Mycobacterium phage ShroomBoi
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Detailed Information for Phage ShroomBoi
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByJulia Shelton
Year Found2022
Location FoundBlacksburg, VA USA
Finding InstitutionVirginia Tech
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature37°C
GPS Coordinates37.213 N, 80.445 W Map
Discovery NotesWas found in an indoor cactus plant with mushroom growth.
Naming NotesThe soil had some fungus growth, so I named it after the mushrooms.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 4, 2023
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1201
Genome length (bp)58221
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGACGGCGC
GC Content61.4%
Fasta file available?Yes: Download fasta file
Characterization
ClusterF
SubclusterF1
Cluster Life CycleTemperate
Other Cluster Members

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Blarby (None)

MulchSalad (None)

Akhila (F1)

Alexphander (F1)

AlpineSix (F1)

ArcusAngelus (F1)

Ardmore (F1)

Aubs (F1)

Awesomesauce (F1)

Batiatus (F1)

Beakin (F1)

BigPhil (F1)

Bipolar (F1)

Blexus (F1)

BlueCrab (F1)

BobaPhett (F1)

Bobi (F1)

BodEinwohner17 (F1)

Boomer (F1)

Brocalys (F1)

Bubbles123 (F1)

Burwell21 (F1)

BuzzLyseyear (F1)

ByChance (F1)

Byougenkin (F1)

Cabrinians (F1)

CaptainTrips (F1)

Cerasum (F1)

Che8 (F1)

Chevrolet (F1)

ChickenDinner (F1)

Chuckly (F1)

Clifton (F1)

Coco12 (F1)

Cornucopia (F1)

Crumble (F1)

DaddyRickover (F1)

Daenerys (F1)

Dante (F1)

DaWorst (F1)

DeadP (F1)

Deb65 (F1)

DillTech15 (F1)

DirtMcgirt (F1)

DLane (F1)

DocMcStuffins (F1)

Donkeykong (F1)

Doomphist (F1)

Dorothy (F1)

DosHalletts (F1)

DotProduct (F1)

Doug (F1)

Drago (F1)

DRBy19 (F1)

Eish (F1)

EleanorGeorge (F1)

Emma (F1)

Empress (F1)

Enby (F1)

Eradicator (F1)

Estave1 (F1)

Fancypants (F1)

Fastidio (F1)

Filuzino (F1)

Firehouse51 (F1)

Flathead (F1)

Florinda (F1)

Frankie (F1)

FreddyB (F1)

Fruitloop (F1)

Galactic (F1)

Gandalph (F1)

Geralt (F1)

GigiOuiOui (F1)

Girafales (F1)

Girr (F1)

Gorge (F1)

GUmbie (F1)

Guppsters (F1)

Hades (F1)

Hamulus (F1)

Harley (F1)

Hegedechwinu (F1)

Hlubikazi (F1)

Ibhubesi (F1)

IbOuu (F1)

IMpilo (F1)

Inventum (F1)

IrishSherpFalk (F1)

JalFarm20 (F1)

James (F1)

Jarcob (F1)

Jinglebell (F1)

Job42 (F1)

JoeyJr (F1)

Juice456 (F1)

Juniper1 (F1)

Karhdo (F1)

Kenuha5 (F1)

Kersh (F1)

Kimberlium (F1)

KingMidas (F1)

Kingsley (F1)

Koella (F1)

Krakatau (F1)

KristaRAM (F1)

LastJedi (F1)

Latretium (F1)

Leozinho (F1)

Leperchaun (F1)

LilMoolah (F1)

LittleShirley (F1)

Lizziana (F1)

Llama (F1)

Llij (F1)

Lorde (F1)

LunaBlu (F1)

Madiba (F1)

MadMen (F1)

Mahavrat (F1)

Mandlovu (F1)

Mantra (F1)

Maravista (F1)

Marker (F1)

Martik (F1)

Mattes (F1)

Melissauren88 (F1)

MilanaBonita (F1)

MilleniumForce (F1)

MinionDave (F1)

Minnie (F1)

Misha28 (F1)

MisterCuddles (F1)

Modragons (F1)

Moonbeam (F1)

Mova (F1)

Mozy (F1)

Ms6 (F1)

MulchExplorer (F1)

Mutaforma13 (F1)

Nimbo (F1)

Nitzel (F1)

Nivrat (F1)

NormanBulbieJr (F1)

Ochi17 (F1)

OfUltron (F1)

Ogopogo (F1)

Oksu (F1)

OldBen (F1)

OlympiaSaint (F1)

Ovechkin (F1)

OwlsT2W (F1)

Pacc40 (F1)

Phalconet (F1)

Phanphagia (F1)

PHappiness (F1)

Phasih (F1)

Phatniss (F1)

PhesterPhotato (F1)

Philus (F1)

Piper2020 (F1)

Pippy (F1)

Plumbus (F1)

PMC (F1)

Poenanya (F1)

Polka14 (F1)

Pollywog (F1)

PopTart (F1)

Priscilla (F1)

QuickMath (F1)

Quico (F1)

Radiance (F1)

Ramsey (F1)

RedBird (F1)

Rialto (F1)

Rita (F1)

RitaG (F1)

Rockne (F1)

RockyHorror (F1)

Royals2015 (F1)

Ruby (F1)

Saal (F1)

Sabbb (F1)

Sandalphon (F1)

Sarma624 (F1)

Sassafras (F1)

SassyB (F1)

Scottish (F1)

Seabastian (F1)

Seagreen (F1)

SG4 (F1)

Shauna1 (F1)

ShiLan (F1)

ShowerHandel (F1)

ShroomBoi (F1)

SimranZ1 (F1)

SiSi (F1)

Sparkdehlily (F1)

Spartacus (F1)

Spikelee (F1)

Spoonbill (F1)

StAnnes (F1)

Stap (F1)

Starcevich (F1)

Strokeseat (F1)

SuperGrey (F1)

SwagPigglett (F1)

Taj (F1)

TDanisky (F1)

TootsiePop (F1)

Tootsieroll (F1)

Totinger (F1)

Tweety (F1)

UncleRicky (F1)

Velveteen (F1)

Veteran (F1)

VRedHorse (F1)

Wachhund (F1)

Wee (F1)

Whatsapiecost (F1)

Whouxphf (F1)

WillSterrel (F1)

XFactor (F1)

Yorick (F1)

Zerg (F1)

Zizzle (F1)

Avani (F2)

Che9d (F2)

Demsculpinboyz (F2)

Jabbawokkie (F2)

Soul22 (F2)

Yoshi (F2)

Zapner (F2)

Squirty (F3)

LunaStella (F4)

Renaud18 (F4)

TChen (F4)

ThetaBob (F4)

Cornie (F5)

Toron (F6)

Annotating InstitutionVirginia Tech
Annotation StatusIn GenBank
Plaque NotesLarge, translucent plaques
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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ShroomBoi_1
(terminase small subunit)

ShroomBoi_2
(terminase large subunit)

ShroomBoi_3
(portal protein)

ShroomBoi_4
(capsid maturation protease)

ShroomBoi_5
(scaffolding protein)

ShroomBoi_6
(major capsid protein)

ShroomBoi_7
(head-to-tail adaptor)

ShroomBoi_8
(head-to-tail stopper)

ShroomBoi_9

ShroomBoi_10
(tail terminator)

ShroomBoi_11
(major tail protein)

ShroomBoi_12
(tail assembly chaperone)

ShroomBoi_13
(tail assembly chaperone)

ShroomBoi_14
(tape measure protein)

ShroomBoi_15
(minor tail protein)

ShroomBoi_16
(minor tail protein)

ShroomBoi_17

ShroomBoi_18
(minor tail protein)

ShroomBoi_19

ShroomBoi_20

ShroomBoi_21

ShroomBoi_22

ShroomBoi_23

ShroomBoi_24

ShroomBoi_25

ShroomBoi_26

ShroomBoi_27
(lysin A)

ShroomBoi_28
(lysin B)

ShroomBoi_29
(holin)

ShroomBoi_30

ShroomBoi_31

ShroomBoi_32
(DnaQ-like DNA polymerase III subunit)

ShroomBoi_33

ShroomBoi_34

ShroomBoi_35

ShroomBoi_36

ShroomBoi_37

ShroomBoi_38

ShroomBoi_39

ShroomBoi_40
(helix-turn-helix DNA binding domain protein)

ShroomBoi_41

ShroomBoi_42
(integrase)

ShroomBoi_43

ShroomBoi_44

ShroomBoi_45
(immunity repressor)

ShroomBoi_46
(Cro protein)

ShroomBoi_47
(antirepressor)

ShroomBoi_48

ShroomBoi_49

ShroomBoi_50
(excise)

ShroomBoi_51

ShroomBoi_52

ShroomBoi_53

ShroomBoi_54

ShroomBoi_55

ShroomBoi_56

ShroomBoi_57

ShroomBoi_58

ShroomBoi_59
(WhiB family transcription factor)

ShroomBoi_60

ShroomBoi_61
(WhiB family transcription factor)

ShroomBoi_62
(helix-turn-helix DNA binding domain protein)

ShroomBoi_63

ShroomBoi_64

ShroomBoi_65

ShroomBoi_66
(SSB protein)

ShroomBoi_67
(DNA methyltransferase)

ShroomBoi_68

ShroomBoi_69

ShroomBoi_70

ShroomBoi_71
(DNA methyltransferase)

ShroomBoi_72

ShroomBoi_73

ShroomBoi_74

ShroomBoi_75
(RepA-like replication initiator)

ShroomBoi_76

ShroomBoi_77

ShroomBoi_78

ShroomBoi_79

ShroomBoi_80

ShroomBoi_81

ShroomBoi_82

ShroomBoi_83

ShroomBoi_84

ShroomBoi_85

ShroomBoi_86

ShroomBoi_87

ShroomBoi_88

ShroomBoi_89

ShroomBoi_90

ShroomBoi_91

ShroomBoi_92

ShroomBoi_93

ShroomBoi_94

ShroomBoi_95

ShroomBoi_96

ShroomBoi_97

ShroomBoi_98

ShroomBoi_99
(glycosyltransferase)

ShroomBoi_100
(kinase)

ShroomBoi_101

ShroomBoi_102

ShroomBoi_103

ShroomBoi_104
(glycosyltransferase)

ShroomBoi_105
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionOR159667
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 153
Pitt Freezer Box Grid# G3
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload