Mycobacterium phage Eish
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Detailed Information for Phage Eish
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByIan Carleton
Year Found2018
Location FoundEmpangeni, South Africa
Finding InstitutionUniversity of Pittsburgh
ProgramPhage Hunters Integrating Research and Education
From enriched soil sample?Yes
Isolation Temperature37°C
GPS Coordinates28.8 S, 31.8 E Map
Discovery NotesDiscovered from a lysate of phage "Thonko" collected from Empangeni, South Africa by Nomusa Christina Mzimela. The lysate was plaque purified at the University of Pittsburgh, and this phage was discovered after plaque purification demonstrated two distinct plaque morphologies.
Naming NotesColloquial saying common in South African culture.
A South African word derived from Xhosa, now used in all languages within South Africa and some neighbouring countries. Eish is one of the reasons South Africa is so great, it can express anything from excitement to horror.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedApr 19, 2018
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage650
Genome length (bp)58150
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGACGGCGC
GC Content61.2%
Fasta file available?Yes: Download fasta file
Characterization
ClusterF
SubclusterF1
Cluster Life CycleTemperate
Other Cluster Members

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Renaud18 (None)

Alexphander (F1)

ArcusAngelus (F1)

Ardmore (F1)

Awesomesauce (F1)

Batiatus (F1)

BigPhil (F1)

Bipolar (F1)

Blexus (F1)

BobaPhett (F1)

Bobi (F1)

BodEinwohner17 (F1)

Boomer (F1)

Brocalys (F1)

Bubbles123 (F1)

Burwell21 (F1)

BuzzLyseyear (F1)

ByChance (F1)

Byougenkin (F1)

Cabrinians (F1)

CaptainTrips (F1)

Cerasum (F1)

Che8 (F1)

Chuckly (F1)

Clifton (F1)

Coco12 (F1)

Cornucopia (F1)

Daenerys (F1)

Dante (F1)

DaWorst (F1)

DeadP (F1)

DillTech15 (F1)

DirtMcgirt (F1)

DLane (F1)

Donkeykong (F1)

Doomphist (F1)

Dorothy (F1)

DotProduct (F1)

Doug (F1)

Drago (F1)

DRBy19 (F1)

Eish (F1)

EleanorGeorge (F1)

Emma (F1)

Empress (F1)

Enby (F1)

Estave1 (F1)

Fancypants (F1)

Filuzino (F1)

Firehouse51 (F1)

Flathead (F1)

Florinda (F1)

Frankie (F1)

Fruitloop (F1)

Galactic (F1)

Gandalph (F1)

Geralt (F1)

Girafales (F1)

Girr (F1)

Gorge (F1)

GUmbie (F1)

Hades (F1)

Hamulus (F1)

Harley (F1)

Hegedechwinu (F1)

Ibhubesi (F1)

Inventum (F1)

IrishSherpFalk (F1)

James (F1)

Job42 (F1)

JoeyJr (F1)

Juice456 (F1)

Kenuha5 (F1)

Kersh (F1)

Kimberlium (F1)

KingMidas (F1)

Kingsley (F1)

Koella (F1)

Krakatau (F1)

KristaRAM (F1)

LastJedi (F1)

Latretium (F1)

LilMoolah (F1)

Lizziana (F1)

Llama (F1)

Llij (F1)

Lorde (F1)

Mahavrat (F1)

Mantra (F1)

Mattes (F1)

Melissauren88 (F1)

MilleniumForce (F1)

MinionDave (F1)

Minnie (F1)

Misha28 (F1)

MisterCuddles (F1)

Modragons (F1)

Mozy (F1)

Mutaforma13 (F1)

Nimbo (F1)

Nitzel (F1)

Nivrat (F1)

NormanBulbieJr (F1)

Ochi17 (F1)

OfUltron (F1)

Ogopogo (F1)

OldBen (F1)

OlympiaSaint (F1)

Ovechkin (F1)

OwlsT2W (F1)

Pacc40 (F1)

Phanphagia (F1)

PHappiness (F1)

Phasih (F1)

Phatniss (F1)

Piper2020 (F1)

Pippy (F1)

Plumbus (F1)

PMC (F1)

Poenanya (F1)

Polka14 (F1)

Pollywog (F1)

PopTart (F1)

Priscilla (F1)

QuickMath (F1)

Quico (F1)

Ramsey (F1)

RitaG (F1)

RockyHorror (F1)

Royals2015 (F1)

Ruby (F1)

Saal (F1)

Sandalphon (F1)

SassyB (F1)

Scottish (F1)

Seabastian (F1)

Seagreen (F1)

SG4 (F1)

Shauna1 (F1)

ShiLan (F1)

SimranZ1 (F1)

SiSi (F1)

Sparkdehlily (F1)

Spartacus (F1)

Spikelee (F1)

Spoonbill (F1)

StAnnes (F1)

Strokeseat (F1)

SuperGrey (F1)

SwagPigglett (F1)

Taj (F1)

TDanisky (F1)

TootsiePop (F1)

Tweety (F1)

UncleRicky (F1)

Velveteen (F1)

Veteran (F1)

Wachhund (F1)

Wee (F1)

Whouxphf (F1)

WillSterrel (F1)

XFactor (F1)

Zerg (F1)

Avani (F2)

Che9d (F2)

Demsculpinboyz (F2)

Jabbawokkie (F2)

Yoshi (F2)

Zapner (F2)

Squirty (F3)

TChen (F4)

ThetaBob (F4)

Cornie (F5)

Annotating InstitutionUniversity of Pittsburgh
Annotation StatusQC Complete
Has been Phamerated?Yes
Gene List

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Eish_1
(terminase, small subunit)

Eish_2
(terminase, large subunit)

Eish_3
(portal protein)

Eish_4
(capsid maturation protease and MuF-like fusion protein)

Eish_5

Eish_6
(major capsid protein)

Eish_7
(head-to-tail adaptor)

Eish_8
(head-to-tail stopper)

Eish_9

Eish_10
(tail terminator)

Eish_11
(major tail protein)

Eish_12
(tail assembly chaperone)

Eish_13
(tail assembly chaperone)

Eish_14
(tape measure protein)

Eish_15
(minor tail protein)

Eish_16
(minor tail protein)

Eish_17
(minor tail protein)

Eish_18
(minor tail protein)

Eish_19
(minor tail protein, D-ala-D-ala)

Eish_20
(minor tail protein)

Eish_21

Eish_22

Eish_23

Eish_24

Eish_25

Eish_26

Eish_27

Eish_28

Eish_29

Eish_30
(lysin A)

Eish_31
(lysin B)

Eish_32
(holin)

Eish_33

Eish_34

Eish_35
(DnaQ-like (DNA polymerase III subunit))

Eish_36

Eish_37

Eish_38
(membrane protein)

Eish_39

Eish_40

Eish_41
(helix-turn-helix DNA binding domain)

Eish_42

Eish_43
(tyrosine integrase)

Eish_44

Eish_45

Eish_46
(immunity repressor)

Eish_47
(CRO (control of repressor s operator))

Eish_48
(antirepressor)

Eish_49

Eish_50
(helix-turn-helix DNA binding domain)

Eish_51

Eish_52

Eish_53

Eish_54

Eish_55

Eish_56

Eish_57

Eish_58

Eish_59

Eish_60

Eish_61

Eish_62
(WhiB family transcription factor)

Eish_63
(helix-turn-helix DNA binding domain)

Eish_64
(WhiB family transcription factor)

Eish_65
(helix-turn-helix DNA binding domain)

Eish_66

Eish_67

Eish_68
(HNH endonuclease)

Eish_69
(DNA helicase)

Eish_70

Eish_71

Eish_72

Eish_73
(PAPS reductase-like domain)

Eish_74

Eish_75

Eish_76

Eish_77

Eish_78

Eish_79

Eish_80

Eish_81

Eish_82

Eish_83

Eish_84

Eish_85

Eish_86
(mycobacteriophage mobile element 1)

Eish_87

Eish_88

Eish_89

Eish_90

Eish_91

Eish_92

Eish_93

Eish_94

Eish_95

Eish_96

Eish_97

Eish_98

Eish_99

Eish_100
(helix-turn-helix DNA binding domain, MerR-like)

Eish_101

Eish_102

Eish_103

Eish_104

Eish_105

Eish_106
(glycosyltransferase)

Eish_107

Eish_108
(glycosyltransferase)

Eish_109
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
Available Files
Final DNAMaster FileDownload
GenBank File for PhameratorDownload