Mycobacterium phage James
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Detailed Information for Phage James
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByGarrett Santini
Year Found2015
Location FoundWayne, NJ, NJ USA
Finding InstitutionLehigh University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature-3°C
GPS Coordinates40.910833 N, 74.261667 W Map
Naming NotesDuring the processes of phage titer assays, this phage had acquired the nickname "The Great Plaquesby" which was inspired by F. Scott Fitzgerald's "The Great Gatsby." Based off of this nickname, the name James was chosen to represent James Gatsby from the novel. Since James is such an exciting phage, it couldn't be more fitting that his name comes out of a novel written about the roaring 1920s in America.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedAug 5, 2015
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1667
Genome length (bp)59617
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGACGGCGC
GC Content61.4%
Fasta file available?Yes: Download fasta file
Characterization
ClusterF
SubclusterF1
Cluster Life CycleTemperate
Other Cluster Members

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Alexphander (F1)

ArcusAngelus (F1)

Ardmore (F1)

Awesomesauce (F1)

Batiatus (F1)

BigPhil (F1)

Bipolar (F1)

Blexus (F1)

BobaPhett (F1)

Bobi (F1)

BodEinwohner17 (F1)

Boomer (F1)

Brocalys (F1)

Bubbles123 (F1)

Burwell21 (F1)

BuzzLyseyear (F1)

ByChance (F1)

Byougenkin (F1)

Cabrinians (F1)

CaptainTrips (F1)

Cerasum (F1)

Che8 (F1)

Chuckly (F1)

Clifton (F1)

Coco12 (F1)

Cornucopia (F1)

Daenerys (F1)

Dante (F1)

DaWorst (F1)

DeadP (F1)

DillTech15 (F1)

DirtMcgirt (F1)

DLane (F1)

Donkeykong (F1)

Doomphist (F1)

Dorothy (F1)

DotProduct (F1)

Doug (F1)

Drago (F1)

DRBy19 (F1)

Eish (F1)

EleanorGeorge (F1)

Emma (F1)

Empress (F1)

Enby (F1)

Estave1 (F1)

Fancypants (F1)

Filuzino (F1)

Firehouse51 (F1)

Flathead (F1)

Florinda (F1)

Frankie (F1)

Fruitloop (F1)

Galactic (F1)

Gandalph (F1)

Geralt (F1)

Girafales (F1)

Girr (F1)

Gorge (F1)

GUmbie (F1)

Hades (F1)

Hamulus (F1)

Harley (F1)

Hegedechwinu (F1)

Ibhubesi (F1)

Inventum (F1)

IrishSherpFalk (F1)

James (F1)

Job42 (F1)

JoeyJr (F1)

Juice456 (F1)

Kenuha5 (F1)

Kersh (F1)

Kimberlium (F1)

KingMidas (F1)

Kingsley (F1)

Koella (F1)

Krakatau (F1)

KristaRAM (F1)

LastJedi (F1)

Latretium (F1)

LilMoolah (F1)

Lizziana (F1)

Llama (F1)

Llij (F1)

Lorde (F1)

Mahavrat (F1)

Mantra (F1)

Mattes (F1)

Melissauren88 (F1)

MilleniumForce (F1)

MinionDave (F1)

Minnie (F1)

Misha28 (F1)

MisterCuddles (F1)

Modragons (F1)

Mozy (F1)

Mutaforma13 (F1)

Nimbo (F1)

Nitzel (F1)

Nivrat (F1)

NormanBulbieJr (F1)

Ochi17 (F1)

OfUltron (F1)

Ogopogo (F1)

OldBen (F1)

OlympiaSaint (F1)

Ovechkin (F1)

OwlsT2W (F1)

Pacc40 (F1)

Phanphagia (F1)

PHappiness (F1)

Phasih (F1)

Phatniss (F1)

Piper2020 (F1)

Pippy (F1)

Plumbus (F1)

PMC (F1)

Poenanya (F1)

Polka14 (F1)

Pollywog (F1)

PopTart (F1)

Priscilla (F1)

QuickMath (F1)

Quico (F1)

Ramsey (F1)

RitaG (F1)

RockyHorror (F1)

Royals2015 (F1)

Ruby (F1)

Saal (F1)

Sandalphon (F1)

SassyB (F1)

Scottish (F1)

Seabastian (F1)

Seagreen (F1)

SG4 (F1)

Shauna1 (F1)

ShiLan (F1)

SimranZ1 (F1)

SiSi (F1)

Sparkdehlily (F1)

Spartacus (F1)

Spikelee (F1)

Spoonbill (F1)

StAnnes (F1)

Strokeseat (F1)

SuperGrey (F1)

SwagPigglett (F1)

Taj (F1)

TDanisky (F1)

TootsiePop (F1)

Tweety (F1)

UncleRicky (F1)

Velveteen (F1)

Veteran (F1)

Wachhund (F1)

Wee (F1)

Whouxphf (F1)

WillSterrel (F1)

XFactor (F1)

Zerg (F1)

Avani (F2)

Che9d (F2)

Demsculpinboyz (F2)

Jabbawokkie (F2)

Yoshi (F2)

Zapner (F2)

Squirty (F3)

Renaud18 (F4)

TChen (F4)

ThetaBob (F4)

Cornie (F5)

Annotating InstitutionLehigh University
Annotation StatusIn GenBank
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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James_1
(terminase small subunit)

James_2
(terminase large subunit)

James_3
(portal protein)

James_4
(capsid maturation protease)

James_5
(scaffolding protein)

James_6
(major capsid protein)

James_7
(head-to-tail adaptor)

James_8
(head-to-tail stopper)

James_9

James_10
(tail terminator)

James_11
(major tail subunit)

James_12
(tail assembly chaperone)

James_13
(tail assembly chaperone)

James_14
(tape measure protein)

James_15
(minor tail protein)

James_16
(minor tail protein)

James_17
(minor tail protein)

James_18
(minor tail protein)

James_19
(minor tail protein)

James_20
(minor tail protein)

James_21
(minor tail protein)

James_22
(minor tail protein)

James_23
(minor tail protein)

James_24
(minor tail protein)

James_25
(helix-turn-helix DNA binding domain protein)

James_26

James_27

James_28

James_29

James_30
(lysin A)

James_31
(lysin B)

James_32
(holin)

James_33

James_34

James_35
(DNA polymerase III subunit)

James_36

James_37

James_38

James_39

James_40

James_41

James_42

James_43
(integrase)

James_44
(RelE-like toxin)

James_45
(RelB-like antitoxin)

James_46

James_47
(immunity repressor)

James_48

James_49
(helix-turn-helix DNA binding domain protein)

James_50
(antirepressor)

James_51

James_52

James_53

James_54
(helix-turn-helix DNA binding domain protein)

James_55

James_56

James_57

James_58

James_59

James_60

James_61
(WhiB family transcription factor)

James_62

James_63
(WhiB family transcription factor)

James_64
(helix-turn-helix DNA binding domain protein)

James_65

James_66

James_67

James_68
(AAA-ATPase)

James_69
(HNH endonuclease)

James_70
(DNA methylase)

James_71

James_72

James_73

James_74
(GIY-YIG homing endonuclease)

James_75

James_76

James_77

James_78

James_79

James_80

James_81

James_82

James_83

James_84
(MPME1 protein)

James_85

James_86

James_87

James_88

James_89

James_90

James_91

James_92

James_93

James_94

James_95
(GIY-YIG homing endonuclease)

James_96

James_97

James_98

James_99

James_100

James_101

James_102

James_103

James_104

James_105
(glycosyltransferase)

James_106

James_107
(glycosyltransferase)

James_108
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK524517
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 2x10^9
Pitt Freezer Box# 15
Pitt Freezer Box Grid# G7
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload