Mycobacterium phage Pollywog
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Detailed Information for Phage Pollywog
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByGrant Hughes
Year Found2017
Location FoundManhattan , KS United States
Finding InstitutionKansas State University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature21°C
GPS Coordinates39.192467 N, 96.58427 W Map
Discovery Notesfound in September of 2017, roughly 4 inches deep in the dirt. There was a layer of mulch over the top of this dirt. was located right off of a main walking route through a college campus. The temperature that day was 65 to 70 degrees Fahrenheit. The sample was taken at 9:30 am.
Naming Notesin Stranger Things 2 the baby Demogorgon is considered a pollywog. so yeah this is a baby Demogorgon virus user be cautious. Just kidding but that is where the name is from.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedDec 8, 2018
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage396
Genome length (bp)73453
Character of genome endsDirect Terminal Repeat
Direct Terminal Repeat Length161 bp
GC Content59.0%
Fasta file available?Yes: Download fasta file
Characterization
ClusterCS
SubclusterCS3
Cluster Life CycleLytic
Other Cluster Members
Annotation StatusBeing Annotated (Expected completion by 5/15/2019)
Plaque NotesTiny pinholes (0.5-1mm) with a few large plaques (3mm) in the lawn.
Has been Phamerated?No
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 49
Pitt Freezer Box Grid# A10
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload