Mycobacterium phage DRBy19
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Detailed Information for Phage DRBy19
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByMia Wenzel
Year Found2015
Location FoundHarrisonburg, VA USA
Finding InstitutionJames Madison University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Unknown
Isolation TemperatureNot entered
GPS Coordinates38.426439 N, 78.859958 W Map
Naming NotesI play roller derby, and my number is #19. So I chose to name my phage DRBy19, a small piece of who I am right now!
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 13, 2016
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1701
Genome length (bp)58368
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGAAGGCGC
GC Content61.3%
Sequencing NotesThere was a contaminating phage, present in ~10% of reads, that was another Cluster F1 phage.

In addition, this phage has a number of apparent SNPs. They are:
Positions 18863-4, ~38% of reads call GA instead of consensus CC.
Position 20123, ~43% of reads call T instead of consensus G.
Position 53499, ~48% of reads call C instead of consensus A.
Position 53646, ~38% of reads call C instead of consensus T.
Position 53715, ~50% of reads call C instead of consensus T.
Fasta file available?Yes: Download fasta file
Characterization
ClusterF
SubclusterF1
Cluster Life CycleTemperate
Other Cluster Members

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Soul22 (None)

Akhila (F1)

Alexphander (F1)

ArcusAngelus (F1)

Ardmore (F1)

Awesomesauce (F1)

Batiatus (F1)

BigPhil (F1)

Bipolar (F1)

Blexus (F1)

BobaPhett (F1)

Bobi (F1)

BodEinwohner17 (F1)

Boomer (F1)

Brocalys (F1)

Bubbles123 (F1)

Burwell21 (F1)

BuzzLyseyear (F1)

ByChance (F1)

Byougenkin (F1)

Cabrinians (F1)

CaptainTrips (F1)

Cerasum (F1)

Che8 (F1)

Chuckly (F1)

Clifton (F1)

Coco12 (F1)

Cornucopia (F1)

Daenerys (F1)

Dante (F1)

DaWorst (F1)

DeadP (F1)

DillTech15 (F1)

DirtMcgirt (F1)

DLane (F1)

Donkeykong (F1)

Doomphist (F1)

Dorothy (F1)

DotProduct (F1)

Doug (F1)

Drago (F1)

DRBy19 (F1)

Eish (F1)

EleanorGeorge (F1)

Emma (F1)

Empress (F1)

Enby (F1)

Eradicator (F1)

Estave1 (F1)

Fancypants (F1)

Filuzino (F1)

Firehouse51 (F1)

Flathead (F1)

Florinda (F1)

Frankie (F1)

Fruitloop (F1)

Galactic (F1)

Gandalph (F1)

Geralt (F1)

Girafales (F1)

Girr (F1)

Gorge (F1)

GUmbie (F1)

Hades (F1)

Hamulus (F1)

Harley (F1)

Hegedechwinu (F1)

Hlubikazi (F1)

Ibhubesi (F1)

Inventum (F1)

IrishSherpFalk (F1)

James (F1)

Job42 (F1)

JoeyJr (F1)

Juice456 (F1)

Kenuha5 (F1)

Kersh (F1)

Kimberlium (F1)

KingMidas (F1)

Kingsley (F1)

Koella (F1)

Krakatau (F1)

KristaRAM (F1)

LastJedi (F1)

Latretium (F1)

LilMoolah (F1)

Lizziana (F1)

Llama (F1)

Llij (F1)

Lorde (F1)

Mahavrat (F1)

Mandlovu (F1)

Mantra (F1)

Martik (F1)

Mattes (F1)

Melissauren88 (F1)

MilanaBonita (F1)

MilleniumForce (F1)

MinionDave (F1)

Minnie (F1)

Misha28 (F1)

MisterCuddles (F1)

Modragons (F1)

Moonbeam (F1)

Mova (F1)

Mozy (F1)

Mutaforma13 (F1)

Nimbo (F1)

Nitzel (F1)

Nivrat (F1)

NormanBulbieJr (F1)

Ochi17 (F1)

OfUltron (F1)

Ogopogo (F1)

OldBen (F1)

OlympiaSaint (F1)

Ovechkin (F1)

OwlsT2W (F1)

Pacc40 (F1)

Phanphagia (F1)

PHappiness (F1)

Phasih (F1)

Phatniss (F1)

Piper2020 (F1)

Pippy (F1)

Plumbus (F1)

PMC (F1)

Poenanya (F1)

Polka14 (F1)

Pollywog (F1)

PopTart (F1)

Priscilla (F1)

QuickMath (F1)

Quico (F1)

Ramsey (F1)

RitaG (F1)

RockyHorror (F1)

Royals2015 (F1)

Ruby (F1)

Saal (F1)

Sandalphon (F1)

Sassafras (F1)

SassyB (F1)

Scottish (F1)

Seabastian (F1)

Seagreen (F1)

SG4 (F1)

Shauna1 (F1)

ShiLan (F1)

SimranZ1 (F1)

SiSi (F1)

Sparkdehlily (F1)

Spartacus (F1)

Spikelee (F1)

Spoonbill (F1)

StAnnes (F1)

Strokeseat (F1)

SuperGrey (F1)

SwagPigglett (F1)

Taj (F1)

TDanisky (F1)

TootsiePop (F1)

Tweety (F1)

UncleRicky (F1)

Velveteen (F1)

Veteran (F1)

Wachhund (F1)

Wee (F1)

Whouxphf (F1)

WillSterrel (F1)

XFactor (F1)

Zerg (F1)

Avani (F2)

Che9d (F2)

Demsculpinboyz (F2)

Jabbawokkie (F2)

Yoshi (F2)

Zapner (F2)

Squirty (F3)

Renaud18 (F4)

TChen (F4)

ThetaBob (F4)

Cornie (F5)

Annotating InstitutionUniversity of California, Los Angeles
Annotation StatusIn GenBank
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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DRBy19_1
(terminase small subunit)

DRBy19_2
(terminase large subunit)

DRBy19_3
(portal protein)

DRBy19_4
(capsid maturation protease and MuF-like fusion protein)

DRBy19_5
(scaffolding protein)

DRBy19_6
(major capsid protein)

DRBy19_7
(head-to-tail adaptor)

DRBy19_8
(head-to-tail stopper)

DRBy19_9

DRBy19_10
(tail terminator)

DRBy19_11
(major tail protein)

DRBy19_12
(tail assembly chaperone)

DRBy19_13
(tail assembly chaperone)

DRBy19_14
(tape measure protein)

DRBy19_15
(minor tail protein)

DRBy19_16
(minor tail protein)

DRBy19_17
(minor tail protein)

DRBy19_18
(minor tail protein)

DRBy19_19
(minor tail protein)

DRBy19_20
(minor tail protein)

DRBy19_21
(minor tail protein)

DRBy19_22
(minor tail protein)

DRBy19_23
(minor tail protein)

DRBy19_24
(minor tail protein)

DRBy19_25
(helix-turn-helix DNA binding domain protein)

DRBy19_26

DRBy19_27

DRBy19_28

DRBy19_29

DRBy19_30
(lysin A)

DRBy19_31
(lysin B)

DRBy19_32
(holin)

DRBy19_33

DRBy19_34

DRBy19_35
(DNA polymerase III subunit)

DRBy19_36

DRBy19_37

DRBy19_38

DRBy19_39

DRBy19_40

DRBy19_41

DRBy19_42

DRBy19_43
(tyrosine integrase)

DRBy19_44
(RelE-like toxin)

DRBy19_45
(RelB-like antitoxin)

DRBy19_46

DRBy19_47
(immunity repressor)

DRBy19_48
(Cro protein)

DRBy19_49
(antirepressor)

DRBy19_50

DRBy19_51

DRBy19_52

DRBy19_53

DRBy19_54
(excise)

DRBy19_55

DRBy19_56

DRBy19_57

DRBy19_58

DRBy19_59

DRBy19_60
(WhiB family transcription factor)

DRBy19_61
(helix-turn-helix DNA binding domain protein)

DRBy19_62
(WhiB family transcription factor)

DRBy19_63
(helix-turn-helix DNA binding domain protein)

DRBy19_64

DRBy19_65

DRBy19_66

DRBy19_67
(helix-turn-helix DNA binding domain protein)

DRBy19_68
(RecA-like DNA recombinase)

DRBy19_69
(HNH endonuclease)

DRBy19_70
(helix-turn-helix DNA binding domain protein)

DRBy19_71

DRBy19_72
(DNA methylase)

DRBy19_73

DRBy19_74

DRBy19_75

DRBy19_76

DRBy19_77
(G-I-Y Y-I-G endonuclease)

DRBy19_78
(helix-turn-helix DNA binding domain protein)

DRBy19_79

DRBy19_80

DRBy19_81

DRBy19_82

DRBy19_83

DRBy19_84

DRBy19_85

DRBy19_86

DRBy19_87
(MPME1 protein)

DRBy19_88

DRBy19_89

DRBy19_90

DRBy19_91

DRBy19_92

DRBy19_93

DRBy19_94
(G-I-Y Y-I-G endonuclease)

DRBy19_95

DRBy19_96

DRBy19_97

DRBy19_98

DRBy19_99

DRBy19_100

DRBy19_101
(glycosyltransferase)

DRBy19_102

DRBy19_103
(glycosyltransferase)

DRBy19_104
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMT310893
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 4e6
Pitt Freezer Box# 27
Pitt Freezer Box Grid# C8
Available Files
GenBank File for PhameratorDownload