Mycobacterium phage Madiba
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Detailed Information for Phage Madiba
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByDeborah Jacobs-Sera
Year Found2018
Location FoundDurban, South Africa
Finding InstitutionUniversity of Pittsburgh
ProgramPhage Hunters Integrating Research and Education
From enriched soil sample?Yes
Isolation Temperature37°C
GPS Coordinates29.838968 S, 30.996173 E Map
Discovery NotesFound in the front yard of St. Anne's Guest House in Durban.
Naming Notes"Madiba"is a Southern African title of respect for Nelson Mandela, derived from his Xhosa clan name.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedAug 19, 2021
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1249
Genome length (bp)56056
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCCGATGGCAT
GC Content61.4%
Fasta file available?Yes: Download fasta file
Characterization
ClusterF
SubclusterF1
Cluster Life CycleTemperate
Lysogeny NotesColonies formed in clearing from spotted lysate.
Other Cluster Members

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Soul22 (None)

Akhila (F1)

Alexphander (F1)

ArcusAngelus (F1)

Ardmore (F1)

Aubs (F1)

Awesomesauce (F1)

Batiatus (F1)

Beakin (F1)

BigPhil (F1)

Bipolar (F1)

Blexus (F1)

BobaPhett (F1)

Bobi (F1)

BodEinwohner17 (F1)

Boomer (F1)

Brocalys (F1)

Bubbles123 (F1)

Burwell21 (F1)

BuzzLyseyear (F1)

ByChance (F1)

Byougenkin (F1)

Cabrinians (F1)

CaptainTrips (F1)

Cerasum (F1)

Che8 (F1)

Chuckly (F1)

Clifton (F1)

Coco12 (F1)

Cornucopia (F1)

Daenerys (F1)

Dante (F1)

DaWorst (F1)

DeadP (F1)

DillTech15 (F1)

DirtMcgirt (F1)

DLane (F1)

Donkeykong (F1)

Doomphist (F1)

Dorothy (F1)

DotProduct (F1)

Doug (F1)

Drago (F1)

DRBy19 (F1)

Eish (F1)

EleanorGeorge (F1)

Emma (F1)

Empress (F1)

Enby (F1)

Eradicator (F1)

Estave1 (F1)

Fancypants (F1)

Filuzino (F1)

Firehouse51 (F1)

Flathead (F1)

Florinda (F1)

Frankie (F1)

Fruitloop (F1)

Galactic (F1)

Gandalph (F1)

Geralt (F1)

GigiOuiOui (F1)

Girafales (F1)

Girr (F1)

Gorge (F1)

GUmbie (F1)

Hades (F1)

Hamulus (F1)

Harley (F1)

Hegedechwinu (F1)

Hlubikazi (F1)

Ibhubesi (F1)

Inventum (F1)

IrishSherpFalk (F1)

JalFarm20 (F1)

James (F1)

Jarcob (F1)

Job42 (F1)

JoeyJr (F1)

Juice456 (F1)

Kenuha5 (F1)

Kersh (F1)

Kimberlium (F1)

KingMidas (F1)

Kingsley (F1)

Koella (F1)

Krakatau (F1)

KristaRAM (F1)

LastJedi (F1)

Latretium (F1)

Leozinho (F1)

Leperchaun (F1)

LilMoolah (F1)

Lizziana (F1)

Llama (F1)

Llij (F1)

Lorde (F1)

Madiba (F1)

MadMen (F1)

Mahavrat (F1)

Mandlovu (F1)

Mantra (F1)

Martik (F1)

Mattes (F1)

Melissauren88 (F1)

MilanaBonita (F1)

MilleniumForce (F1)

MinionDave (F1)

Minnie (F1)

Misha28 (F1)

MisterCuddles (F1)

Modragons (F1)

Moonbeam (F1)

Mova (F1)

Mozy (F1)

Mutaforma13 (F1)

Nimbo (F1)

Nitzel (F1)

Nivrat (F1)

NormanBulbieJr (F1)

Ochi17 (F1)

OfUltron (F1)

Ogopogo (F1)

OldBen (F1)

OlympiaSaint (F1)

Ovechkin (F1)

OwlsT2W (F1)

Pacc40 (F1)

Phalconet (F1)

Phanphagia (F1)

PHappiness (F1)

Phasih (F1)

Phatniss (F1)

Piper2020 (F1)

Pippy (F1)

Plumbus (F1)

PMC (F1)

Poenanya (F1)

Polka14 (F1)

Pollywog (F1)

PopTart (F1)

Priscilla (F1)

QuickMath (F1)

Quico (F1)

Ramsey (F1)

Rita (F1)

RitaG (F1)

Rockne (F1)

RockyHorror (F1)

Royals2015 (F1)

Ruby (F1)

Saal (F1)

Sabbb (F1)

Sandalphon (F1)

Sarma624 (F1)

Sassafras (F1)

SassyB (F1)

Scottish (F1)

Seabastian (F1)

Seagreen (F1)

SG4 (F1)

Shauna1 (F1)

ShiLan (F1)

SimranZ1 (F1)

SiSi (F1)

Sparkdehlily (F1)

Spartacus (F1)

Spikelee (F1)

Spoonbill (F1)

StAnnes (F1)

Strokeseat (F1)

SuperGrey (F1)

SwagPigglett (F1)

Taj (F1)

TDanisky (F1)

TootsiePop (F1)

Tootsieroll (F1)

Tweety (F1)

UncleRicky (F1)

Velveteen (F1)

Veteran (F1)

VRedHorse (F1)

Wachhund (F1)

Wee (F1)

Whatsapiecost (F1)

Whouxphf (F1)

WillSterrel (F1)

XFactor (F1)

Yorick (F1)

Zerg (F1)

Zizzle (F1)

Avani (F2)

Che9d (F2)

Demsculpinboyz (F2)

Jabbawokkie (F2)

Yoshi (F2)

Zapner (F2)

Squirty (F3)

Renaud18 (F4)

TChen (F4)

ThetaBob (F4)

Cornie (F5)

Annotating InstitutionUniversity of Pittsburgh
Annotation StatusIn GenBank
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Madiba_1
(HNH endonuclease)

Madiba_2

Madiba_3
(terminase)

Madiba_4
(portal protein)

Madiba_5
(capsid maturation protease)

Madiba_6
(scaffolding protein)

Madiba_7
(major capsid protein)

Madiba_8
(head-to-tail adaptor)

Madiba_9
(head-to-tail stopper)

Madiba_10

Madiba_11
(tail terminator)

Madiba_12
(major tail protein)

Madiba_13
(tail assembly chaperone)

Madiba_14
(tail assembly chaperone)

Madiba_15
(tape measure protein)

Madiba_16
(minor tail protein)

Madiba_17
(minor tail protein)

Madiba_18
(minor tail protein)

Madiba_19
(minor tail protein)

Madiba_20
(minor tail protein)

Madiba_21
(minor tail protein)

Madiba_22

Madiba_23
(helix-turn-helix DNA binding protein)

Madiba_24

Madiba_25

Madiba_26

Madiba_27

Madiba_28
(lysin A)

Madiba_29
(lysin B)

Madiba_30

Madiba_31

Madiba_32

Madiba_33
(DnaQ-like DNA polymerase III subunit)

Madiba_34

Madiba_35

Madiba_36

Madiba_37

Madiba_38

Madiba_39
(tyrosine integrase)

Madiba_40

Madiba_41
(immunity repressor)

Madiba_42
(Cro protein)

Madiba_43
(antirepressor)

Madiba_44

Madiba_45

Madiba_46
(excise)

Madiba_47

Madiba_48

Madiba_49

Madiba_50

Madiba_51

Madiba_52

Madiba_53

Madiba_54

Madiba_55
(WhiB family transcription factor)

Madiba_56
(helix-turn-helix DNA binding protein)

Madiba_57
(WhiB family transcription factor)

Madiba_58
(helix-turn-helix DNA binding protein)

Madiba_59

Madiba_60

Madiba_61

Madiba_62

Madiba_63

Madiba_64

Madiba_65
(ssDNA binding protein)

Madiba_66
(HNH endonuclease)

Madiba_67
(DNA methylase)

Madiba_68

Madiba_69

Madiba_70

Madiba_71
(DNA methylase)

Madiba_72
(ribbon-helix-helix DNA binding domain protein)

Madiba_73

Madiba_74

Madiba_75

Madiba_76

Madiba_77
(RepA-like replication initiator)

Madiba_78
(helicase loader)

Madiba_79

Madiba_80

Madiba_81

Madiba_82

Madiba_83

Madiba_84

Madiba_85

Madiba_86

Madiba_87

Madiba_88

Madiba_89

Madiba_90

Madiba_91

Madiba_92

Madiba_93
(MeR-like helix-turn-helix DNA binding domain protein)

Madiba_94

Madiba_95

Madiba_96

Madiba_97

Madiba_98

Madiba_99

Madiba_100
(glycosyltransferase)

Madiba_101

Madiba_102
(glycosyltransferase)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionOM105885
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 96
Pitt Freezer Box Grid# C2
Available Files
Plaque PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload