Mycobacterium phage MisterCuddles
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Detailed Information for Phage MisterCuddles
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByEvan Witt
Year Found2012
Location FoundSt. Louis, MO USA
Finding InstitutionWashington University in St. Louis
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Unknown
Isolation Temperature37°C
GPS Coordinates38.644787 N, 90.311842 W Map
Discovery NotesBased on ClaI digest, genome size of approximately 56 kb
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedOct 10, 2013
Sequencing FacilityThe McDonnell Genome Institute at Washington University
Shotgun Sequencing Method454 Pyrosequencing
Approximate Shotgun Coverage286
Sanger Finishing Reactions4
Genome length (bp)57754
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGACGGCGC
GC Content61.4%
Fasta file available?Yes: Download fasta file
Characterization
ClusterF
SubclusterF1
Cluster Life CycleTemperate
Other Cluster Members

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Renaud18 (None)

Alexphander (F1)

ArcusAngelus (F1)

Ardmore (F1)

Awesomesauce (F1)

Batiatus (F1)

BigPhil (F1)

Bipolar (F1)

Blexus (F1)

BobaPhett (F1)

Bobi (F1)

BodEinwohner17 (F1)

Boomer (F1)

Brocalys (F1)

Bubbles123 (F1)

Burwell21 (F1)

BuzzLyseyear (F1)

ByChance (F1)

Byougenkin (F1)

Cabrinians (F1)

CaptainTrips (F1)

Cerasum (F1)

Che8 (F1)

Chuckly (F1)

Clifton (F1)

Coco12 (F1)

Cornucopia (F1)

Daenerys (F1)

Dante (F1)

DaWorst (F1)

DeadP (F1)

DillTech15 (F1)

DirtMcgirt (F1)

DLane (F1)

Donkeykong (F1)

Doomphist (F1)

Dorothy (F1)

DotProduct (F1)

Doug (F1)

Drago (F1)

DRBy19 (F1)

Eish (F1)

EleanorGeorge (F1)

Emma (F1)

Empress (F1)

Enby (F1)

Estave1 (F1)

Fancypants (F1)

Filuzino (F1)

Firehouse51 (F1)

Flathead (F1)

Florinda (F1)

Frankie (F1)

Fruitloop (F1)

Galactic (F1)

Gandalph (F1)

Geralt (F1)

Girafales (F1)

Girr (F1)

Gorge (F1)

GUmbie (F1)

Hades (F1)

Hamulus (F1)

Harley (F1)

Hegedechwinu (F1)

Ibhubesi (F1)

Inventum (F1)

IrishSherpFalk (F1)

James (F1)

Job42 (F1)

JoeyJr (F1)

Juice456 (F1)

Kenuha5 (F1)

Kersh (F1)

Kimberlium (F1)

KingMidas (F1)

Kingsley (F1)

Koella (F1)

Krakatau (F1)

KristaRAM (F1)

LastJedi (F1)

Latretium (F1)

LilMoolah (F1)

Lizziana (F1)

Llama (F1)

Llij (F1)

Lorde (F1)

Mahavrat (F1)

Mantra (F1)

Mattes (F1)

Melissauren88 (F1)

MilleniumForce (F1)

MinionDave (F1)

Minnie (F1)

Misha28 (F1)

MisterCuddles (F1)

Modragons (F1)

Mozy (F1)

Mutaforma13 (F1)

Nimbo (F1)

Nitzel (F1)

Nivrat (F1)

NormanBulbieJr (F1)

Ochi17 (F1)

OfUltron (F1)

Ogopogo (F1)

OldBen (F1)

OlympiaSaint (F1)

Ovechkin (F1)

OwlsT2W (F1)

Pacc40 (F1)

Phanphagia (F1)

PHappiness (F1)

Phasih (F1)

Phatniss (F1)

Piper2020 (F1)

Pippy (F1)

Plumbus (F1)

PMC (F1)

Poenanya (F1)

Polka14 (F1)

Pollywog (F1)

PopTart (F1)

Priscilla (F1)

QuickMath (F1)

Quico (F1)

Ramsey (F1)

RitaG (F1)

RockyHorror (F1)

Royals2015 (F1)

Ruby (F1)

Saal (F1)

Sandalphon (F1)

SassyB (F1)

Scottish (F1)

Seabastian (F1)

Seagreen (F1)

SG4 (F1)

Shauna1 (F1)

ShiLan (F1)

SimranZ1 (F1)

SiSi (F1)

Sparkdehlily (F1)

Spartacus (F1)

Spikelee (F1)

Spoonbill (F1)

StAnnes (F1)

Strokeseat (F1)

SuperGrey (F1)

SwagPigglett (F1)

Taj (F1)

TDanisky (F1)

TootsiePop (F1)

Tweety (F1)

UncleRicky (F1)

Velveteen (F1)

Veteran (F1)

Wachhund (F1)

Wee (F1)

Whouxphf (F1)

WillSterrel (F1)

XFactor (F1)

Zerg (F1)

Avani (F2)

Che9d (F2)

Demsculpinboyz (F2)

Jabbawokkie (F2)

Yoshi (F2)

Zapner (F2)

Squirty (F3)

TChen (F4)

ThetaBob (F4)

Cornie (F5)

Annotating InstitutionUniversity of South Florida
Annotation StatusIn GenBank
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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MisterCuddles_1
(terminase small subunit)

MisterCuddles_2
(terminase large subunit)

MisterCuddles_3
(portal protein)

MisterCuddles_4
(capsid maturation protease)

MisterCuddles_5
(scaffolding protein)

MisterCuddles_6
(major capsid protein)

MisterCuddles_7
(head-to-tail adaptor)

MisterCuddles_8
(head-to-tail stopper)

MisterCuddles_9

MisterCuddles_10
(tail terminator)

MisterCuddles_11
(major tail protein)

MisterCuddles_12
(tail assembly chaperone)

MisterCuddles_13
(tail assembly chaperone)

MisterCuddles_14
(tape measure protein)

MisterCuddles_15
(minor tail protein)

MisterCuddles_16
(minor tail protein)

MisterCuddles_17
(minor tail protein)

MisterCuddles_18
(minor tail protein)

MisterCuddles_19
(D-ala-D-ala carboxypeptidase)

MisterCuddles_20

MisterCuddles_21

MisterCuddles_22

MisterCuddles_23

MisterCuddles_24

MisterCuddles_25
(helix-turn-helix DNA binding domain protein)

MisterCuddles_26

MisterCuddles_27

MisterCuddles_28

MisterCuddles_29

MisterCuddles_30

MisterCuddles_31
(lysin A)

MisterCuddles_32
(HNH endonuclease)

MisterCuddles_33
(lysin B)

MisterCuddles_34
(holin)

MisterCuddles_35

MisterCuddles_36

MisterCuddles_37
(DNA polymerase)

MisterCuddles_38

MisterCuddles_39

MisterCuddles_40

MisterCuddles_41

MisterCuddles_42

MisterCuddles_43
(integrase)

MisterCuddles_44

MisterCuddles_45
(immunity repressor)

MisterCuddles_46
(helix-turn-helix DNA binding protein)

MisterCuddles_47
(helix-turn-helix DNA binding domain protein)

MisterCuddles_48

MisterCuddles_49

MisterCuddles_50
(excise)

MisterCuddles_51

MisterCuddles_52

MisterCuddles_53

MisterCuddles_54

MisterCuddles_55

MisterCuddles_56

MisterCuddles_57

MisterCuddles_58
(helix-turn-helix DNA binding domain protein)

MisterCuddles_59

MisterCuddles_60

MisterCuddles_61

MisterCuddles_62

MisterCuddles_63
(HNH endonuclease)

MisterCuddles_64
(DNA helicase)

MisterCuddles_65

MisterCuddles_66

MisterCuddles_67

MisterCuddles_68
(PAPS reductase-like domain protein)

MisterCuddles_69

MisterCuddles_70

MisterCuddles_71

MisterCuddles_72

MisterCuddles_73

MisterCuddles_74

MisterCuddles_75

MisterCuddles_76

MisterCuddles_77

MisterCuddles_78

MisterCuddles_79

MisterCuddles_80

MisterCuddles_81

MisterCuddles_82

MisterCuddles_83

MisterCuddles_84

MisterCuddles_85

MisterCuddles_86

MisterCuddles_87

MisterCuddles_88

MisterCuddles_89

MisterCuddles_90

MisterCuddles_91

MisterCuddles_92

MisterCuddles_93

MisterCuddles_94
(ribbon-helix-helix DNA binding domain protein)

MisterCuddles_96

MisterCuddles_97

MisterCuddles_98

MisterCuddles_99

MisterCuddles_100

MisterCuddles_101
(glycosyltransferase)

MisterCuddles_102

MisterCuddles_103
(glycosyltransferase)

MisterCuddles_104
(HNH endonuclease)

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK359315
Refseq NumberNone yet
Published in PaperPope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status Archived
Pitt Freezer Box# 42
Pitt Freezer Box Grid# 22
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
Final DNAMaster FileDownload
GenBank File for PhameratorDownload