| Detailed Information for Phage CaptainTrips |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Emily Powell |
| Year Found | 2012 |
| Location Found | Woodville, AL USA |
| Finding Institution | Jacksonville State University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 34.573975 N, 86.221176 W Map |
| Discovery Notes | Sample was collected at Cathedral Caverns State Park, located in northern Marshall County, AL. The sample was taken from the top bank of Mystery River, which flows through the cave.
Sample collection depth: 2 cm
Collection date: September 1, 2012
Air temperature at collection: 15.6 degrees C.
Approximate moisture content: Saturated/extremely moist |
| Naming Notes | CaptainTrips is named in honor of the man-made superflu (influenza) virus featured in Stephen King's book "The Stand". Captain Trips is accidentally released from a military facility, and subsequently kills over 99% of the global population. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 10, 2013 |
| Sequencing Facility | Virginia Commonwealth University Nucleic Acids Research Facilities |
| Shotgun Sequencing Method | 454 Pyrosequencing |
| Approximate Shotgun Coverage | 320 |
| Genome length (bp) | 57328 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGAAGGCGC |
| GC Content | 61.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | F |
| Subcluster | F1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Morphotype | Siphoviridae |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KM652553 |
| Refseq Number | None yet |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info |
| Archiving status |
Archived |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
