Mycobacterium phage Shauna1
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Detailed Information for Phage Shauna1
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByMarshall Sheide
Year Found2010
Location FoundOrem, UT USA
Finding InstitutionBrigham Young University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates40.325986 N, 111.735480 W Map
Discovery NotesIt was right next to a strawberry patch on some moist ground. Temperature was about 70 degrees Fahrenheit, and it was found in some rich soil in a green house.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedMar 1, 2011
Genome length (bp)59315
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGTAGGCGC
GC Content61.7%
Fasta file available?Yes: Download fasta file
Characterization
ClusterF
SubclusterF1
Cluster Life CycleTemperate
Other Cluster Members

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AbbyNormal (None)

Grib (None)

Kishmeesh (None)

KitCat (None)

MightyMorph (None)

Puck (None)

Renaud18 (None)

WIVsmall1220 (None)

Alexphander (F1)

Anaxagorus (F1)

Ant1Dodson (F1)

ArcusAngelus (F1)

Ardmore (F1)

Arienne (F1)

Asmart (F1)

Awesomesauce (F1)

BabytheFifth (F1)

Barson (F1)

Batiatus (F1)

BigPhil (F1)

Bipolar (F1)

BobaPhett (F1)

Bobi (F1)

BodEinwohner17 (F1)

BoomCrackaPop (F1)

Boomer (F1)

Braemar (F1)

Brocalys (F1)

Bubbles123 (F1)

Burwell21 (F1)

BuzzLyseyear (F1)

ByChance (F1)

Byougenkin (F1)

Cabrinians (F1)

Cakepop (F1)

CaptainTrips (F1)

Cerasum (F1)

Che8 (F1)

Chhibber (F1)

Chikondi (F1)

Chuckly (F1)

Clifton (F1)

Clover (F1)

CoolGuy (F1)

Cornucopia (F1)

Daenerys (F1)

Danda (F1)

Dante (F1)

DaWorst (F1)

DCrust15 (F1)

DeadP (F1)

Desmond (F1)

DillTech15 (F1)

DirtMcgirt (F1)

DLane (F1)

Docouto (F1)

Dorothy (F1)

DotProduct (F1)

Drago (F1)

DRBy19 (F1)

Eish (F1)

EleanorGeorge (F1)

Emma (F1)

Empress (F1)

Enby (F1)

Estave1 (F1)

Estella (F1)

Fancypants (F1)

Filuzino (F1)

Firehouse51 (F1)

Flathead (F1)

Florinda (F1)

Frankie (F1)

Freddie4 (F1)

Fruitloop (F1)

FunOne (F1)

Galactic (F1)

Gardregs (F1)

Gendisarray (F1)

Genesis (F1)

Geralt (F1)

Girafales (F1)

Girr (F1)

Gorge (F1)

GreatPumpkin (F1)

GUmbie (F1)

Hades (F1)

Hamulus (F1)

Harley (F1)

Hegedechwinu (F1)

Ibhubesi (F1)

Inventum (F1)

IrishSherpFalk (F1)

James (F1)

Job42 (F1)

JoeyJr (F1)

Judylove (F1)

Kalikimaka (F1)

Katya (F1)

Kenuha5 (F1)

Kersh (F1)

Kimberlium (F1)

KingMidas (F1)

Kingsley (F1)

Koella (F1)

Krakatau (F1)

KristaRAM (F1)

Latretium (F1)

LillyLatzon (F1)

LilMoolah (F1)

Lizziana (F1)

Llama (F1)

Llij (F1)

Lorde (F1)

Lysinator (F1)

MacDrage (F1)

Mahavrat (F1)

Mantra (F1)

Mattes (F1)

McDiddy (F1)

Melissauren88 (F1)

MerrillCO18 (F1)

Mille (F1)

MilleniumForce (F1)

MinionDave (F1)

Minnie (F1)

Misha28 (F1)

MisterCuddles (F1)

Modragons (F1)

Mozy (F1)

Ms6 (F1)

Mutaforma13 (F1)

Myosotis (F1)

Nemain (F1)

Nimbo (F1)

Nitzel (F1)

Nivrat (F1)

Noodle (F1)

NormanBulbieJr (F1)

Ochi17 (F1)

OfUltron (F1)

Ogopogo (F1)

OldBen (F1)

OlympiaSaint (F1)

Ovechkin (F1)

OwlsT2W (F1)

Pacc40 (F1)

Peanut21s (F1)

Phabio (F1)

PHappiness (F1)

Phasih (F1)

Phatniss (F1)

PhrankthaTank (F1)

Piper2020 (F1)

Pippy (F1)

Plackberry (F1)

Plumbus (F1)

PMC (F1)

Poenanya (F1)

Polka14 (F1)

Pollywog (F1)

PopTart (F1)

Priscilla (F1)

Prometheus (F1)

QuickMath (F1)

Quico (F1)

Ramsey (F1)

RitaG (F1)

Rocktrap (F1)

RockyHorror (F1)

Rombolo (F1)

Royals2015 (F1)

Ruby (F1)

S2Kari (F1)

S2TSquared (F1)

Saal (F1)

SaltyMike (F1)

Sandalphon (F1)

SassyB (F1)

Scottish (F1)

Seagreen (F1)

SG4 (F1)

Sharpshooter (F1)

Shauna1 (F1)

SherL (F1)

ShiLan (F1)

SimranZ1 (F1)

SiSi (F1)

Sparkdehlily (F1)

Spartacus (F1)

Spikelee (F1)

Spoonbill (F1)

Strokeseat (F1)

SuperGrey (F1)

SwagPigglett (F1)

Taj (F1)

Taus (F1)

TDanisky (F1)

TonaldTDrump (F1)

TootsiePop (F1)

Tweety (F1)

UncleRicky (F1)

Velveteen (F1)

Wachhund (F1)

Wee (F1)

WheatBerry18 (F1)

Whouxphf (F1)

WillSterrel (F1)

WIVsmall (F1)

XFactor (F1)

Zerg (F1)

Avani (F2)

Che9d (F2)

Demsculpinboyz (F2)

Jabbawokkie (F2)

Yoshi (F2)

Zapner (F2)

Squirty (F3)

TChen (F4)

ThetaBob (F4)

Cornie (F5)

Annotation StatusIn GenBank
Plaque NotesPlaques are very clear and show up very quickly. the phage has a lytic cycle and will create a web plate withing 24 go 30 hours. there isn't any variation except that the plaques became more and more clear as I purified it. After dilution 3, the morphology has stayed the same each time.
MorphotypeSiphoviridae
Number of Genes107
Has been Phamerated?Yes
Gene List

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Shauna1_1
(terminase small subunit)

Shauna1_2
(terminase large subunit)

Shauna1_3
(portal protein)

Shauna1_4
(capsid maturation protease)

Shauna1_5
(scaffolding protein)

Shauna1_6
(major capsid protein)

Shauna1_7
(head-to-tail connector complex protein)

Shauna1_8

Shauna1_9
(head-to-tail connector complex protein)

Shauna1_10
(head-to-tail connector complex protein)

Shauna1_11
(major tail subunit)

Shauna1_12
(tail assembly chaperone)

Shauna1_13
(tail assembly chaperone)

Shauna1_14
(tapemeasure protein)

Shauna1_15
(minor tail subunit)

Shauna1_16
(minor tail subunit)

Shauna1_17
(minor tail subunit)

Shauna1_18
(minor tail subunit)

Shauna1_19
(minor tail protein)

Shauna1_20

Shauna1_21

Shauna1_22

Shauna1_23

Shauna1_24

Shauna1_25

Shauna1_26

Shauna1_27
(lysin A)

Shauna1_28
(lysin B)

Shauna1_29
(holin)

Shauna1_30

Shauna1_31

Shauna1_32
(DnaQ-like exonuclease)

Shauna1_33

Shauna1_34

Shauna1_35

Shauna1_36

Shauna1_37

Shauna1_38
(integrase)

Shauna1_39

Shauna1_40
(immunity repressor)

Shauna1_41
(HTH DNA binding domain protein)

Shauna1_42

Shauna1_43

Shauna1_44

Shauna1_45

Shauna1_46

Shauna1_47

Shauna1_48

Shauna1_49

Shauna1_50

Shauna1_51

Shauna1_52

Shauna1_53

Shauna1_54

Shauna1_55

Shauna1_56
(WhiB family transcription factor)

Shauna1_57

Shauna1_58
(HTH DNA binding domain protein)

Shauna1_59

Shauna1_60

Shauna1_61

Shauna1_62

Shauna1_63
(HNH endonuclease domain protein)

Shauna1_64

Shauna1_65

Shauna1_66

Shauna1_67
(DNA methylase)

Shauna1_68

Shauna1_69

Shauna1_70

Shauna1_71

Shauna1_72
(DNA methylase)

Shauna1_73

Shauna1_74

Shauna1_75

Shauna1_76

Shauna1_77

Shauna1_78

Shauna1_79

Shauna1_80

Shauna1_81

Shauna1_82

Shauna1_83

Shauna1_84

Shauna1_85

Shauna1_86

Shauna1_87

Shauna1_88

Shauna1_89

Shauna1_90

Shauna1_91

Shauna1_92

Shauna1_93

Shauna1_94
(GIY-YIG endonuclease)

Shauna1_95

Shauna1_96

Shauna1_97

Shauna1_98

Shauna1_99

Shauna1_100

Shauna1_101

Shauna1_102

Shauna1_103
(galactosyltransferase)

Shauna1_104

Shauna1_105
(glucosyltransferase)

Shauna1_106
(HNH endonuclease domain protein)

Shauna1_107
(HNH endonuclease domain protein)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionJN020141
Refseq NumberNone yet
Published in PaperPope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status Archived
SEA Designator 2010BRYOshauna1MGS
Pitt Freezer Box# 11
Pitt Freezer Box Grid# 53
Available Files
Plaque PictureDownload
EM PictureDownload
XML FileDownload
GenBank File for PhameratorDownload