Mycobacterium phage Misha28
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Detailed Information for Phage Misha28
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByMadeline Manfra-Levitt
Year Found2016
Location FoundCharlton, MA USA
Finding InstitutionWorcester Polytechnic Institute
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Unknown
Isolation TemperatureNot entered
GPS Coordinates42.120573 N, 71.98548 W Map
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedFeb 22, 2017
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1969
Genome length (bp)57455
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCCGAAGGCAT
GC Content62.9%
Sequencing Notes1 nucleotide different than TootsiePop, isolated from same sample
Fasta file available?Yes: Download fasta file
Characterization
ClusterF
SubclusterF1
Cluster Life CycleTemperate
Other Cluster Members

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Renaud18 (None)

Alexphander (F1)

ArcusAngelus (F1)

Ardmore (F1)

Awesomesauce (F1)

Batiatus (F1)

BigPhil (F1)

Bipolar (F1)

Blexus (F1)

BobaPhett (F1)

Bobi (F1)

BodEinwohner17 (F1)

Boomer (F1)

Brocalys (F1)

Bubbles123 (F1)

Burwell21 (F1)

BuzzLyseyear (F1)

ByChance (F1)

Byougenkin (F1)

Cabrinians (F1)

CaptainTrips (F1)

Cerasum (F1)

Che8 (F1)

Chuckly (F1)

Clifton (F1)

Coco12 (F1)

Cornucopia (F1)

Daenerys (F1)

Dante (F1)

DaWorst (F1)

DeadP (F1)

DillTech15 (F1)

DirtMcgirt (F1)

DLane (F1)

Donkeykong (F1)

Doomphist (F1)

Dorothy (F1)

DotProduct (F1)

Doug (F1)

Drago (F1)

DRBy19 (F1)

Eish (F1)

EleanorGeorge (F1)

Emma (F1)

Empress (F1)

Enby (F1)

Estave1 (F1)

Fancypants (F1)

Filuzino (F1)

Firehouse51 (F1)

Flathead (F1)

Florinda (F1)

Frankie (F1)

Fruitloop (F1)

Galactic (F1)

Gandalph (F1)

Geralt (F1)

Girafales (F1)

Girr (F1)

Gorge (F1)

GUmbie (F1)

Hades (F1)

Hamulus (F1)

Harley (F1)

Hegedechwinu (F1)

Ibhubesi (F1)

Inventum (F1)

IrishSherpFalk (F1)

James (F1)

Job42 (F1)

JoeyJr (F1)

Juice456 (F1)

Kenuha5 (F1)

Kersh (F1)

Kimberlium (F1)

KingMidas (F1)

Kingsley (F1)

Koella (F1)

Krakatau (F1)

KristaRAM (F1)

LastJedi (F1)

Latretium (F1)

LilMoolah (F1)

Lizziana (F1)

Llama (F1)

Llij (F1)

Lorde (F1)

Mahavrat (F1)

Mantra (F1)

Mattes (F1)

Melissauren88 (F1)

MilleniumForce (F1)

MinionDave (F1)

Minnie (F1)

Misha28 (F1)

MisterCuddles (F1)

Modragons (F1)

Mozy (F1)

Mutaforma13 (F1)

Nimbo (F1)

Nitzel (F1)

Nivrat (F1)

NormanBulbieJr (F1)

Ochi17 (F1)

OfUltron (F1)

Ogopogo (F1)

OldBen (F1)

OlympiaSaint (F1)

Ovechkin (F1)

OwlsT2W (F1)

Pacc40 (F1)

Phanphagia (F1)

PHappiness (F1)

Phasih (F1)

Phatniss (F1)

Piper2020 (F1)

Pippy (F1)

Plumbus (F1)

PMC (F1)

Poenanya (F1)

Polka14 (F1)

Pollywog (F1)

PopTart (F1)

Priscilla (F1)

QuickMath (F1)

Quico (F1)

Ramsey (F1)

RitaG (F1)

RockyHorror (F1)

Royals2015 (F1)

Ruby (F1)

Saal (F1)

Sandalphon (F1)

SassyB (F1)

Scottish (F1)

Seabastian (F1)

Seagreen (F1)

SG4 (F1)

Shauna1 (F1)

ShiLan (F1)

SimranZ1 (F1)

SiSi (F1)

Sparkdehlily (F1)

Spartacus (F1)

Spikelee (F1)

Spoonbill (F1)

StAnnes (F1)

Strokeseat (F1)

SuperGrey (F1)

SwagPigglett (F1)

Taj (F1)

TDanisky (F1)

TootsiePop (F1)

Tweety (F1)

UncleRicky (F1)

Velveteen (F1)

Veteran (F1)

Wachhund (F1)

Wee (F1)

Whouxphf (F1)

WillSterrel (F1)

XFactor (F1)

Zerg (F1)

Avani (F2)

Che9d (F2)

Demsculpinboyz (F2)

Jabbawokkie (F2)

Yoshi (F2)

Zapner (F2)

Squirty (F3)

TChen (F4)

ThetaBob (F4)

Cornie (F5)

Annotating InstitutionWorcester Polytechnic Institute
Annotation StatusIn GenBank
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Misha28_1
(HNH endonuclease)

Misha28_2
(terminase small subunit)

Misha28_3
(terminase large subunit)

Misha28_4
(portal protein)

Misha28_5
(capsid maturation protease)

Misha28_6
(scaffolding protein)

Misha28_7
(major capsid protein)

Misha28_8
(head-to-tail adaptor)

Misha28_9
(head-to-tail stopper)

Misha28_10

Misha28_11
(tail terminator)

Misha28_12
(major tail protein)

Misha28_13
(tail assembly chaperone)

Misha28_14
(tail assembly chaperone)

Misha28_15
(tape measure protein)

Misha28_16
(minor tail protein)

Misha28_17
(minor tail protein)

Misha28_18
(minor tail protein)

Misha28_19
(minor tail protein)

Misha28_20
(minor tail protein)

Misha28_21

Misha28_22

Misha28_23

Misha28_24

Misha28_25

Misha28_26

Misha28_27

Misha28_28

Misha28_29

Misha28_30

Misha28_31
(lysin A)

Misha28_32
(lysin B)

Misha28_33
(holin)

Misha28_34

Misha28_35

Misha28_36
(DNA polymerase III subunit)

Misha28_37

Misha28_38

Misha28_39

Misha28_40
(integrase)

Misha28_41

Misha28_42
(immunity repressor)

Misha28_43

Misha28_44

Misha28_45

Misha28_46

Misha28_47

Misha28_48

Misha28_49

Misha28_50

Misha28_51

Misha28_52

Misha28_53

Misha28_54

Misha28_55

Misha28_56

Misha28_57
(WhiB family transcription factor)

Misha28_58

Misha28_59

Misha28_60

Misha28_61

Misha28_62

Misha28_63

Misha28_64

Misha28_65

Misha28_66

Misha28_67

Misha28_68

Misha28_69

Misha28_70

Misha28_71
(DNA methylase)

Misha28_72

Misha28_73

Misha28_74

Misha28_75
(DNA methylase)

Misha28_76

Misha28_77

Misha28_78

Misha28_79

Misha28_80

Misha28_81

Misha28_82

Misha28_83

Misha28_84

Misha28_85

Misha28_86

Misha28_87

Misha28_88

Misha28_89

Misha28_90

Misha28_91
(glycosyltransferase)

Misha28_92

Misha28_93
(glycosyltransferase)

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMH020242
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 3.1X10^10
Date of SEA Lysate Titering Dec 14, 2016
Pitt Freezer Box# 43
Pitt Freezer Box Grid# D8
Available Files
EM PictureDownload
GenBank File for PhameratorDownload