Detailed Information for Phage Veteran |
Discovery Information |
Isolation Host | Mycobacterium smegmatis mc²155 |
Found By | Laura Shellooe |
Year Found | 2012 |
Location Found | Spokane, WA USA |
Finding Institution | Gonzaga University |
Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
From enriched soil sample? | Yes |
Isolation Temperature | 37°C |
GPS Coordinates | 47.66805 N, 117.400492 W Map |
Discovery Notes | Date: 9-14-12 Time: 17:30 Location: Gonzaga University campus,flower bed, flat and not close to foot traffic Depth: 3cm Soil Moisture: moist soil Temp: 28°C or 82°F |
Naming Notes | The soil that the phage was isolated from was located in a garden planted to take the shape and design of the American flag. It was this and the fact that this phage survived the many dilutions and processes of isolation, that I named this phage Veteran. |
Sequencing Information |
Sequencing Complete? | Yes |
Date Sequencing Completed | Aug 16, 2019 |
Sequencing Facility | NC State Genomic Sciences Laboratory |
Shotgun Sequencing Method | Illumina Sequencing |
Approximate Shotgun Coverage | 461 |
Genome length (bp) | 56147 |
Character of genome ends | 3' Sticky Overhang |
Overhang Length | 10 bases |
Overhang Sequence | CGGAAGGCGC |
GC Content | 61.5% |
Fasta file available? | Yes: Download fasta file |
Characterization |
Cluster | F |
Subcluster | F1 |
Cluster Life Cycle | Temperate |
Other Cluster Members |
|
Annotating Institution | Gonzaga University |
Annotation Status | In GenBank |
Plaque Notes | Plaque morphology was observed as showing plaques up to 3mm in diameter, depending on the concentration of plaques on the agar plates. The plaques had defined parameters with a cleared center and a build-up of bacteria around the edges of the plaques. |
Morphotype | Siphoviridae |
Has been Phamerated? | Yes |
Gene List |
|
Submitted Minimal DNA Master File | Download |
Publication Info |
Uploaded to GenBank? | Yes |
GenBank Accession | MT114164 |
Refseq Number | None yet |
Archiving Info |
Archiving status |
Archived |
SEA Designator |
2012GONZveteranLTS |
Pitt Freezer Box# |
36 |
Pitt Freezer Box Grid# |
14 |
Available Files |
Plaque Picture | Download |
Restriction Digest Picture | Download |
EM Picture | Download |
GenBank File for Phamerator | Download |