F1)

BigPhil (F1)

Bipolar (F1)

Blexus (F1)

BobaPhett (F1)

Bobi (F1)

BodEinwohner17 (F1)

BoomCrackaPop (F1)

Boomer (F1)

Braemar (F1)

Brocalys (F1)

Bubbles123 (F1)

Burwell21 (F1)

BuzzLyseyear (F1)

ByChance (F1)

Byougenkin (F1)

Cabrinians (F1)

Cakepop (F1)

CaptainTrips (F1)

Cerasum (F1)

Che8 (F1)

Chhibber (F1)

Chikondi (F1)

Chuckly (F1)

Clifton (F1)

Clover (F1)

CoolGuy (F1)

Cornucopia (F1)

Daenerys (F1)

Danda (F1)

Dante (F1)

DaWorst (F1)

DCrust15 (F1)

DeadP (F1)

Desmond (F1)

DillTech15 (F1)

DirtMcgirt (F1)

DLane (F1)

Docouto (F1)

Doomphist (F1)

Dorothy (F1)

DotProduct (F1)

Doug (F1)

Drago (F1)

DRBy19 (F1)

Eish (F1)

EleanorGeorge (F1)

Emma (F1)

Empress (F1)

EmWilla (F1)

Enby (F1)

Estave1 (F1)

Estella (F1)

Fancypants (F1)

Filuzino (F1)

Firehouse51 (F1)

Flathead (F1)

Florinda (F1)

Frankie (F1)

Freddie4 (F1)

Fruitloop (F1)

FunOne (F1)

Galactic (F1)

Gardregs (F1)

Gendisarray (F1)

Genesis (F1)

Geralt (F1)

Girafales (F1)

Girr (F1)

Gorge (F1)

GreatPumpkin (F1)

GUmbie (F1)

Hades (F1)

Hamulus (F1)

Harley (F1)

Hegedechwinu (F1)

Ibhubesi (F1)

Inventum (F1)

IrishSherpFalk (F1)

James (F1)

Job42 (F1)

JoeyJr (F1)

Judylove (F1)

Juice456 (F1)

Kalikimaka (F1)

Katya (F1)

Kenuha5 (F1)

Kersh (F1)

Keyes (F1)

Kimberlium (F1)

KingMidas (F1)

Kingsley (F1)

Koella (F1)

Krakatau (F1)

KristaRAM (F1)

LastJedi (F1)

Latretium (F1)

LillyLatzon (F1)

LilMoolah (F1)

Lizziana (F1)

Llama (F1)

Llij (F1)

Lorde (F1)

Lysinator (F1)

MacDrage (F1)

Mahavrat (F1)

Mantra (F1)

Mattes (F1)

McDiddy (F1)

Melissauren88 (F1)

MerrillCO18 (F1)

Mille (F1)

MilleniumForce (F1)

MinionDave (F1)

Minnie (F1)

Misha28 (F1)

MisterCuddles (F1)

Modragons (F1)

Mozy (F1)

Ms6 (F1)

Mutaforma13 (F1)

Myosotis (F1)

Nemain (F1)

Nimbo (F1)

Nitzel (F1)

Nivrat (F1)

Noodle (F1)

NormanBulbieJr (F1)

Ochi17 (F1)

OfUltron (F1)

Ogopogo (F1)

OldBen (F1)

OlympiaSaint (F1)

Ovechkin (F1)

OwlsT2W (F1)

Pacc40 (F1)

Peanut21s (F1)

Phabio (F1)

PHappiness (F1)

Phasih (F1)

Phatniss (F1)

PhrankthaTank (F1)

Piper2020 (F1)

Pippy (F1)

Plackberry (F1)

Plumbus (F1)

PMC (F1)

Poenanya (F1)

Polka14 (F1)

Pollywog (F1)

PopTart (F1)

Priscilla (F1)

Prometheus (F1)

QuickMath (F1)

Quico (F1)

Ramsey (F1)

RitaG (F1)

Rocktrap (F1)

RockyHorror (F1)

Rombolo (F1)

Royals2015 (F1)

Ruby (F1)

S2Kari (F1)

S2TSquared (F1)

Saal (F1)

SaltyMike (F1)

Sandalphon (F1)

SassyB (F1)

Scottish (F1)

Seagreen (F1)

SG4 (F1)

Sharpshooter (F1)

Shauna1 (F1)

SherL (F1)

ShiLan (F1)

SimranZ1 (F1)

SiSi (F1)

Sparkdehlily (F1)

Spartacus (F1)

Spikelee (F1)

Spoonbill (F1)

StAnnes (F1)

Strokeseat (F1)

SuperGrey (F1)

SwagPigglett (F1)

Taj (F1)

Taus (F1)

TDanisky (F1)

TonaldTDrump (F1)

TootsiePop (F1)

Tweety (F1)

UncleRicky (F1)

Velveteen (F1)

Wachhund (F1)

Wee (F1)

WheatBerry18 (F1)

Whouxphf (F1)

WillSterrel (F1)

WIVsmall (F1)

XFactor (F1)

Zerg (F1)

Avani (F2)

Che9d (F2)

Demsculpinboyz (F2)

Jabbawokkie (F2)

Yoshi (F2)

Zapner (F2)

Squirty (F3)

TChen (F4)

ThetaBob (F4)

Cornie (F5)

Annotation StatusIn GenBank
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Pollywog_1
(HNH endonuclease)

Pollywog_2
(terminase small subunit)

Pollywog_3
(terminase large subunit)

Pollywog_4
(portal protein)

Pollywog_5
(minor capsid protein)

Pollywog_6
(scaffolding protein)

Pollywog_7
(major capsid protein)

Pollywog_8
(head-to-tail adaptor)

Pollywog_9
(head-to-tail stopper)

Pollywog_10

Pollywog_11
(tail terminator)

Pollywog_12
(major tail protein)

Pollywog_13
(tail assembly chaperone)

Pollywog_14
(tail assembly chaperone)

Pollywog_15
(tape measure protein)

Pollywog_16
(transposase)

Pollywog_17
(minor tail protein)

Pollywog_18
(minor tail protein)

Pollywog_19
(minor tail protein)

Pollywog_20
(minor tail protein)

Pollywog_21
(minor tail protein)

Pollywog_22

Pollywog_23

Pollywog_24

Pollywog_25
(DNA binding domain protein)

Pollywog_26

Pollywog_27

Pollywog_28

Pollywog_29

Pollywog_30
(lysin A)

Pollywog_31
(lysin B)

Pollywog_32
(holin)

Pollywog_33

Pollywog_34

Pollywog_35
(DNA polymerase III subunit)

Pollywog_36

Pollywog_37

Pollywog_38

Pollywog_39

Pollywog_40

Pollywog_41
(HTH DNA binding domain protein)

Pollywog_42
(integrase)

Pollywog_43

Pollywog_44
(immunity repressor)

Pollywog_45
(Cro protein)

Pollywog_46
(antirepressor)

Pollywog_47

Pollywog_48
(excise)

Pollywog_49

Pollywog_50

Pollywog_51

Pollywog_52

Pollywog_53

Pollywog_54

Pollywog_55

Pollywog_56

Pollywog_57

Pollywog_58
(WhiB family transcription factor)

Pollywog_59

Pollywog_60

Pollywog_61
(helix-turn-helix DNA binding protein)

Pollywog_62

Pollywog_63

Pollywog_64

Pollywog_65
(AAA-ATPase)

Pollywog_66
(HNH endonuclease)

Pollywog_67
(DNA methylase)

Pollywog_68

Pollywog_69

Pollywog_70

Pollywog_71
(PAPS reductase-like domain protein)

Pollywog_72

Pollywog_73

Pollywog_74

Pollywog_75

Pollywog_76

Pollywog_77

Pollywog_78

Pollywog_79

Pollywog_80

Pollywog_81

Pollywog_82

Pollywog_83

Pollywog_84

Pollywog_85
(NrdH-like glutaredoxin)

Pollywog_86

Pollywog_87

Pollywog_88

Pollywog_89

Pollywog_90

Pollywog_91

Pollywog_92

Pollywog_93

Pollywog_94

Pollywog_95
(G-I-Y Y-I-G endonuclease)

Pollywog_96

Pollywog_97

Pollywog_98

Pollywog_99

Pollywog_100

Pollywog_101

Pollywog_102

Pollywog_103
(glycosyltransferase)

Pollywog_104

Pollywog_105
(glycosyltransferase)

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK359343
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 49
Pitt Freezer Box Grid# A10
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
GenBank File for PhameratorDownload