Mycobacterium phage KingMidas
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Detailed Information for Phage KingMidas
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByZachary Jordan
Year Found2016
Location FoundProvidence, RI USA
Finding InstitutionBrown University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature23°C
GPS Coordinates40.27018 N, 97.126 W Map
Discovery NotesThis soil was collected from underneath a sprinkler head in Keeney Quad, at Brown University. It consisted mostly of decomposing leaf and plant matter, and was damp. Collected at night, when the soil was wettest.
Naming NotesKing Midas is a figure in Greek Mythology best known for his ability to turn objects or people to gold by touching them. As a result, he killed everything living that he touched, as does this phage with bacteria.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 3, 2017
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1672
Genome length (bp)57386
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGACGGCGC
GC Content61.4%
Fasta file available?Yes: Download fasta file
Characterization
ClusterF
SubclusterF1
Cluster Life CycleTemperate
Other Cluster Members

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Renaud18 (None)

Alexphander (F1)

ArcusAngelus (F1)

Ardmore (F1)

Awesomesauce (F1)

Batiatus (F1)

BigPhil (F1)

Bipolar (F1)

Blexus (F1)

BobaPhett (F1)

Bobi (F1)

BodEinwohner17 (F1)

Boomer (F1)

Brocalys (F1)

Bubbles123 (F1)

Burwell21 (F1)

BuzzLyseyear (F1)

ByChance (F1)

Byougenkin (F1)

Cabrinians (F1)

CaptainTrips (F1)

Cerasum (F1)

Che8 (F1)

Chuckly (F1)

Clifton (F1)

Cornucopia (F1)

Daenerys (F1)

Dante (F1)

DaWorst (F1)

DeadP (F1)

DillTech15 (F1)

DirtMcgirt (F1)

DLane (F1)

Donkeykong (F1)

Doomphist (F1)

Dorothy (F1)

DotProduct (F1)

Doug (F1)

Drago (F1)

DRBy19 (F1)

Eish (F1)

EleanorGeorge (F1)

Emma (F1)

Empress (F1)

Enby (F1)

Estave1 (F1)

Fancypants (F1)

Filuzino (F1)

Firehouse51 (F1)

Flathead (F1)

Florinda (F1)

Frankie (F1)

Fruitloop (F1)

Galactic (F1)

Geralt (F1)

Girafales (F1)

Girr (F1)

Gorge (F1)

GUmbie (F1)

Hades (F1)

Hamulus (F1)

Harley (F1)

Hegedechwinu (F1)

Ibhubesi (F1)

Inventum (F1)

IrishSherpFalk (F1)

James (F1)

Job42 (F1)

JoeyJr (F1)

Juice456 (F1)

Kenuha5 (F1)

Kersh (F1)

Kimberlium (F1)

KingMidas (F1)

Kingsley (F1)

Koella (F1)

Krakatau (F1)

KristaRAM (F1)

LastJedi (F1)

Latretium (F1)

LilMoolah (F1)

Lizziana (F1)

Llama (F1)

Llij (F1)

Lorde (F1)

Mahavrat (F1)

Mantra (F1)

Mattes (F1)

Melissauren88 (F1)

MilleniumForce (F1)

MinionDave (F1)

Minnie (F1)

Misha28 (F1)

MisterCuddles (F1)

Modragons (F1)

Mozy (F1)

Mutaforma13 (F1)

Nimbo (F1)

Nitzel (F1)

Nivrat (F1)

NormanBulbieJr (F1)

Ochi17 (F1)

OfUltron (F1)

Ogopogo (F1)

OldBen (F1)

OlympiaSaint (F1)

Ovechkin (F1)

OwlsT2W (F1)

Pacc40 (F1)

PHappiness (F1)

Phasih (F1)

Phatniss (F1)

Piper2020 (F1)

Pippy (F1)

Plumbus (F1)

PMC (F1)

Poenanya (F1)

Polka14 (F1)

Pollywog (F1)

PopTart (F1)

Priscilla (F1)

QuickMath (F1)

Quico (F1)

Ramsey (F1)

RitaG (F1)

RockyHorror (F1)

Royals2015 (F1)

Ruby (F1)

Saal (F1)

Sandalphon (F1)

SassyB (F1)

Scottish (F1)

Seagreen (F1)

SG4 (F1)

Shauna1 (F1)

ShiLan (F1)

SimranZ1 (F1)

SiSi (F1)

Sparkdehlily (F1)

Spartacus (F1)

Spikelee (F1)

Spoonbill (F1)

StAnnes (F1)

Strokeseat (F1)

SuperGrey (F1)

SwagPigglett (F1)

Taj (F1)

TDanisky (F1)

TootsiePop (F1)

Tweety (F1)

UncleRicky (F1)

Velveteen (F1)

Wachhund (F1)

Wee (F1)

Whouxphf (F1)

WillSterrel (F1)

XFactor (F1)

Zerg (F1)

Avani (F2)

Che9d (F2)

Demsculpinboyz (F2)

Jabbawokkie (F2)

Yoshi (F2)

Zapner (F2)

Squirty (F3)

TChen (F4)

ThetaBob (F4)

Cornie (F5)

Annotating InstitutionCollege of Charleston
Annotation StatusQC Complete
Plaque NotesPlaques are clear, indicating a lytic phage. Diameter range: 1.46 – 3.24 mm (mean = 2.41 mm plus or minus 0.1 mm SE). Fairly consistent size and circular shape, with randomized distribution throughout the agar.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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KingMidas_1
(terminase, small subunit)

KingMidas_2
(terminase, large subunit)

KingMidas_3
(portal protein)

KingMidas_4
(capsid maturation protease)

KingMidas_5
(scaffolding protein)

KingMidas_6
(major capsid protein)

KingMidas_7
(head-to-tail adaptor)

KingMidas_8
(head-to-tail stopper)

KingMidas_9

KingMidas_10
(tail terminator)

KingMidas_11
(major tail protein)

KingMidas_12
(tail assembly chaperone)

KingMidas_13
(tail assembly chaperone)

KingMidas_14
(tape measure protein)

KingMidas_15
(transposase)

KingMidas_16
(minor tail protein)

KingMidas_17
(minor tail protein)

KingMidas_18

KingMidas_19
(minor tail protein)

KingMidas_20
(minor tail protein, D-ala-D-ala carboxypeptidase)

KingMidas_21

KingMidas_22

KingMidas_23

KingMidas_24

KingMidas_25

KingMidas_26
(helix-turn-helix DNA binding domain)

KingMidas_27

KingMidas_28

KingMidas_29
(membrane protein)

KingMidas_30

KingMidas_31
(lysin A)

KingMidas_32
(HNH endonuclease)

KingMidas_33
(lysin B)

KingMidas_34

KingMidas_35
(membrane protein)

KingMidas_36

KingMidas_37
(DnaQ-like (DNA polymerase III subunit))

KingMidas_38

KingMidas_39
(membrane protein)

KingMidas_40

KingMidas_41

KingMidas_42
(excise)

KingMidas_43

KingMidas_44
(tyrosine integrase)

KingMidas_45
(membrane protein)

KingMidas_46

KingMidas_47
(immunity repressor)

KingMidas_48
(Cro (control of repressor))

KingMidas_49

KingMidas_50
(helix-turn-helix DNA binding domain)

KingMidas_51

KingMidas_52

KingMidas_53

KingMidas_54

KingMidas_55
(membrane protein)

KingMidas_56
(WhiB family transcription factor)

KingMidas_57
(helix-turn-helix DNA binding domain)

KingMidas_58
(WhiB family transcription factor)

KingMidas_59
(helix-turn-helix DNA binding domain)

KingMidas_60

KingMidas_61

KingMidas_62
(Ku-like dsDNA break-binding protein)

KingMidas_63
(RecA-like DNA Recombinase)

KingMidas_64

KingMidas_65
(HNH endonuclease)

KingMidas_66

KingMidas_67

KingMidas_68
(exonuclease)

KingMidas_69
(DNA methylase)

KingMidas_70
(DNA methylase)

KingMidas_71

KingMidas_72

KingMidas_73
(DNA methylase)

KingMidas_74

KingMidas_75
(helix-turn-helix DNA binding domain)

KingMidas_76

KingMidas_77
(antirepressor)

KingMidas_78

KingMidas_79

KingMidas_80

KingMidas_81

KingMidas_82

KingMidas_83
(membrane protein)

KingMidas_84

KingMidas_85

KingMidas_86

KingMidas_87

KingMidas_88

KingMidas_89
(membrane protein)

KingMidas_90

KingMidas_91

KingMidas_92

KingMidas_93

KingMidas_94
(helix-turn-helix DNA binding domain)

KingMidas_95

KingMidas_96

KingMidas_97

KingMidas_98

KingMidas_99

KingMidas_100

KingMidas_101
(glycosyltransferase)

KingMidas_102

KingMidas_103

KingMidas_104
(glycosyltransferase)

KingMidas_105
(HNH endonuclease)

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
SEA Lysate Titer 2.21 x 10^11
Date of SEA Lysate Titering Oct 12, 2016
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload